Target of rapamycin (TOR) determines appendage size during pupa formation of the red flour beetle Tribolium castaneum
- Author
- Xianyu Lin, Kristof De Schutter (UGent) , Silvia Chafino, Xavier Franch-Marro, David Martin and Guy Smagghe (UGent)
- Organization
- Abstract
- The adult body size is species-specific and controlled by complex interactions between hormones and the IIS/TOR pathway. To analyze the role of target of rapamycin (TOR) in the growth and development of the insect, expression levels of TOR were silenced in the model and pest insect red flour beetle, Tribolium castaneum. Injection of dsRNA into the last larval instar decreased pupal mass and size, while the amount of food intake by the larvae was not affected. These results place TcTOR downstream of nutrition as a transducer for nutritional signals to increase larval growth. In addition, TcTOR-silencing notably decreased the size of the adult appendages. Analysis of the wings and elytra revealed a decrease in cell size and number of these appendages in the TcTOR-silenced insects. This reduction in size was correlated with a decrease of transcriptional levels of marker genes controlling the cell cycle. Altogether, these results suggest a pivotal role for TcTOR in integrating nutritional signals and regulation of body and appendages growth.
- Keywords
- Target of rapamycin, Body size, Adult structures development, Cell size and cell number, RNA interference, Tribolium castaneum, INSULIN-LIKE PEPTIDES, PHOSPHOINOSITIDE 3-KINASE, NUTRITIONAL SIGNALS, DROSOPHILA HOMOLOG, GROWTH, CELL, METAMORPHOSIS, TRANSDUCTION, SECRETION, RECEPTOR
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8658072
- MLA
- Lin, Xianyu, et al. “Target of Rapamycin (TOR) Determines Appendage Size during Pupa Formation of the Red Flour Beetle Tribolium Castaneum.” JOURNAL OF INSECT PHYSIOLOGY, vol. 117, 2019, doi:10.1016/j.jinsphys.2019.103902.
- APA
- Lin, X., De Schutter, K., Chafino, S., Franch-Marro, X., Martin, D., & Smagghe, G. (2019). Target of rapamycin (TOR) determines appendage size during pupa formation of the red flour beetle Tribolium castaneum. JOURNAL OF INSECT PHYSIOLOGY, 117. https://doi.org/10.1016/j.jinsphys.2019.103902
- Chicago author-date
- Lin, Xianyu, Kristof De Schutter, Silvia Chafino, Xavier Franch-Marro, David Martin, and Guy Smagghe. 2019. “Target of Rapamycin (TOR) Determines Appendage Size during Pupa Formation of the Red Flour Beetle Tribolium Castaneum.” JOURNAL OF INSECT PHYSIOLOGY 117. https://doi.org/10.1016/j.jinsphys.2019.103902.
- Chicago author-date (all authors)
- Lin, Xianyu, Kristof De Schutter, Silvia Chafino, Xavier Franch-Marro, David Martin, and Guy Smagghe. 2019. “Target of Rapamycin (TOR) Determines Appendage Size during Pupa Formation of the Red Flour Beetle Tribolium Castaneum.” JOURNAL OF INSECT PHYSIOLOGY 117. doi:10.1016/j.jinsphys.2019.103902.
- Vancouver
- 1.Lin X, De Schutter K, Chafino S, Franch-Marro X, Martin D, Smagghe G. Target of rapamycin (TOR) determines appendage size during pupa formation of the red flour beetle Tribolium castaneum. JOURNAL OF INSECT PHYSIOLOGY. 2019;117.
- IEEE
- [1]X. Lin, K. De Schutter, S. Chafino, X. Franch-Marro, D. Martin, and G. Smagghe, “Target of rapamycin (TOR) determines appendage size during pupa formation of the red flour beetle Tribolium castaneum,” JOURNAL OF INSECT PHYSIOLOGY, vol. 117, 2019.
@article{8658072,
abstract = {{The adult body size is species-specific and controlled by complex interactions between hormones and the IIS/TOR pathway. To analyze the role of target of rapamycin (TOR) in the growth and development of the insect, expression levels of TOR were silenced in the model and pest insect red flour beetle, Tribolium castaneum. Injection of dsRNA into the last larval instar decreased pupal mass and size, while the amount of food intake by the larvae was not affected. These results place TcTOR downstream of nutrition as a transducer for nutritional signals to increase larval growth. In addition, TcTOR-silencing notably decreased the size of the adult appendages. Analysis of the wings and elytra revealed a decrease in cell size and number of these appendages in the TcTOR-silenced insects. This reduction in size was correlated with a decrease of transcriptional levels of marker genes controlling the cell cycle. Altogether, these results suggest a pivotal role for TcTOR in integrating nutritional signals and regulation of body and appendages growth.}},
articleno = {{103902}},
author = {{Lin, Xianyu and De Schutter, Kristof and Chafino, Silvia and Franch-Marro, Xavier and Martin, David and Smagghe, Guy}},
issn = {{0022-1910}},
journal = {{JOURNAL OF INSECT PHYSIOLOGY}},
keywords = {{Target of rapamycin,Body size,Adult structures development,Cell size and cell number,RNA interference,Tribolium castaneum,INSULIN-LIKE PEPTIDES,PHOSPHOINOSITIDE 3-KINASE,NUTRITIONAL SIGNALS,DROSOPHILA HOMOLOG,GROWTH,CELL,METAMORPHOSIS,TRANSDUCTION,SECRETION,RECEPTOR}},
language = {{eng}},
pages = {{8}},
title = {{Target of rapamycin (TOR) determines appendage size during pupa formation of the red flour beetle Tribolium castaneum}},
url = {{http://doi.org/10.1016/j.jinsphys.2019.103902}},
volume = {{117}},
year = {{2019}},
}
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