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Permeability of membranes in the liquid ordered and liquid disordered phases

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Abstract
The functional significance of ordered nanodomains (or rafts) in cholesterol rich eukaryotic cell membranes has only begun to be explored. This study exploits the correspondence of cellular rafts and liquid ordered (L-o) phases of three-component lipid bilayers to examine permeability. Molecular dynamics simulations of L-o phase dipalmitoylphosphatidylcholine (DPPC), dioleoylphosphatidylcholine (DOPC), and cholesterol show that oxygen and water transit a leaflet through the DOPC and cholesterol rich boundaries of hexagonally packed DPPC microdomains, freely diffuse along the bilayer midplane, and escape the membrane along the boundary regions. Electron paramagnetic resonance experiments provide critical validation: the measured ratio of oxygen concentrations near the midplanes of liquid disordered (L-d) and L-o bilayers of DPPC/DOPC/cholesterol is 1.75 +/- 0.35, in very good agreement with 1.3 +/- 0.3 obtained from simulation. The results show how cellular rafts can be structurally rigid signaling platforms while remaining nearly as permeable to small molecules as the L-d phase.
Keywords
General Biochemistry, Genetics and Molecular Biology, General Physics and Astronomy, General Chemistry, OXYGEN PERMEABILITY, DYNAMICS SIMULATIONS, LIPID-MEMBRANES, DIFFUSION, CHOLESTEROL, PERMEATION, TEMPERATURE, BILAYERS, DOMAINS, RAFTS

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MLA
Ghysels, An, et al. “Permeability of Membranes in the Liquid Ordered and Liquid Disordered Phases.” NATURE COMMUNICATIONS, vol. 10, 2019, doi:10.1038/s41467-019-13432-7.
APA
Ghysels, A., Krämer, A., Venable, R. M., Teague, W. E., Lyman, E., Gawrisch, K., & Pastor, R. W. (2019). Permeability of membranes in the liquid ordered and liquid disordered phases. NATURE COMMUNICATIONS, 10. https://doi.org/10.1038/s41467-019-13432-7
Chicago author-date
Ghysels, An, Andreas Krämer, Richard M. Venable, Walter E. Teague, Edward Lyman, Klaus Gawrisch, and Richard W. Pastor. 2019. “Permeability of Membranes in the Liquid Ordered and Liquid Disordered Phases.” NATURE COMMUNICATIONS 10. https://doi.org/10.1038/s41467-019-13432-7.
Chicago author-date (all authors)
Ghysels, An, Andreas Krämer, Richard M. Venable, Walter E. Teague, Edward Lyman, Klaus Gawrisch, and Richard W. Pastor. 2019. “Permeability of Membranes in the Liquid Ordered and Liquid Disordered Phases.” NATURE COMMUNICATIONS 10. doi:10.1038/s41467-019-13432-7.
Vancouver
1.
Ghysels A, Krämer A, Venable RM, Teague WE, Lyman E, Gawrisch K, et al. Permeability of membranes in the liquid ordered and liquid disordered phases. NATURE COMMUNICATIONS. 2019;10.
IEEE
[1]
A. Ghysels et al., “Permeability of membranes in the liquid ordered and liquid disordered phases,” NATURE COMMUNICATIONS, vol. 10, 2019.
@article{8652547,
  abstract     = {{The functional significance of ordered nanodomains (or rafts) in cholesterol rich eukaryotic cell membranes has only begun to be explored. This study exploits the correspondence of cellular rafts and liquid ordered (L-o) phases of three-component lipid bilayers to examine permeability. Molecular dynamics simulations of L-o phase dipalmitoylphosphatidylcholine (DPPC), dioleoylphosphatidylcholine (DOPC), and cholesterol show that oxygen and water transit a leaflet through the DOPC and cholesterol rich boundaries of hexagonally packed DPPC microdomains, freely diffuse along the bilayer midplane, and escape the membrane along the boundary regions. Electron paramagnetic resonance experiments provide critical validation: the measured ratio of oxygen concentrations near the midplanes of liquid disordered (L-d) and L-o bilayers of DPPC/DOPC/cholesterol is 1.75 +/- 0.35, in very good agreement with 1.3 +/- 0.3 obtained from simulation. The results show how cellular rafts can be structurally rigid signaling platforms while remaining nearly as permeable to small molecules as the L-d phase.}},
  articleno    = {{5616}},
  author       = {{Ghysels, An and Krämer, Andreas and Venable, Richard M. and Teague, Walter E. and Lyman, Edward and Gawrisch, Klaus and Pastor, Richard W.}},
  issn         = {{2041-1723}},
  journal      = {{NATURE COMMUNICATIONS}},
  keywords     = {{General Biochemistry,Genetics and Molecular Biology,General Physics and Astronomy,General Chemistry,OXYGEN PERMEABILITY,DYNAMICS SIMULATIONS,LIPID-MEMBRANES,DIFFUSION,CHOLESTEROL,PERMEATION,TEMPERATURE,BILAYERS,DOMAINS,RAFTS}},
  language     = {{eng}},
  pages        = {{12}},
  title        = {{Permeability of membranes in the liquid ordered and liquid disordered phases}},
  url          = {{http://doi.org/10.1038/s41467-019-13432-7}},
  volume       = {{10}},
  year         = {{2019}},
}

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