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LC-MS/MS methodology for simultaneous determination of patulin and citrinin in urine and plasma applied to a pilot study in colorectal cancer patients

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Abstract
Biomarker-driven research has been proposed as a successful method to assess the exposure of individuals to xenobiotics, including mycotoxins, through estimation of their metabolites in biological fluids. A methodology to determine patulin (PAT) and citrinin (CIT) in human urine and plasma using liquid chromatography coupled to tandem mass spectrometry was developed and validated in the present study. Selectivity/specificity, linearity, limit of detection and quantification, apparent recovery, intraday- and interday-precision and measurement uncertainty were investigated for validation purposes. Finally, the method was used to analyze human urine (n = 100) and plasma (n = 100) case-control samples, where 50 samples originated from colorectal cancer patients and 50 from age/sex-matched controls. This case-control study revealed that PAT was not detected in urine samples, however occurred in 25% of the analysed plasma samples with an average concentration of 11.62 +/- 6.67 ng/mL in the positive samples. CIT was found in urine samples (74%) and plasma samples (36%) with average concentrations in the positive samples of 0.45 +/- 0.24 ng/mL and 0.49 +/- 0.2 ng/mL respectively. No statistically significant difference of PAT and CIT concentration among colorectal cancer and control patients (p > 0.05) was observed.
Keywords
LC-MS/MS, Mycotoxin exposure, Colorectal cancer, Plasma, Urine, MYCOTOXIN BIOMARKERS, MULTIPLE MYCOTOXINS, QUECHERS EXTRACTION, OCHRATOXIN-A, MASKED FORMS, DNA-DAMAGE, EXPOSURE, MS, DEOXYNIVALENOL, STABILITY

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MLA
Ouhibi, Salma, et al. “LC-MS/MS Methodology for Simultaneous Determination of Patulin and Citrinin in Urine and Plasma Applied to a Pilot Study in Colorectal Cancer Patients.” FOOD AND CHEMICAL TOXICOLOGY, vol. 136, 2020.
APA
Ouhibi, S., Vidal Corominas, A., Gali, R., Martins, C., Hedhili, A., De Saeger, S., & De Boevre, M. (2020). LC-MS/MS methodology for simultaneous determination of patulin and citrinin in urine and plasma applied to a pilot study in colorectal cancer patients. FOOD AND CHEMICAL TOXICOLOGY, 136.
Chicago author-date
Ouhibi, Salma, Arnau Vidal Corominas, Ridha Gali, Carla Martins, Abderrazzek Hedhili, Sarah De Saeger, and Marthe De Boevre. 2020. “LC-MS/MS Methodology for Simultaneous Determination of Patulin and Citrinin in Urine and Plasma Applied to a Pilot Study in Colorectal Cancer Patients.” FOOD AND CHEMICAL TOXICOLOGY 136.
Chicago author-date (all authors)
Ouhibi, Salma, Arnau Vidal Corominas, Ridha Gali, Carla Martins, Abderrazzek Hedhili, Sarah De Saeger, and Marthe De Boevre. 2020. “LC-MS/MS Methodology for Simultaneous Determination of Patulin and Citrinin in Urine and Plasma Applied to a Pilot Study in Colorectal Cancer Patients.” FOOD AND CHEMICAL TOXICOLOGY 136.
Vancouver
1.
Ouhibi S, Vidal Corominas A, Gali R, Martins C, Hedhili A, De Saeger S, et al. LC-MS/MS methodology for simultaneous determination of patulin and citrinin in urine and plasma applied to a pilot study in colorectal cancer patients. FOOD AND CHEMICAL TOXICOLOGY. 2020;136.
IEEE
[1]
S. Ouhibi et al., “LC-MS/MS methodology for simultaneous determination of patulin and citrinin in urine and plasma applied to a pilot study in colorectal cancer patients,” FOOD AND CHEMICAL TOXICOLOGY, vol. 136, 2020.
@article{8656790,
  abstract     = {Biomarker-driven research has been proposed as a successful method to assess the exposure of individuals to xenobiotics, including mycotoxins, through estimation of their metabolites in biological fluids. A methodology to determine patulin (PAT) and citrinin (CIT) in human urine and plasma using liquid chromatography coupled to tandem mass spectrometry was developed and validated in the present study. Selectivity/specificity, linearity, limit of detection and quantification, apparent recovery, intraday- and interday-precision and measurement uncertainty were investigated for validation purposes. Finally, the method was used to analyze human urine (n = 100) and plasma (n = 100) case-control samples, where 50 samples originated from colorectal cancer patients and 50 from age/sex-matched controls. This case-control study revealed that PAT was not detected in urine samples, however occurred in 25% of the analysed plasma samples with an average concentration of 11.62 +/- 6.67 ng/mL in the positive samples. CIT was found in urine samples (74%) and plasma samples (36%) with average concentrations in the positive samples of 0.45 +/- 0.24 ng/mL and 0.49 +/- 0.2 ng/mL respectively. No statistically significant difference of PAT and CIT concentration among colorectal cancer and control patients (p > 0.05) was observed.},
  articleno    = {110994},
  author       = {Ouhibi, Salma and Vidal Corominas, Arnau and Gali, Ridha and Martins, Carla and Hedhili, Abderrazzek and De Saeger, Sarah and De Boevre, Marthe},
  issn         = {0278-6915},
  journal      = {FOOD AND CHEMICAL TOXICOLOGY},
  keywords     = {LC-MS/MS,Mycotoxin exposure,Colorectal cancer,Plasma,Urine,MYCOTOXIN BIOMARKERS,MULTIPLE MYCOTOXINS,QUECHERS EXTRACTION,OCHRATOXIN-A,MASKED FORMS,DNA-DAMAGE,EXPOSURE,MS,DEOXYNIVALENOL,STABILITY},
  language     = {eng},
  pages        = {8},
  title        = {LC-MS/MS methodology for simultaneous determination of patulin and citrinin in urine and plasma applied to a pilot study in colorectal cancer patients},
  url          = {http://dx.doi.org/10.1016/j.fct.2019.110994},
  volume       = {136},
  year         = {2020},
}

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