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The use of PTI-marker genes to identify novel compounds that establish induced resistance in rice

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Abstract
Compounds that establish induced resistance (IR) in plants are promising alternatives for the pesticides that are progressively being banned worldwide. Screening platforms to identify IR-establishing compounds have been developed, but none were specifically designed for monocot plants. Here, we propose the use of an RT-qPCR screening platform, based on conserved immunity marker genes of rice as proxy for IR induction. Central regulators of biotic stress responses of rice were identified with a weighted gene co-expression network analysis (WGCNA), using more than 350 microarray datasets of rice under various sorts of biotic stress. Candidate genes were narrowed down to six immunity marker genes, based on consistent association with pattern-triggered immunity (PTI), both in rice plants as in rice cell suspension cultures (RCSCs). By monitoring the expression of these genes in RCSCs upon treatment with candidate IR-inducing compounds, we showed that our marker genes can predict IR induction in rice. Diproline, a novel IR-establishing compound for monocots that was detected with these marker genes, was shown to induce rice resistance against root-knot nematodes, without fitness costs. Gene expression profiling of the here-described PTI-marker genes can be executed on fully-grown plants or in RCSCs, providing a novel and versatile tool to predict IR induction.
Keywords
Physical and Theoretical Chemistry, Inorganic Chemistry, Organic Chemistry, Spectroscopy, Molecular Biology, Catalysis, General Medicine, Computer Science Applications, induced resistance, nematode-rice interactions, WGCNA, rice cell suspension cultures, PATTERN-TRIGGERED IMMUNITY, ROOT-KNOT NEMATODES, DISEASE RESISTANCE, DEFENSE, CHALLENGES, DATABASE, PACKAGE, TARGET

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MLA
De Kesel, Jonas, et al. “The Use of PTI-Marker Genes to Identify Novel Compounds That Establish Induced Resistance in Rice.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 1, 2020, doi:10.3390/ijms21010317.
APA
De Kesel, J., Gómez Rodríguez, R., Bonneure, E., Mangelinckx, S., & Kyndt, T. (2020). The use of PTI-marker genes to identify novel compounds that establish induced resistance in rice. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(1). https://doi.org/10.3390/ijms21010317
Chicago author-date
De Kesel, Jonas, Ramsés Gómez Rodríguez, Eli Bonneure, Sven Mangelinckx, and Tina Kyndt. 2020. “The Use of PTI-Marker Genes to Identify Novel Compounds That Establish Induced Resistance in Rice.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 (1). https://doi.org/10.3390/ijms21010317.
Chicago author-date (all authors)
De Kesel, Jonas, Ramsés Gómez Rodríguez, Eli Bonneure, Sven Mangelinckx, and Tina Kyndt. 2020. “The Use of PTI-Marker Genes to Identify Novel Compounds That Establish Induced Resistance in Rice.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 (1). doi:10.3390/ijms21010317.
Vancouver
1.
De Kesel J, Gómez Rodríguez R, Bonneure E, Mangelinckx S, Kyndt T. The use of PTI-marker genes to identify novel compounds that establish induced resistance in rice. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2020;21(1).
IEEE
[1]
J. De Kesel, R. Gómez Rodríguez, E. Bonneure, S. Mangelinckx, and T. Kyndt, “The use of PTI-marker genes to identify novel compounds that establish induced resistance in rice,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 1, 2020.
@article{8655748,
  abstract     = {{Compounds that establish induced resistance (IR) in plants are promising alternatives for the pesticides that are progressively being banned worldwide. Screening platforms to identify IR-establishing compounds have been developed, but none were specifically designed for monocot plants. Here, we propose the use of an RT-qPCR screening platform, based on conserved immunity marker genes of rice as proxy for IR induction. Central regulators of biotic stress responses of rice were identified with a weighted gene co-expression network analysis (WGCNA), using more than 350 microarray datasets of rice under various sorts of biotic stress. Candidate genes were narrowed down to six immunity marker genes, based on consistent association with pattern-triggered immunity (PTI), both in rice plants as in rice cell suspension cultures (RCSCs). By monitoring the expression of these genes in RCSCs upon treatment with candidate IR-inducing compounds, we showed that our marker genes can predict IR induction in rice. Diproline, a novel IR-establishing compound for monocots that was detected with these marker genes, was shown to induce rice resistance against root-knot nematodes, without fitness costs. Gene expression profiling of the here-described PTI-marker genes can be executed on fully-grown plants or in RCSCs, providing a novel and versatile tool to predict IR induction.}},
  articleno    = {{317}},
  author       = {{De Kesel, Jonas and Gómez Rodríguez, Ramsés and Bonneure, Eli and Mangelinckx, Sven and Kyndt, Tina}},
  issn         = {{1422-0067}},
  journal      = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
  keywords     = {{Physical and Theoretical Chemistry,Inorganic Chemistry,Organic Chemistry,Spectroscopy,Molecular Biology,Catalysis,General Medicine,Computer Science Applications,induced resistance,nematode-rice interactions,WGCNA,rice cell suspension cultures,PATTERN-TRIGGERED IMMUNITY,ROOT-KNOT NEMATODES,DISEASE RESISTANCE,DEFENSE,CHALLENGES,DATABASE,PACKAGE,TARGET}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{16}},
  title        = {{The use of PTI-marker genes to identify novel compounds that establish induced resistance in rice}},
  url          = {{http://dx.doi.org/10.3390/ijms21010317}},
  volume       = {{21}},
  year         = {{2020}},
}

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