Advanced search
1 file | 2.76 MB Add to list

Equine hydrallantois is associated with impaired angiogenesis in the placenta

(2020) PLACENTA. 93. p.101-112
Author
Organization
Abstract
Introduction: Hydrallantois is the excessive accumulation of fluid in the allantoic cavities during the last trimester of pregnancy, leading to abdominal wall hernias, cardiovascular shock, abortion, and dystocia. It has been postulated that hydrallantois is associated with structural and/or functional changes in the chorioallantoic membrane. In the present study, we hypothesized that angiogenesis is impaired in the hydrallantoic placenta. Method: Capillary density in the hydrallantoic placenta was evaluated in the chorioallantois via immunohistochemistry for Von Willebrand Factor. Moreover, the expression of angiogenic genes was compared between equine hydrallantois and age-matched, normal placentas. Results: In the hydrallantoic samples, edema was the main pathological finding. The capillary density was significantly lower in the hydrallantoic samples than in normal placentas. The reduction in the number of vessels was associated with abnormal expression of a subset of angiogenic and hypoxia-associated genes including VEGF, VEGFR1, VEGFR2, ANGPT1, eNOS and HIF1A. We believe that the capillary density and the abnormal expression of angiogenic genes leads to tissue hypoxia (high expression of HIF1A) and edema. Finally, we identified a lower expression of genes associated with steroidogenic enzyme (CYP19A1) and estrogen receptor signaling (ESR2) in the hydrallantoic placenta. Discussion: Based on the presented data, we believe that formation of edema is due to disrupted vascular development (low number of capillaries) and hypoxia in the hydrallantoic placenta. The edema leads to further hypoxia and consequently, causes an increase in vessel permeability which leads to a gradual increase in interstitial fluid accumulation, resulting in an insufficient transplacental exchange rate and accumulation of fluid in the allantoic cavity.
Keywords
Obstetrics and Gynaecology, Developmental Biology, Reproductive Medicine, Hydrops, Hydrallantois, Fetal placenta, Equine, Angiogenesis, ENDOTHELIAL GROWTH-FACTOR, VASCULAR-PERMEABILITY FACTOR, OXIDE SYNTHASE GENE, FACTOR VEGF, TUMOR ANGIOGENESIS, FACTOR EXPRESSION, DIFFERENTIAL EXPRESSION, EMBRYONIC-DEVELOPMENT, ALLANTOIC FLUID, OVARIAN-CANCER

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.76 MB

Citation

Please use this url to cite or link to this publication:

MLA
Dini, Pouya, et al. “Equine Hydrallantois Is Associated with Impaired Angiogenesis in the Placenta.” PLACENTA, vol. 93, 2020, pp. 101–12, doi:10.1016/j.placenta.2020.03.001.
APA
Dini, P., Carossino, M., Loynachan, A. T., El-Sheikh Ali, H., Wolfsdorf, K. E., Scoggin, K. E., … Ball, B. A. (2020). Equine hydrallantois is associated with impaired angiogenesis in the placenta. PLACENTA, 93, 101–112. https://doi.org/10.1016/j.placenta.2020.03.001
Chicago author-date
Dini, Pouya, Mariano Carossino, Alan T. Loynachan, Hossam El-Sheikh Ali, Karen E. Wolfsdorf, Kirsten E. Scoggin, Peter Daels, and Barry A. Ball. 2020. “Equine Hydrallantois Is Associated with Impaired Angiogenesis in the Placenta.” PLACENTA 93: 101–12. https://doi.org/10.1016/j.placenta.2020.03.001.
Chicago author-date (all authors)
Dini, Pouya, Mariano Carossino, Alan T. Loynachan, Hossam El-Sheikh Ali, Karen E. Wolfsdorf, Kirsten E. Scoggin, Peter Daels, and Barry A. Ball. 2020. “Equine Hydrallantois Is Associated with Impaired Angiogenesis in the Placenta.” PLACENTA 93: 101–112. doi:10.1016/j.placenta.2020.03.001.
Vancouver
1.
Dini P, Carossino M, Loynachan AT, El-Sheikh Ali H, Wolfsdorf KE, Scoggin KE, et al. Equine hydrallantois is associated with impaired angiogenesis in the placenta. PLACENTA. 2020;93:101–12.
IEEE
[1]
P. Dini et al., “Equine hydrallantois is associated with impaired angiogenesis in the placenta,” PLACENTA, vol. 93, pp. 101–112, 2020.
@article{8659020,
  abstract     = {{Introduction: Hydrallantois is the excessive accumulation of fluid in the allantoic cavities during the last trimester of pregnancy, leading to abdominal wall hernias, cardiovascular shock, abortion, and dystocia. It has been postulated that hydrallantois is associated with structural and/or functional changes in the chorioallantoic membrane. In the present study, we hypothesized that angiogenesis is impaired in the hydrallantoic placenta.

Method: Capillary density in the hydrallantoic placenta was evaluated in the chorioallantois via immunohistochemistry for Von Willebrand Factor. Moreover, the expression of angiogenic genes was compared between equine hydrallantois and age-matched, normal placentas.

Results: In the hydrallantoic samples, edema was the main pathological finding. The capillary density was significantly lower in the hydrallantoic samples than in normal placentas. The reduction in the number of vessels was associated with abnormal expression of a subset of angiogenic and hypoxia-associated genes including VEGF, VEGFR1, VEGFR2, ANGPT1, eNOS and HIF1A. We believe that the capillary density and the abnormal expression of angiogenic genes leads to tissue hypoxia (high expression of HIF1A) and edema. Finally, we identified a lower expression of genes associated with steroidogenic enzyme (CYP19A1) and estrogen receptor signaling (ESR2) in the hydrallantoic placenta.

Discussion: Based on the presented data, we believe that formation of edema is due to disrupted vascular development (low number of capillaries) and hypoxia in the hydrallantoic placenta. The edema leads to further hypoxia and consequently, causes an increase in vessel permeability which leads to a gradual increase in interstitial fluid accumulation, resulting in an insufficient transplacental exchange rate and accumulation of fluid in the allantoic cavity.}},
  author       = {{Dini, Pouya and Carossino, Mariano and Loynachan, Alan T. and El-Sheikh Ali, Hossam and Wolfsdorf, Karen E. and Scoggin, Kirsten E. and Daels, Peter and Ball, Barry A.}},
  issn         = {{0143-4004}},
  journal      = {{PLACENTA}},
  keywords     = {{Obstetrics and Gynaecology,Developmental Biology,Reproductive Medicine,Hydrops,Hydrallantois,Fetal placenta,Equine,Angiogenesis,ENDOTHELIAL GROWTH-FACTOR,VASCULAR-PERMEABILITY FACTOR,OXIDE SYNTHASE GENE,FACTOR VEGF,TUMOR ANGIOGENESIS,FACTOR EXPRESSION,DIFFERENTIAL EXPRESSION,EMBRYONIC-DEVELOPMENT,ALLANTOIC FLUID,OVARIAN-CANCER}},
  language     = {{eng}},
  pages        = {{101--112}},
  title        = {{Equine hydrallantois is associated with impaired angiogenesis in the placenta}},
  url          = {{http://dx.doi.org/10.1016/j.placenta.2020.03.001}},
  volume       = {{93}},
  year         = {{2020}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: