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The use of selected bacteria and yeasts to control vibrio spp. in live food

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Abstract
Vibrio species are a significant causative of mass mortality in mariculture worldwide, which can quickly accumulate in live food and transmit into the larval gut. With restrictions on the use of antibiotics in aquaculture, finding a proper solution to reduce the risk of Vibriosis is vital. This study aimed to evaluate the susceptibility of Vibrio harveyi, V. campbellii, V. anguillarum, and V. parahaemolyticus to twenty-six bacterial and yeast strains and use the beneficial ones to enrich live food (Branchiopod, Artemia franciscana, rotifer, Brachionus plicatilis and copepod, Tigriopus japonicus). Thus, a modified disk diffusion method was applied. After a susceptibility assay, the bacteria and yeast beneficial in suppressing the Vibrio species were labeled by fluorescent stain and used to measure the accumulation potential in different live foods. Also, the beneficial bacteria and yeast were used to enrich live foods, and then the count of loaded Vibrio was estimated after 5, 10, 15, and 20 h by the serial dilution method. From the total bacteria and yeast strains that were used, Candida parapsilosis, Pseudoalteromonas flavipulchra, Lactobacillus sakei, Bacillus natto, and B. amyloliquefaciens inhibited all four Vibrio species. The results of microbial labeling showed that L. sakei in Artemia, C. parapsilosis in rotifers, and V. harveyi in copepods had the highest accumulation rate. The results of the estimation of loaded Vibrio in different live foods also showed that the use of beneficial bacteria and yeast each significantly reduced the count of Vibrio. Application of bacteria and yeast to suppress pathogenic Vibrio maybe a sustainable method for preventing this pathogen from harmfully invading aquaculture and may also aid in reducing the chances of antibiotic resistance in pathogenic Vibrio.
Keywords
General Pharmacology, Toxicology and Pharmaceutics, Microbiology (medical), Biochemistry, Pharmacology (medical), Microbiology, Infectious Diseases, aquaculture, bacillus, lactobacillus, yeasts, resistance, Vibrio, GROWTH-PERFORMANCE, ANTIBIOTIC SUSCEPTIBILITY, ANTIMICROBIAL ACTIVITY, BRACHIONUS-PLICATILIS, ARTEMIA-FRANCISCANA, PENAEUS-MONODON, BRINE SHRIMP, LACTOBACILLUS, FISH, PARAHAEMOLYTICUS

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MLA
Sahandi, Javad, et al. “The Use of Selected Bacteria and Yeasts to Control Vibrio Spp. in Live Food.” ANTIBIOTICS-BASEL, vol. 8, no. 3, 2019, doi:10.3390/antibiotics8030095.
APA
Sahandi, J., Sorgeloos, P., Xiao, H., Wang, X., Qi, Z., Zheng, Y., & Tang, X. (2019). The use of selected bacteria and yeasts to control vibrio spp. in live food. ANTIBIOTICS-BASEL, 8(3). https://doi.org/10.3390/antibiotics8030095
Chicago author-date
Sahandi, Javad, Patrick Sorgeloos, Hui Xiao, Xianghong Wang, Zizhong Qi, Yanfen Zheng, and Xuexi Tang. 2019. “The Use of Selected Bacteria and Yeasts to Control Vibrio Spp. in Live Food.” ANTIBIOTICS-BASEL 8 (3). https://doi.org/10.3390/antibiotics8030095.
Chicago author-date (all authors)
Sahandi, Javad, Patrick Sorgeloos, Hui Xiao, Xianghong Wang, Zizhong Qi, Yanfen Zheng, and Xuexi Tang. 2019. “The Use of Selected Bacteria and Yeasts to Control Vibrio Spp. in Live Food.” ANTIBIOTICS-BASEL 8 (3). doi:10.3390/antibiotics8030095.
Vancouver
1.
Sahandi J, Sorgeloos P, Xiao H, Wang X, Qi Z, Zheng Y, et al. The use of selected bacteria and yeasts to control vibrio spp. in live food. ANTIBIOTICS-BASEL. 2019;8(3).
IEEE
[1]
J. Sahandi et al., “The use of selected bacteria and yeasts to control vibrio spp. in live food,” ANTIBIOTICS-BASEL, vol. 8, no. 3, 2019.
@article{8650721,
  abstract     = {{Vibrio species are a significant causative of mass mortality in mariculture worldwide, which can quickly accumulate in live food and transmit into the larval gut. With restrictions on the use of antibiotics in aquaculture, finding a proper solution to reduce the risk of Vibriosis is vital. This study aimed to evaluate the susceptibility of Vibrio harveyi, V. campbellii, V. anguillarum, and V. parahaemolyticus to twenty-six bacterial and yeast strains and use the beneficial ones to enrich live food (Branchiopod, Artemia franciscana, rotifer, Brachionus plicatilis and copepod, Tigriopus japonicus). Thus, a modified disk diffusion method was applied. After a susceptibility assay, the bacteria and yeast beneficial in suppressing the Vibrio species were labeled by fluorescent stain and used to measure the accumulation potential in different live foods. Also, the beneficial bacteria and yeast were used to enrich live foods, and then the count of loaded Vibrio was estimated after 5, 10, 15, and 20 h by the serial dilution method. From the total bacteria and yeast strains that were used, Candida parapsilosis, Pseudoalteromonas flavipulchra, Lactobacillus sakei, Bacillus natto, and B. amyloliquefaciens inhibited all four Vibrio species. The results of microbial labeling showed that L. sakei in Artemia, C. parapsilosis in rotifers, and V. harveyi in copepods had the highest accumulation rate. The results of the estimation of loaded Vibrio in different live foods also showed that the use of beneficial bacteria and yeast each significantly reduced the count of Vibrio. Application of bacteria and yeast to suppress pathogenic Vibrio maybe a sustainable method for preventing this pathogen from harmfully invading aquaculture and may also aid in reducing the chances of antibiotic resistance in pathogenic Vibrio.}},
  articleno    = {{95}},
  author       = {{Sahandi, Javad and Sorgeloos, Patrick and Xiao, Hui and Wang, Xianghong and Qi, Zizhong and Zheng, Yanfen and Tang, Xuexi}},
  issn         = {{2079-6382}},
  journal      = {{ANTIBIOTICS-BASEL}},
  keywords     = {{General Pharmacology,Toxicology and Pharmaceutics,Microbiology (medical),Biochemistry,Pharmacology (medical),Microbiology,Infectious Diseases,aquaculture,bacillus,lactobacillus,yeasts,resistance,Vibrio,GROWTH-PERFORMANCE,ANTIBIOTIC SUSCEPTIBILITY,ANTIMICROBIAL ACTIVITY,BRACHIONUS-PLICATILIS,ARTEMIA-FRANCISCANA,PENAEUS-MONODON,BRINE SHRIMP,LACTOBACILLUS,FISH,PARAHAEMOLYTICUS}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{16}},
  title        = {{The use of selected bacteria and yeasts to control vibrio spp. in live food}},
  url          = {{http://doi.org/10.3390/antibiotics8030095}},
  volume       = {{8}},
  year         = {{2019}},
}

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