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Immune and bacterial toxin genes expression in different giant tiger prawn, Penaeus monodon post-larvae stages following AHPND-causing strain of Vibrio parahaemolyticus challenge

Zulaikha Mat Deris, Shumpei Iehata, Mhd Ikhwanuddin, Mohd Badrul Mohamad Khairul Sahimi, Thinh Dinh Do, Patrick Sorgeloos (UGent) , Yeong Yik Sung and Li Lian Wong
(2020) AQUACULTURE REPORTS. 16.
Author
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Abstract
Acute Hepatopancreatic Necrosis Disease (AHPND), a disease caused by Vibrio parahaemolyticus (V-PAHPND), kills Penaeid shrimps worldwide, resulting in severe economic losses during aquaculture. To further understand how Penaeus monodon post-larvae (PL) respond towards infection of this pathogenic bacterium, the expression of several important immune and bacterial toxin genes in three stages of P. monodon PL (PL15, PL30 and PL45) upon V-PAHPND challenge, were determined. A 20-hrs challenge test with 2.7 x 10(7) cfu ml(-1) of V-PADPND resulted 81, 65 and 1.7% mortality respectively for PL30, PL15 and PL45, indicating that PL30 was most vulnerable to V-PADPNEP The immune response of shrimp PL at this stage was robust, with Toll-like receptor (TLR), prophenoloxidase (proPO), lysozyme (lyso) and penaeidin (PEN) augmented approximately 10.7, 4.7, 6.5 and 3.2-fold, respectively. The expression initiated at one hour post-infection (h.p.i), peaked at 16 h.p.i and 20 h.p.i, and decreased at 18 h.p.i, indicating the crucial involvement of these immune related genes in the defence and recovery of the first-line defence mechanisms during V-PADPND infection. This work also revealed that toxR gene represents a good indicator gene for Vibrio detection whereas PIR A, for V-PADPND pathogenicity determination of P. monodon. Overall, these findings provided novel insights into the immune response and V-PADPND No susceptibility of different P. monodon PL stages during infection, with outcomes potentially useful in enhancing the application of health therapy and biosecured aquaculture practices to minimize the damaging risk of AHPND towards sustainable production of P. monodon.
Keywords
Penaeus monodon, AHPND, PIR a, Immunity genes, Bacterial toxin gene, HEPATOPANCREATIC NECROSIS DISEASE, PROPHENOLOXIDASE-ACTIVATING SYSTEM, FRESH-WATER CRAYFISH, ANTIMICROBIAL PEPTIDES, LITOPENAEUS-VANNAMEI, KURUMA SHRIMP, LYSOZYME GENE, WHITE SHRIMP, RESPONSES, RECEPTOR

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MLA
Deris, Zulaikha Mat, et al. “Immune and Bacterial Toxin Genes Expression in Different Giant Tiger Prawn, Penaeus Monodon Post-Larvae Stages Following AHPND-Causing Strain of Vibrio Parahaemolyticus Challenge.” AQUACULTURE REPORTS, vol. 16, 2020, doi:10.1016/j.aqrep.2019.100248.
APA
Deris, Z. M., Iehata, S., Ikhwanuddin, M., Sahimi, M. B. M. K., Dinh Do, T., Sorgeloos, P., … Wong, L. L. (2020). Immune and bacterial toxin genes expression in different giant tiger prawn, Penaeus monodon post-larvae stages following AHPND-causing strain of Vibrio parahaemolyticus challenge. AQUACULTURE REPORTS, 16. https://doi.org/10.1016/j.aqrep.2019.100248
Chicago author-date
Deris, Zulaikha Mat, Shumpei Iehata, Mhd Ikhwanuddin, Mohd Badrul Mohamad Khairul Sahimi, Thinh Dinh Do, Patrick Sorgeloos, Yeong Yik Sung, and Li Lian Wong. 2020. “Immune and Bacterial Toxin Genes Expression in Different Giant Tiger Prawn, Penaeus Monodon Post-Larvae Stages Following AHPND-Causing Strain of Vibrio Parahaemolyticus Challenge.” AQUACULTURE REPORTS 16. https://doi.org/10.1016/j.aqrep.2019.100248.
Chicago author-date (all authors)
Deris, Zulaikha Mat, Shumpei Iehata, Mhd Ikhwanuddin, Mohd Badrul Mohamad Khairul Sahimi, Thinh Dinh Do, Patrick Sorgeloos, Yeong Yik Sung, and Li Lian Wong. 2020. “Immune and Bacterial Toxin Genes Expression in Different Giant Tiger Prawn, Penaeus Monodon Post-Larvae Stages Following AHPND-Causing Strain of Vibrio Parahaemolyticus Challenge.” AQUACULTURE REPORTS 16. doi:10.1016/j.aqrep.2019.100248.
Vancouver
1.
Deris ZM, Iehata S, Ikhwanuddin M, Sahimi MBMK, Dinh Do T, Sorgeloos P, et al. Immune and bacterial toxin genes expression in different giant tiger prawn, Penaeus monodon post-larvae stages following AHPND-causing strain of Vibrio parahaemolyticus challenge. AQUACULTURE REPORTS. 2020;16.
IEEE
[1]
Z. M. Deris et al., “Immune and bacterial toxin genes expression in different giant tiger prawn, Penaeus monodon post-larvae stages following AHPND-causing strain of Vibrio parahaemolyticus challenge,” AQUACULTURE REPORTS, vol. 16, 2020.
@article{8650719,
  abstract     = {{Acute Hepatopancreatic Necrosis Disease (AHPND), a disease caused by Vibrio parahaemolyticus (V-PAHPND), kills Penaeid shrimps worldwide, resulting in severe economic losses during aquaculture. To further understand how Penaeus monodon post-larvae (PL) respond towards infection of this pathogenic bacterium, the expression of several important immune and bacterial toxin genes in three stages of P. monodon PL (PL15, PL30 and PL45) upon V-PAHPND challenge, were determined. A 20-hrs challenge test with 2.7 x 10(7) cfu ml(-1) of V-PADPND resulted 81, 65 and 1.7% mortality respectively for PL30, PL15 and PL45, indicating that PL30 was most vulnerable to V-PADPNEP The immune response of shrimp PL at this stage was robust, with Toll-like receptor (TLR), prophenoloxidase (proPO), lysozyme (lyso) and penaeidin (PEN) augmented approximately 10.7, 4.7, 6.5 and 3.2-fold, respectively. The expression initiated at one hour post-infection (h.p.i), peaked at 16 h.p.i and 20 h.p.i, and decreased at 18 h.p.i, indicating the crucial involvement of these immune related genes in the defence and recovery of the first-line defence mechanisms during V-PADPND infection. This work also revealed that toxR gene represents a good indicator gene for Vibrio detection whereas PIR A, for V-PADPND pathogenicity determination of P. monodon. Overall, these findings provided novel insights into the immune response and V-PADPND No susceptibility of different P. monodon PL stages during infection, with outcomes potentially useful in enhancing the application of health therapy and biosecured aquaculture practices to minimize the damaging risk of AHPND towards sustainable production of P. monodon.}},
  articleno    = {{100248}},
  author       = {{Deris, Zulaikha Mat and Iehata, Shumpei and Ikhwanuddin, Mhd and Sahimi, Mohd Badrul Mohamad Khairul and Dinh Do, Thinh and Sorgeloos, Patrick and Sung, Yeong Yik and Wong, Li Lian}},
  issn         = {{2352-5134}},
  journal      = {{AQUACULTURE REPORTS}},
  keywords     = {{Penaeus monodon,AHPND,PIR a,Immunity genes,Bacterial toxin gene,HEPATOPANCREATIC NECROSIS DISEASE,PROPHENOLOXIDASE-ACTIVATING SYSTEM,FRESH-WATER CRAYFISH,ANTIMICROBIAL PEPTIDES,LITOPENAEUS-VANNAMEI,KURUMA SHRIMP,LYSOZYME GENE,WHITE SHRIMP,RESPONSES,RECEPTOR}},
  language     = {{eng}},
  pages        = {{7}},
  title        = {{Immune and bacterial toxin genes expression in different giant tiger prawn, Penaeus monodon post-larvae stages following AHPND-causing strain of Vibrio parahaemolyticus challenge}},
  url          = {{http://doi.org/10.1016/j.aqrep.2019.100248}},
  volume       = {{16}},
  year         = {{2020}},
}
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