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Resistance risk assessment of the novel complex II inhibitor pyflubumide in the polyphagous pest Tetranychus urticae

(2020) JOURNAL OF PEST SCIENCE. 93(3). p.1085-1096
Author
Organization
Project
  • POLYADAPT (Molecular-genetic mechanisms of extreme adaptation in a polyphagous agricultural pest)
  • SuperPests (Innovative tools for rational control of the most difficult-to-manage pests (super pests) and the diseases they transmit)
Abstract
Pyflubumide is a novel selective carboxanilide acaricide that inhibits mitochondrial complex II of spider mite species such as Tetranychus urticae. We explored the baseline toxicity and potential cross-resistance risk of pyflubumide in a reference panel of T. urticae strains resistant to various acaricides with different modes of action. A cyenopyrafen-resistant strain (JPR) was identified as the only strain with low-to-moderate level of cross-resistance to pyflubumide (LC50 = 49.07 mg/L). In a resistance risk assessment approach, JPR was subsequently selected which led to two highly resistant strains JPR-R1 (RR = 466.7) and JPR-R2 (RR = 614.8). Interestingly, compared to adult females, resistance was much less pronounced in adult males and eggs of the two JPR-R strains. In order to elucidate resistance mechanisms, we first sequenced the complex II subunits in susceptible and resistant strains, but target-site insensitivity could not be detected. In contrast, synergism/antagonism experiments strongly suggested that cytochrome P450 monooxygenases are involved in pyflubumide resistance. We therefore conducted genome-wide gene expression experiments to investigate constitutive and induced expression patterns and documented the overexpression of five cytochrome P450 and four carboxyl/choline esterase genes in the JPR-R strains after pyflubumide exposure. Together, we provide a first resistance risk assessment of a novel complex II inhibitor and provide first evidence for metabolic resistance mediated by cytochrome P450s in T. urticae.
Keywords
Pyflubumide, Pesticide selection, Complex II, Cytochrome P450, Cross-resistance, Cyenopyrafen, CROSS-RESISTANCE, KOCH ACARI, DOSAGE-COMPENSATION, MOLECULAR ANALYSIS, EVOLUTION, MODE, CYENOPYRAFEN, INHERITANCE, MECHANISMS, SUSCEPTIBILITY

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Citation

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MLA
Fotoukkiaii, Seyedeh Masoumeh, et al. “Resistance Risk Assessment of the Novel Complex II Inhibitor Pyflubumide in the Polyphagous Pest Tetranychus Urticae.” JOURNAL OF PEST SCIENCE, vol. 93, no. 3, 2020, pp. 1085–96, doi:10.1007/s10340-020-01213-x.
APA
Fotoukkiaii, S. M., Mermans, C., Wybouw, N., & Van Leeuwen, T. (2020). Resistance risk assessment of the novel complex II inhibitor pyflubumide in the polyphagous pest Tetranychus urticae. JOURNAL OF PEST SCIENCE, 93(3), 1085–1096. https://doi.org/10.1007/s10340-020-01213-x
Chicago author-date
Fotoukkiaii, Seyedeh Masoumeh, Catherine Mermans, Nicky Wybouw, and Thomas Van Leeuwen. 2020. “Resistance Risk Assessment of the Novel Complex II Inhibitor Pyflubumide in the Polyphagous Pest Tetranychus Urticae.” JOURNAL OF PEST SCIENCE 93 (3): 1085–96. https://doi.org/10.1007/s10340-020-01213-x.
Chicago author-date (all authors)
Fotoukkiaii, Seyedeh Masoumeh, Catherine Mermans, Nicky Wybouw, and Thomas Van Leeuwen. 2020. “Resistance Risk Assessment of the Novel Complex II Inhibitor Pyflubumide in the Polyphagous Pest Tetranychus Urticae.” JOURNAL OF PEST SCIENCE 93 (3): 1085–1096. doi:10.1007/s10340-020-01213-x.
Vancouver
1.
Fotoukkiaii SM, Mermans C, Wybouw N, Van Leeuwen T. Resistance risk assessment of the novel complex II inhibitor pyflubumide in the polyphagous pest Tetranychus urticae. JOURNAL OF PEST SCIENCE. 2020;93(3):1085–96.
IEEE
[1]
S. M. Fotoukkiaii, C. Mermans, N. Wybouw, and T. Van Leeuwen, “Resistance risk assessment of the novel complex II inhibitor pyflubumide in the polyphagous pest Tetranychus urticae,” JOURNAL OF PEST SCIENCE, vol. 93, no. 3, pp. 1085–1096, 2020.
@article{8656650,
  abstract     = {{Pyflubumide is a novel selective carboxanilide acaricide that inhibits mitochondrial complex II of spider mite species such as Tetranychus urticae. We explored the baseline toxicity and potential cross-resistance risk of pyflubumide in a reference panel of T. urticae strains resistant to various acaricides with different modes of action. A cyenopyrafen-resistant strain (JPR) was identified as the only strain with low-to-moderate level of cross-resistance to pyflubumide (LC50 = 49.07 mg/L). In a resistance risk assessment approach, JPR was subsequently selected which led to two highly resistant strains JPR-R1 (RR = 466.7) and JPR-R2 (RR = 614.8). Interestingly, compared to adult females, resistance was much less pronounced in adult males and eggs of the two JPR-R strains. In order to elucidate resistance mechanisms, we first sequenced the complex II subunits in susceptible and resistant strains, but target-site insensitivity could not be detected. In contrast, synergism/antagonism experiments strongly suggested that cytochrome P450 monooxygenases are involved in pyflubumide resistance. We therefore conducted genome-wide gene expression experiments to investigate constitutive and induced expression patterns and documented the overexpression of five cytochrome P450 and four carboxyl/choline esterase genes in the JPR-R strains after pyflubumide exposure. Together, we provide a first resistance risk assessment of a novel complex II inhibitor and provide first evidence for metabolic resistance mediated by cytochrome P450s in T. urticae.}},
  author       = {{Fotoukkiaii, Seyedeh Masoumeh and Mermans, Catherine and Wybouw, Nicky and Van Leeuwen, Thomas}},
  issn         = {{1612-4758}},
  journal      = {{JOURNAL OF PEST SCIENCE}},
  keywords     = {{Pyflubumide,Pesticide selection,Complex II,Cytochrome P450,Cross-resistance,Cyenopyrafen,CROSS-RESISTANCE,KOCH ACARI,DOSAGE-COMPENSATION,MOLECULAR ANALYSIS,EVOLUTION,MODE,CYENOPYRAFEN,INHERITANCE,MECHANISMS,SUSCEPTIBILITY}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{1085--1096}},
  title        = {{Resistance risk assessment of the novel complex II inhibitor pyflubumide in the polyphagous pest Tetranychus urticae}},
  url          = {{http://dx.doi.org/10.1007/s10340-020-01213-x}},
  volume       = {{93}},
  year         = {{2020}},
}

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