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Implementation of HPLC analysis of γ-aminobutyric acid (GABA) in fermented food matrices

Phuöng Hong Lê (UGent) , Liesa Verscheure (UGent) , Thien Trung Le, Yannick Verheust (UGent) and Katleen Raes (UGent)
(2020) FOOD ANALYTICAL METHODS. 13(5). p.1190-1201
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Abstract
In this study, a high-performance liquid chromatography (HPLC) method was adapted to measure the gamma-aminobutyric acid (GABA) and glutamate (Glu) content in different fermented foods. The best solvent to extract GABA and Glu from the food matrices was 75% EtOH and water, combined with 4% SSA as posttreatment. The extracted amino acids were derivatized with o-phthalaldehyde (OPA) for 2 min at room temperature and analyzed by HPLC with fluorescence detector (lambda ex = 340 nm and lambda em = 455 nm). Animal-based fermented products had a higher GABA content, compared with plant-based and soy-based fermented products. Among 17 fermented samples, fish sauce showed the highest amount of GABA (16.29 +/- 0.53 mg/kg DW). Results obtained by the HPLC method were compared with those obtained by a spectrophotometric method, which is based on the reaction of omega-amino acids with phenol. All measured GABA contents, obtained by HPLC method, were much lower than those obtained by the spectrophotometric method, which was due to the nonspecific reaction of the coloring reagent with other amino acids than GABA. It can be concluded that GABA content in food products can only be measured by HPLC.
Keywords
Derivatization reagent, Extraction, Fermented foods, GABA, Glutamate, HPLC, PERFORMANCE LIQUID-CHROMATOGRAPHY, PHTHALDIALDEHYDE AMINO-ACID, PU-ERH TEAS, ORTHO-PHTHALALDEHYDE, BROWN RICE, STABILITY, GLUTAMATE, DERIVATIZATION, DERIVATIVES, BLACK

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MLA
Lê, Phuöng Hong, et al. “Implementation of HPLC Analysis of γ-Aminobutyric Acid (GABA) in Fermented Food Matrices.” FOOD ANALYTICAL METHODS, vol. 13, no. 5, 2020, pp. 1190–201, doi:10.1007/s12161-020-01734-2.
APA
Lê, P. H., Verscheure, L., Le, T. T., Verheust, Y., & Raes, K. (2020). Implementation of HPLC analysis of γ-aminobutyric acid (GABA) in fermented food matrices. FOOD ANALYTICAL METHODS, 13(5), 1190–1201. https://doi.org/10.1007/s12161-020-01734-2
Chicago author-date
Lê, Phuöng Hong, Liesa Verscheure, Thien Trung Le, Yannick Verheust, and Katleen Raes. 2020. “Implementation of HPLC Analysis of γ-Aminobutyric Acid (GABA) in Fermented Food Matrices.” FOOD ANALYTICAL METHODS 13 (5): 1190–1201. https://doi.org/10.1007/s12161-020-01734-2.
Chicago author-date (all authors)
Lê, Phuöng Hong, Liesa Verscheure, Thien Trung Le, Yannick Verheust, and Katleen Raes. 2020. “Implementation of HPLC Analysis of γ-Aminobutyric Acid (GABA) in Fermented Food Matrices.” FOOD ANALYTICAL METHODS 13 (5): 1190–1201. doi:10.1007/s12161-020-01734-2.
Vancouver
1.
Lê PH, Verscheure L, Le TT, Verheust Y, Raes K. Implementation of HPLC analysis of γ-aminobutyric acid (GABA) in fermented food matrices. FOOD ANALYTICAL METHODS. 2020;13(5):1190–201.
IEEE
[1]
P. H. Lê, L. Verscheure, T. T. Le, Y. Verheust, and K. Raes, “Implementation of HPLC analysis of γ-aminobutyric acid (GABA) in fermented food matrices,” FOOD ANALYTICAL METHODS, vol. 13, no. 5, pp. 1190–1201, 2020.
@article{8653844,
  abstract     = {In this study, a high-performance liquid chromatography (HPLC) method was adapted to measure the gamma-aminobutyric acid (GABA) and glutamate (Glu) content in different fermented foods. The best solvent to extract GABA and Glu from the food matrices was 75% EtOH and water, combined with 4% SSA as posttreatment. The extracted amino acids were derivatized with o-phthalaldehyde (OPA) for 2 min at room temperature and analyzed by HPLC with fluorescence detector (lambda ex = 340 nm and lambda em = 455 nm). Animal-based fermented products had a higher GABA content, compared with plant-based and soy-based fermented products. Among 17 fermented samples, fish sauce showed the highest amount of GABA (16.29 +/- 0.53 mg/kg DW). Results obtained by the HPLC method were compared with those obtained by a spectrophotometric method, which is based on the reaction of omega-amino acids with phenol. All measured GABA contents, obtained by HPLC method, were much lower than those obtained by the spectrophotometric method, which was due to the nonspecific reaction of the coloring reagent with other amino acids than GABA. It can be concluded that GABA content in food products can only be measured by HPLC.},
  author       = {Lê, Phuöng Hong and Verscheure, Liesa and Le, Thien Trung and Verheust, Yannick and Raes, Katleen},
  issn         = {1936-9751},
  journal      = {FOOD ANALYTICAL METHODS},
  keywords     = {Derivatization reagent,Extraction,Fermented foods,GABA,Glutamate,HPLC,PERFORMANCE LIQUID-CHROMATOGRAPHY,PHTHALDIALDEHYDE AMINO-ACID,PU-ERH TEAS,ORTHO-PHTHALALDEHYDE,BROWN RICE,STABILITY,GLUTAMATE,DERIVATIZATION,DERIVATIVES,BLACK},
  language     = {eng},
  number       = {5},
  pages        = {1190--1201},
  title        = {Implementation of HPLC analysis of γ-aminobutyric acid (GABA) in fermented food matrices},
  url          = {http://dx.doi.org/10.1007/s12161-020-01734-2},
  volume       = {13},
  year         = {2020},
}
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