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Maternal recognition of pregnancy in the horse : are MicroRNAs the secret messengers?

Katrien Smits (UGent) , Yannick Gansemans (UGent) , Laurentijn Tilleman (UGent) , Filip Van Nieuwerburgh (UGent) , Margot Van de Velde (UGent) , Ilse Gerits (UGent) , Cyrillus Ververs (UGent) , Kim Roels (UGent) , Jan Govaere (UGent) , Luc Peelman (UGent) , et al.
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Abstract
The signal for maternal recognition of pregnancy (MRP) has still not been identified in the horse. High-throughput molecular biology at the embryo-maternal interface has substantially contributed to the knowledge on pathways affected during MRP, but an integrated study in which proteomics, transcriptomics and miRNA expression can be linked directly is currently lacking. The aim of this study was to provide such analysis. Endometrial biopsies, uterine fluid, embryonic tissues, and yolk sac fluid were collected 13 days after ovulation during pregnant and control cycles from the same mares. Micro-RNA-Sequencing was performed on all collected samples, mRNA-Sequencing on the same tissue samples and mass spectrometry was conducted previously on the same fluid samples. Differential expression of miRNA, mRNA and proteins showed high conformity with literature and confirmed involvement in pregnancy establishment, embryo quality, steroid synthesis and prostaglandin regulation, but the link between differential miRNAs and their targets was limited and did not indicate the identity of an unequivocal signal for MRP in the horse. Differential expression at the embryo-maternal interface was prominent, highlighting a potential role of miRNAs in embryo-maternal communication during early pregnancy in the horse. These data provide a strong basis for future targeted studies.
Keywords
Physical and Theoretical Chemistry, Inorganic Chemistry, Organic Chemistry, Spectroscopy, Molecular Biology, Catalysis, General Medicine, Computer Science Applications, maternal recognition of pregnancy, horse, RNA-Sequencing, proteomics, microRNA, EQUINE ENDOMETRIUM, ESTROUS-CYCLE, DIFFERENTIAL EXPRESSION, TROPHOBLASTIC CELLS, FOLLICULAR-FLUID, IMPLANTATION, OXYTOCIN, MIGRATION, INVASION, RECEPTOR

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MLA
Smits, Katrien, et al. “Maternal Recognition of Pregnancy in the Horse : Are MicroRNAs the Secret Messengers?” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 2, 2020, doi:10.3390/ijms21020419.
APA
Smits, K., Gansemans, Y., Tilleman, L., Van Nieuwerburgh, F., Van de Velde, M., Gerits, I., … Van Soom, A. (2020). Maternal recognition of pregnancy in the horse : are MicroRNAs the secret messengers? INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(2). https://doi.org/10.3390/ijms21020419
Chicago author-date
Smits, Katrien, Yannick Gansemans, Laurentijn Tilleman, Filip Van Nieuwerburgh, Margot Van de Velde, Ilse Gerits, Cyrillus Ververs, et al. 2020. “Maternal Recognition of Pregnancy in the Horse : Are MicroRNAs the Secret Messengers?” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 (2). https://doi.org/10.3390/ijms21020419.
Chicago author-date (all authors)
Smits, Katrien, Yannick Gansemans, Laurentijn Tilleman, Filip Van Nieuwerburgh, Margot Van de Velde, Ilse Gerits, Cyrillus Ververs, Kim Roels, Jan Govaere, Luc Peelman, Dieter Deforce, and Ann Van Soom. 2020. “Maternal Recognition of Pregnancy in the Horse : Are MicroRNAs the Secret Messengers?” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 (2). doi:10.3390/ijms21020419.
Vancouver
1.
Smits K, Gansemans Y, Tilleman L, Van Nieuwerburgh F, Van de Velde M, Gerits I, et al. Maternal recognition of pregnancy in the horse : are MicroRNAs the secret messengers? INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2020;21(2).
IEEE
[1]
K. Smits et al., “Maternal recognition of pregnancy in the horse : are MicroRNAs the secret messengers?,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 2, 2020.
@article{8656574,
  abstract     = {{The signal for maternal recognition of pregnancy (MRP) has still not been identified in the horse. High-throughput molecular biology at the embryo-maternal interface has substantially contributed to the knowledge on pathways affected during MRP, but an integrated study in which proteomics, transcriptomics and miRNA expression can be linked directly is currently lacking. The aim of this study was to provide such analysis. Endometrial biopsies, uterine fluid, embryonic tissues, and yolk sac fluid were collected 13 days after ovulation during pregnant and control cycles from the same mares. Micro-RNA-Sequencing was performed on all collected samples, mRNA-Sequencing on the same tissue samples and mass spectrometry was conducted previously on the same fluid samples. Differential expression of miRNA, mRNA and proteins showed high conformity with literature and confirmed involvement in pregnancy establishment, embryo quality, steroid synthesis and prostaglandin regulation, but the link between differential miRNAs and their targets was limited and did not indicate the identity of an unequivocal signal for MRP in the horse. Differential expression at the embryo-maternal interface was prominent, highlighting a potential role of miRNAs in embryo-maternal communication during early pregnancy in the horse. These data provide a strong basis for future targeted studies.}},
  articleno    = {{419}},
  author       = {{Smits, Katrien and Gansemans, Yannick and Tilleman, Laurentijn and Van Nieuwerburgh, Filip and Van de Velde, Margot and Gerits, Ilse and Ververs, Cyrillus and Roels, Kim and Govaere, Jan and Peelman, Luc and Deforce, Dieter and Van Soom, Ann}},
  issn         = {{1422-0067}},
  journal      = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
  keywords     = {{Physical and Theoretical Chemistry,Inorganic Chemistry,Organic Chemistry,Spectroscopy,Molecular Biology,Catalysis,General Medicine,Computer Science Applications,maternal recognition of pregnancy,horse,RNA-Sequencing,proteomics,microRNA,EQUINE ENDOMETRIUM,ESTROUS-CYCLE,DIFFERENTIAL EXPRESSION,TROPHOBLASTIC CELLS,FOLLICULAR-FLUID,IMPLANTATION,OXYTOCIN,MIGRATION,INVASION,RECEPTOR}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{17}},
  title        = {{Maternal recognition of pregnancy in the horse : are MicroRNAs the secret messengers?}},
  url          = {{http://dx.doi.org/10.3390/ijms21020419}},
  volume       = {{21}},
  year         = {{2020}},
}

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