Advanced search
1 file | 16.11 KB Add to list

The role of epigenetics in the interaction between rice and parasitic nematodes

Author
Organization
Abstract
Rice is the most important staple food grain worldwide. The root-knot nematode Meloidogyne graminicola is one of its most destructive belowground pathogens, inducing the formation of root galls. Transcriptome analyses on galls have revealed major reprogramming and pointed to a role for epigenetic regulation. We are investigating the major epigenetic pathways in infected rice roots. First global DNA methylation assays, confirmed and extended by whole genome bisulfite sequencing, revealed major DNA hypomethylation of cytosine in the CHH context in galls. Mutants in the RNA-directed DNA methylation (RdDM) pathway, and azacitidine treatment revealed that loss of DNA methylation leads to reduced susceptibility. Long non-coding RNAs (lncRNAs) and small RNAs (smRNAs) are major players in RdDM. Using total RNA-sequencing, covering smRNAs as well as poly-adenylated and non-adenylated lncRNAs, we revealed more than 1000 differentially expressed non-coding rice transcripts that respond to nematode infection. Next to that, ChIP-seq showed major hypermethylation of histones in nematode-induced galls, consistent with strong transcriptional activation of histone lysine methyltransferases. Finally, transgenerational experiments revealed that offspring of nematode-infected rice plants are significantly less susceptible. These results demonstrate that epigenetic changes are a major regulator of rice defence against nematodes, and that they may even be inheritable.

Downloads

  • MPMIAbstract 2019.docx
    • full text (Accepted manuscript)
    • |
    • open access
    • |
    • Word
    • |
    • 16.11 KB

Citation

Please use this url to cite or link to this publication:

MLA
Kyndt, Tina, et al. “The Role of Epigenetics in the Interaction between Rice and Parasitic Nematodes.” MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 32, no. 10, 2019, pp. 142–142.
APA
Kyndt, T., Atighi Quchan Atigh, M., Verstraeten, B., Meijer, A., & De Meyer, T. (2019). The role of epigenetics in the interaction between rice and parasitic nematodes. In MOLECULAR PLANT-MICROBE INTERACTIONS (Vol. 32, pp. 142–142). Glasgow.
Chicago author-date
Kyndt, Tina, Mohammad Atighi Quchan Atigh, Bruno Verstraeten, Aniko Meijer, and Tim De Meyer. 2019. “The Role of Epigenetics in the Interaction between Rice and Parasitic Nematodes.” In MOLECULAR PLANT-MICROBE INTERACTIONS, 32:142–142.
Chicago author-date (all authors)
Kyndt, Tina, Mohammad Atighi Quchan Atigh, Bruno Verstraeten, Aniko Meijer, and Tim De Meyer. 2019. “The Role of Epigenetics in the Interaction between Rice and Parasitic Nematodes.” In MOLECULAR PLANT-MICROBE INTERACTIONS, 32:142–142.
Vancouver
1.
Kyndt T, Atighi Quchan Atigh M, Verstraeten B, Meijer A, De Meyer T. The role of epigenetics in the interaction between rice and parasitic nematodes. In: MOLECULAR PLANT-MICROBE INTERACTIONS. 2019. p. 142–142.
IEEE
[1]
T. Kyndt, M. Atighi Quchan Atigh, B. Verstraeten, A. Meijer, and T. De Meyer, “The role of epigenetics in the interaction between rice and parasitic nematodes,” in MOLECULAR PLANT-MICROBE INTERACTIONS, Glasgow, 2019, vol. 32, no. 10, pp. 142–142.
@inproceedings{8655753,
  abstract     = {{Rice is the most important staple food grain worldwide. The root-knot nematode Meloidogyne graminicola is one of its most destructive belowground pathogens, inducing the formation of root galls. Transcriptome analyses on galls have revealed major reprogramming and pointed to a role for epigenetic regulation. We are investigating the major epigenetic pathways in infected rice roots. First global DNA methylation assays, confirmed and extended by whole genome bisulfite sequencing, revealed major DNA hypomethylation of cytosine in the CHH context in galls. Mutants in the RNA-directed DNA methylation (RdDM) pathway, and azacitidine treatment revealed that loss of DNA methylation leads to reduced susceptibility. Long non-coding RNAs (lncRNAs) and small RNAs (smRNAs) are major players in RdDM. Using total RNA-sequencing, covering smRNAs as well as poly-adenylated and non-adenylated lncRNAs, we revealed more than 1000 differentially expressed non-coding rice transcripts that respond to nematode infection. Next to that, ChIP-seq showed major hypermethylation of histones in nematode-induced galls, consistent with strong transcriptional activation of histone lysine methyltransferases. Finally, transgenerational experiments revealed that offspring of nematode-infected rice plants are significantly less susceptible. These results demonstrate that epigenetic changes are a major regulator of rice defence against nematodes, and that they may even be inheritable.}},
  author       = {{Kyndt, Tina and Atighi Quchan Atigh, Mohammad and Verstraeten, Bruno and Meijer, Aniko and De Meyer, Tim}},
  booktitle    = {{MOLECULAR PLANT-MICROBE INTERACTIONS}},
  issn         = {{0894-0282}},
  language     = {{eng}},
  location     = {{Glasgow}},
  number       = {{10}},
  pages        = {{142--142}},
  title        = {{The role of epigenetics in the interaction between rice and parasitic nematodes}},
  volume       = {{32}},
  year         = {{2019}},
}

Web of Science
Times cited: