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Cryopreservation of equine oocytes: looking into the crystal ball

Tine De Coster (UGent) , Daniel Angel Velez (UGent) , Ann Van Soom (UGent) , Henri Woelders and Katrien Smits (UGent)
(2020) REPRODUCTION FERTILITY AND DEVELOPMENT. 32(5). p.453-467
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Abstract
In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application. Oocyte vitrification is affected by several technical and biological factors. In the horse, short exposure of immature oocytes to the combination of permeating and non-permeating cryoprotective agents has been associated with the best results so far. High cooling and warming rates are also crucial and can be obtained by using minimal volumes and open cryodevices. Vitrification of in vivo-matured oocytes has yielded better results, but is less practical. The presence of the corona radiata seems to partially protect those factors that are necessary for the construction of the normal spindle and for chromosome alignment, but multiple layers of cumulus cells may impair permeation of cryoprotective agents. In addition to the spindle, the oolemma and mitochondria are also particularly sensitive to vitrification damage, which should be minimised in future vitrification procedures. This review presents promising protocols and novel strategies in equine oocyte vitrification, with a focus on blastocyst development and foal production as most reliable outcome parameters.
Keywords
Biotechnology, Developmental Biology, Animal Science and Zoology, Genetics, Molecular Biology, Endocrinology, Reproductive Medicine, blastocyst, cumulus cells, intracytoplasmic sperm injection, maturation, vitrification, IN-VITRO MATURATION, CYTOPLASMIC LIPID DROPLETS, GERMINAL VESICLE STAGE, BOVINE OOCYTES, MOUSE OOCYTES, HORSE OOCYTES, EMBRYO DEVELOPMENT, CUMULUS CELLS, DEVELOPMENTAL COMPETENCE, CRYOPROTECTIVE AGENTS

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MLA
De Coster, Tine, et al. “Cryopreservation of Equine Oocytes: Looking into the Crystal Ball.” REPRODUCTION FERTILITY AND DEVELOPMENT, vol. 32, no. 5, 2020, pp. 453–67, doi:10.1071/rd19229.
APA
De Coster, T., Angel Velez, D., Van Soom, A., Woelders, H., & Smits, K. (2020). Cryopreservation of equine oocytes: looking into the crystal ball. REPRODUCTION FERTILITY AND DEVELOPMENT, 32(5), 453–467. https://doi.org/10.1071/rd19229
Chicago author-date
De Coster, Tine, Daniel Angel Velez, Ann Van Soom, Henri Woelders, and Katrien Smits. 2020. “Cryopreservation of Equine Oocytes: Looking into the Crystal Ball.” REPRODUCTION FERTILITY AND DEVELOPMENT 32 (5): 453–67. https://doi.org/10.1071/rd19229.
Chicago author-date (all authors)
De Coster, Tine, Daniel Angel Velez, Ann Van Soom, Henri Woelders, and Katrien Smits. 2020. “Cryopreservation of Equine Oocytes: Looking into the Crystal Ball.” REPRODUCTION FERTILITY AND DEVELOPMENT 32 (5): 453–467. doi:10.1071/rd19229.
Vancouver
1.
De Coster T, Angel Velez D, Van Soom A, Woelders H, Smits K. Cryopreservation of equine oocytes: looking into the crystal ball. REPRODUCTION FERTILITY AND DEVELOPMENT. 2020;32(5):453–67.
IEEE
[1]
T. De Coster, D. Angel Velez, A. Van Soom, H. Woelders, and K. Smits, “Cryopreservation of equine oocytes: looking into the crystal ball,” REPRODUCTION FERTILITY AND DEVELOPMENT, vol. 32, no. 5, pp. 453–467, 2020.
@article{8656582,
  abstract     = {{In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application. Oocyte vitrification is affected by several technical and biological factors. In the horse, short exposure of immature oocytes to the combination of permeating and non-permeating cryoprotective agents has been associated with the best results so far. High cooling and warming rates are also crucial and can be obtained by using minimal volumes and open cryodevices. Vitrification of in vivo-matured oocytes has yielded better results, but is less practical. The presence of the corona radiata seems to partially protect those factors that are necessary for the construction of the normal spindle and for chromosome alignment, but multiple layers of cumulus cells may impair permeation of cryoprotective agents. In addition to the spindle, the oolemma and mitochondria are also particularly sensitive to vitrification damage, which should be minimised in future vitrification procedures. This review presents promising protocols and novel strategies in equine oocyte vitrification, with a focus on blastocyst development and foal production as most reliable outcome parameters.}},
  author       = {{De Coster, Tine and Angel Velez, Daniel and Van Soom, Ann and Woelders, Henri and Smits, Katrien}},
  issn         = {{1031-3613}},
  journal      = {{REPRODUCTION FERTILITY AND DEVELOPMENT}},
  keywords     = {{Biotechnology,Developmental Biology,Animal Science and Zoology,Genetics,Molecular Biology,Endocrinology,Reproductive Medicine,blastocyst,cumulus cells,intracytoplasmic sperm injection,maturation,vitrification,IN-VITRO MATURATION,CYTOPLASMIC LIPID DROPLETS,GERMINAL VESICLE STAGE,BOVINE OOCYTES,MOUSE OOCYTES,HORSE OOCYTES,EMBRYO DEVELOPMENT,CUMULUS CELLS,DEVELOPMENTAL COMPETENCE,CRYOPROTECTIVE AGENTS}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{453--467}},
  title        = {{Cryopreservation of equine oocytes: looking into the crystal ball}},
  url          = {{http://doi.org/10.1071/rd19229}},
  volume       = {{32}},
  year         = {{2020}},
}
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