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Transcriptomic analysis reveals the key regulators and molecular mechanisms underlying myometrial activation during equine placentitis

(2020) BIOLOGY OF REPRODUCTION. 102(6). p.1306-1325
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Abstract
The key event in placentitis-induced preterm labor is myometrial activation with the subsequent initiation of labor. However, the molecular mechanisms underlying myometrial activation are not fully understood in the mares. Therefore, the equine myometrial transcriptome was characterized during placentitis (290.0 ± 1.52 days of GA, n = 5) and the prepartum period (330 days of GA, n = 3) in comparison to normal pregnant mares (289.8 ± 2.18 days of GA, n = 4). Transcriptome analysis identified 596 and 290 DEGs in the myometrium during placentitis and the prepartum period, respectively, with 138 DEGs in common. The placentitis DEGs included eight genes (MMP1, MMP8, S100A9, S100A8, PI3, APOBEC3Z1B, RETN, and CXCL2) that are exclusively expressed in the inflamed myometrium. Pathway analysis elucidated that inflammatory signaling, Toll-like receptor signaling, and apoptosis pathways dominate myometrial activation during placentitis. The prepartum myometrium was associated with overexpression of inflammatory signaling, oxidative stress, and 5-hydroxytryptamine degradation. Gene ontology enrichment analysis identified several chemoattractant factors in the myometrium during placentitis and prepartum period, including CCL2, CXCL1, CXCL3, and CXCL6 in common. Upstream regulator analysis revealed 19 potential upstream regulators in placentitis dataset including transcription regulators (E2F1, FOXM1, HIF1A, JUNB, NFKB1A, and STAT1), transmembrane receptors (FAS, ICAM1, SELP, TLR2, and TYROBP), growth factors (HGF and TGFB3), enzymes (PTGS2 and PRKCP), and others (S100A8, S100A9, CD44, and C5AR1). Additionally, three upstream regulators (STAT3, EGR1, and F2R) were identified in the prepartum dataset. These findings revealed the key regulators and pathways underlying myometrial activation during placentitis, which aid in understanding the disease and facilitate the development of efficacious therapies.
Keywords
quine placentitis, myometrium, pregnancy, prepartum period, preterm labor, transcriptome, SPONTANEOUS HUMAN LABOR, TOLL-LIKE RECEPTORS, GENE-EXPRESSION, PRETERM LABOR, PROGESTERONE-RECEPTORS, ASCENDING PLACENTITIS, UTERINE CONTRACTILITY, OXIDATIVE STRESS, HIGH-THROUGHPUT, RAT MYOMETRIUM

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MLA
El-Sheikh Ali, H., et al. “Transcriptomic Analysis Reveals the Key Regulators and Molecular Mechanisms Underlying Myometrial Activation during Equine Placentitis.” BIOLOGY OF REPRODUCTION, vol. 102, no. 6, 2020, pp. 1306–25, doi:10.1093/biolre/ioaa020.
APA
El-Sheikh Ali, H., Boakari, Y. L., Loux, S. C., Dini, P., Scoggin, K. E., Esteller-Vico, A., … Ball, B. A. (2020). Transcriptomic analysis reveals the key regulators and molecular mechanisms underlying myometrial activation during equine placentitis. BIOLOGY OF REPRODUCTION, 102(6), 1306–1325. https://doi.org/10.1093/biolre/ioaa020
Chicago author-date
El-Sheikh Ali, H., Y. L. Boakari, S. C. Loux, Pouya Dini, K. E. Scoggin, A. Esteller-Vico, T. Kalbfleisch, and B. A. Ball. 2020. “Transcriptomic Analysis Reveals the Key Regulators and Molecular Mechanisms Underlying Myometrial Activation during Equine Placentitis.” BIOLOGY OF REPRODUCTION 102 (6): 1306–25. https://doi.org/10.1093/biolre/ioaa020.
Chicago author-date (all authors)
El-Sheikh Ali, H., Y. L. Boakari, S. C. Loux, Pouya Dini, K. E. Scoggin, A. Esteller-Vico, T. Kalbfleisch, and B. A. Ball. 2020. “Transcriptomic Analysis Reveals the Key Regulators and Molecular Mechanisms Underlying Myometrial Activation during Equine Placentitis.” BIOLOGY OF REPRODUCTION 102 (6): 1306–1325. doi:10.1093/biolre/ioaa020.
Vancouver
1.
El-Sheikh Ali H, Boakari YL, Loux SC, Dini P, Scoggin KE, Esteller-Vico A, et al. Transcriptomic analysis reveals the key regulators and molecular mechanisms underlying myometrial activation during equine placentitis. BIOLOGY OF REPRODUCTION. 2020;102(6):1306–25.
IEEE
[1]
H. El-Sheikh Ali et al., “Transcriptomic analysis reveals the key regulators and molecular mechanisms underlying myometrial activation during equine placentitis,” BIOLOGY OF REPRODUCTION, vol. 102, no. 6, pp. 1306–1325, 2020.
@article{8659018,
  abstract     = {{The key event in placentitis-induced preterm labor is myometrial activation with the subsequent initiation of labor. However, the molecular mechanisms underlying myometrial activation are not fully understood in the mares. Therefore, the equine myometrial transcriptome was characterized during placentitis (290.0 ± 1.52 days of GA, n = 5) and the prepartum period (330 days of GA, n = 3) in comparison to normal pregnant mares (289.8 ± 2.18 days of GA, n = 4). Transcriptome analysis identified 596 and 290 DEGs in the myometrium during placentitis and the prepartum period, respectively, with 138 DEGs in common. The placentitis DEGs included eight genes (MMP1, MMP8, S100A9, S100A8, PI3, APOBEC3Z1B, RETN, and CXCL2) that are exclusively expressed in the inflamed myometrium. Pathway analysis elucidated that inflammatory signaling, Toll-like receptor signaling, and apoptosis pathways dominate myometrial activation during placentitis. The prepartum myometrium was associated with overexpression of inflammatory signaling, oxidative stress, and 5-hydroxytryptamine degradation. Gene ontology enrichment analysis identified several chemoattractant factors in the myometrium during placentitis and prepartum period, including CCL2, CXCL1, CXCL3, and CXCL6 in common. Upstream regulator analysis revealed 19 potential upstream regulators in placentitis dataset including transcription regulators (E2F1, FOXM1, HIF1A, JUNB, NFKB1A, and STAT1), transmembrane receptors (FAS, ICAM1, SELP, TLR2, and TYROBP), growth factors (HGF and TGFB3), enzymes (PTGS2 and PRKCP), and others (S100A8, S100A9, CD44, and C5AR1). Additionally, three upstream regulators (STAT3, EGR1, and F2R) were identified in the prepartum dataset. These findings revealed the key regulators and pathways underlying myometrial activation during placentitis, which aid in understanding the disease and facilitate the development of efficacious therapies.}},
  author       = {{El-Sheikh Ali, H. and Boakari, Y. L. and Loux, S. C. and Dini, Pouya and Scoggin, K. E. and Esteller-Vico, A. and Kalbfleisch, T. and Ball, B. A.}},
  issn         = {{0006-3363}},
  journal      = {{BIOLOGY OF REPRODUCTION}},
  keywords     = {{quine placentitis,myometrium,pregnancy,prepartum period,preterm labor,transcriptome,SPONTANEOUS HUMAN LABOR,TOLL-LIKE RECEPTORS,GENE-EXPRESSION,PRETERM LABOR,PROGESTERONE-RECEPTORS,ASCENDING PLACENTITIS,UTERINE CONTRACTILITY,OXIDATIVE STRESS,HIGH-THROUGHPUT,RAT MYOMETRIUM}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{1306--1325}},
  title        = {{Transcriptomic analysis reveals the key regulators and molecular mechanisms underlying myometrial activation during equine placentitis}},
  url          = {{http://doi.org/10.1093/biolre/ioaa020}},
  volume       = {{102}},
  year         = {{2020}},
}

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