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Carbamoylated nail proteins as assessed by near-infrared analysis are associated with load of uremic toxins and mortality in hemodialysis patients

Sander De Bruyne (UGent) , Jonas Himpe (UGent) , Sigurd Delanghe (UGent) , Griet Glorieux (UGent) , Wim Van Biesen (UGent) , Marc De Buyzere (UGent) , Marijn Speeckaert (UGent) and Joris Delanghe (UGent)
(2020) TOXINS. 12(2).
Author
Organization
Abstract
Carbamoylation is an important risk factor for accelerated atherogenesis and mortality in patients undergoing hemodialysis (HD). We intended to explore whether carbamoylation as assessed by near-infrared (NIR) analysis of nail proteins is associated with (a) plasma concentrations of representative uremic toxins and (b) mortality in HD patients. A total of 53 healthy volunteers and 84 consecutive HD patients were enrolled in this cross-sectional cohort study. Standard laboratory methods were used to measure routine parameters, whereas levels of uremic toxins were determined using reversed-phase high-performance liquid chromatography (RP-HPLC). Spectra of distal fingernail clippings were obtained using an Avantes NIR spectrometer and processed using chemometric data analysis. The second derivative of the peak intensity at 1494 nm attributed to N-H amide bands from NH2 of carbamoyl (-CONH2) groups was higher in HD patients than in control subjects (p < 0.0001). Peak intensity levels were associated with age and plasma levels of representative uremic toxins. Cox-regression analysis revealed a significant association with all-cause mortality, even after adjustment for age. In conclusion, our data revealed that carbamoylation as assessed by NIR analysis of nail proteins is associated with plasma concentrations of uremic toxins and also with mortality in HD patients. Further research to explore whether it is a surrogate marker or a hard indicator of mortality risk is warranted.
Keywords
carbamoylation, hemodialysis, keratins, mortality, nails, near-infrared spectroscopy, uremia, uremic toxins, PERITONEAL-DIALYSIS PATIENTS, CARBAMYLATION, SPECTROSCOPY, COMORBIDITY, GLYCATION, MARKER

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MLA
De Bruyne, Sander, et al. “Carbamoylated Nail Proteins as Assessed by Near-Infrared Analysis Are Associated with Load of Uremic Toxins and Mortality in Hemodialysis Patients.” TOXINS, vol. 12, no. 2, 2020, doi:10.3390/toxins12020083.
APA
De Bruyne, S., Himpe, J., Delanghe, S., Glorieux, G., Van Biesen, W., De Buyzere, M., … Delanghe, J. (2020). Carbamoylated nail proteins as assessed by near-infrared analysis are associated with load of uremic toxins and mortality in hemodialysis patients. TOXINS, 12(2). https://doi.org/10.3390/toxins12020083
Chicago author-date
De Bruyne, Sander, Jonas Himpe, Sigurd Delanghe, Griet Glorieux, Wim Van Biesen, Marc De Buyzere, Marijn Speeckaert, and Joris Delanghe. 2020. “Carbamoylated Nail Proteins as Assessed by Near-Infrared Analysis Are Associated with Load of Uremic Toxins and Mortality in Hemodialysis Patients.” TOXINS 12 (2). https://doi.org/10.3390/toxins12020083.
Chicago author-date (all authors)
De Bruyne, Sander, Jonas Himpe, Sigurd Delanghe, Griet Glorieux, Wim Van Biesen, Marc De Buyzere, Marijn Speeckaert, and Joris Delanghe. 2020. “Carbamoylated Nail Proteins as Assessed by Near-Infrared Analysis Are Associated with Load of Uremic Toxins and Mortality in Hemodialysis Patients.” TOXINS 12 (2). doi:10.3390/toxins12020083.
Vancouver
1.
De Bruyne S, Himpe J, Delanghe S, Glorieux G, Van Biesen W, De Buyzere M, et al. Carbamoylated nail proteins as assessed by near-infrared analysis are associated with load of uremic toxins and mortality in hemodialysis patients. TOXINS. 2020;12(2).
IEEE
[1]
S. De Bruyne et al., “Carbamoylated nail proteins as assessed by near-infrared analysis are associated with load of uremic toxins and mortality in hemodialysis patients,” TOXINS, vol. 12, no. 2, 2020.
@article{8656464,
  abstract     = {{Carbamoylation is an important risk factor for accelerated atherogenesis and mortality in patients undergoing hemodialysis (HD). We intended to explore whether carbamoylation as assessed by near-infrared (NIR) analysis of nail proteins is associated with (a) plasma concentrations of representative uremic toxins and (b) mortality in HD patients. A total of 53 healthy volunteers and 84 consecutive HD patients were enrolled in this cross-sectional cohort study. Standard laboratory methods were used to measure routine parameters, whereas levels of uremic toxins were determined using reversed-phase high-performance liquid chromatography (RP-HPLC). Spectra of distal fingernail clippings were obtained using an Avantes NIR spectrometer and processed using chemometric data analysis. The second derivative of the peak intensity at 1494 nm attributed to N-H amide bands from NH2 of carbamoyl (-CONH2) groups was higher in HD patients than in control subjects (p < 0.0001). Peak intensity levels were associated with age and plasma levels of representative uremic toxins. Cox-regression analysis revealed a significant association with all-cause mortality, even after adjustment for age. In conclusion, our data revealed that carbamoylation as assessed by NIR analysis of nail proteins is associated with plasma concentrations of uremic toxins and also with mortality in HD patients. Further research to explore whether it is a surrogate marker or a hard indicator of mortality risk is warranted.}},
  articleno    = {{83}},
  author       = {{De Bruyne, Sander and Himpe, Jonas and Delanghe, Sigurd and Glorieux, Griet and Van Biesen, Wim and De Buyzere, Marc and Speeckaert, Marijn and Delanghe, Joris}},
  issn         = {{2072-6651}},
  journal      = {{TOXINS}},
  keywords     = {{carbamoylation,hemodialysis,keratins,mortality,nails,near-infrared spectroscopy,uremia,uremic toxins,PERITONEAL-DIALYSIS PATIENTS,CARBAMYLATION,SPECTROSCOPY,COMORBIDITY,GLYCATION,MARKER}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{13}},
  title        = {{Carbamoylated nail proteins as assessed by near-infrared analysis are associated with load of uremic toxins and mortality in hemodialysis patients}},
  url          = {{http://dx.doi.org/10.3390/toxins12020083}},
  volume       = {{12}},
  year         = {{2020}},
}

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