Advanced search
1 file | 1.23 MB Add to list

RNA interference of Hsp70 in Artemia franciscana nauplii and its effect on morphology, growth, survival and immune response

(2020) AQUACULTURE. 520.
Author
Organization
Abstract
Brine shrimp Artemia franciscana release either encysted embryos via oviparous reproduction or motile nauplii via ovoviviparous reproduction. Artemia cysts contain an abundant amount of ArHsp21, ArHsp22, artemin, and p26, small heat shock proteins which regulate diapause and stress tolerance of this branchiopod crustacean. However, not much is known of the role of molecular chaperone, Hsp70 in this animal. In this study, the immunostimulatory role of Hsp70 in A. franciscana was investigated, work which included examining the function of this protein in embryo development, growth, survival and stress tolerance. Knockdown of Hsp70 was successfully performed by RNA interference (RNAi), with specific Hsp70 dsRNA construct delivered to female broods by microinjection to knockdown the protein in nauplii. The loss of Hsp70 neither affected the growth nor morphology as Artemia nauplii hatched normally and thereafter grew to adult within 28 days, observations similar to the control animals, the latter injected with GFP dsRNA. The survival of nauplii lacking Hsp70 appeared lower upon 28 days culture but the differences were insignificant when comparisons were made with the controls (P > .05). On other aspect, knockdown of Hsp70 reduced approximately 31% survival upon Vibrio campbellii challenge, indicating that Hsp70 assisted in the tolerance of Artemia nauplii during pathogen infection, perhaps through immune stimulation. Initial findings revealed that Hsp70 knockdown did not affect the expression of prophenoloxidase (proPO), a crucial immune protein of the shrimp innate immunity. By examining the morphology, growth, viability and molecular changes upon Hsp70 elimination, this study contributes to our understanding of the important roles of Hsp70 in A. franciscana, an important live food species used in aquaculture.
Keywords
Hsp70, Artemia franciscana, Nauplii, RNA interference (RNAi), HEAT-SHOCK PROTEINS, PROPHENOLOXIDASE SYSTEM, TOLERANCE, LARVAE, GENES, DIFFERENTIATION, RESISTANCE, KNOCKDOWN, DIAPAUSE, PROTECTS

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.23 MB

Citation

Please use this url to cite or link to this publication:

MLA
Iryani, Mat Taib Mimi, et al. “RNA Interference of Hsp70 in Artemia Franciscana Nauplii and Its Effect on Morphology, Growth, Survival and Immune Response.” AQUACULTURE, vol. 520, 2020, doi:10.1016/j.aquaculture.2020.735012.
APA
Iryani, M. T. M., MacRae, T. H., Sorgeloos, P., Muhammad, T. S. T., Danish-Daniel, M., Tan, M. P., … Sung, Y. Y. (2020). RNA interference of Hsp70 in Artemia franciscana nauplii and its effect on morphology, growth, survival and immune response. AQUACULTURE, 520. https://doi.org/10.1016/j.aquaculture.2020.735012
Chicago author-date
Iryani, Mat Taib Mimi, Thomas H MacRae, Patrick Sorgeloos, Tengku Sifzizul Tengku Muhammad, Muhd Danish-Daniel, Min Pau Tan, Woro Hastuti Satyantini, et al. 2020. “RNA Interference of Hsp70 in Artemia Franciscana Nauplii and Its Effect on Morphology, Growth, Survival and Immune Response.” AQUACULTURE 520. https://doi.org/10.1016/j.aquaculture.2020.735012.
Chicago author-date (all authors)
Iryani, Mat Taib Mimi, Thomas H MacRae, Patrick Sorgeloos, Tengku Sifzizul Tengku Muhammad, Muhd Danish-Daniel, Min Pau Tan, Woro Hastuti Satyantini, Mohd Effendy Abd Wahid, Jingfeng Sun, Aijun Lv, and Yeong Yik Sung. 2020. “RNA Interference of Hsp70 in Artemia Franciscana Nauplii and Its Effect on Morphology, Growth, Survival and Immune Response.” AQUACULTURE 520. doi:10.1016/j.aquaculture.2020.735012.
Vancouver
1.
Iryani MTM, MacRae TH, Sorgeloos P, Muhammad TST, Danish-Daniel M, Tan MP, et al. RNA interference of Hsp70 in Artemia franciscana nauplii and its effect on morphology, growth, survival and immune response. AQUACULTURE. 2020;520.
IEEE
[1]
M. T. M. Iryani et al., “RNA interference of Hsp70 in Artemia franciscana nauplii and its effect on morphology, growth, survival and immune response,” AQUACULTURE, vol. 520, 2020.
@article{8650657,
  abstract     = {{Brine shrimp Artemia franciscana release either encysted embryos via oviparous reproduction or motile nauplii via ovoviviparous reproduction. Artemia cysts contain an abundant amount of ArHsp21, ArHsp22, artemin, and p26, small heat shock proteins which regulate diapause and stress tolerance of this branchiopod crustacean. However, not much is known of the role of molecular chaperone, Hsp70 in this animal. In this study, the immunostimulatory role of Hsp70 in A. franciscana was investigated, work which included examining the function of this protein in embryo development, growth, survival and stress tolerance. Knockdown of Hsp70 was successfully performed by RNA interference (RNAi), with specific Hsp70 dsRNA construct delivered to female broods by microinjection to knockdown the protein in nauplii. The loss of Hsp70 neither affected the growth nor morphology as Artemia nauplii hatched normally and thereafter grew to adult within 28 days, observations similar to the control animals, the latter injected with GFP dsRNA. The survival of nauplii lacking Hsp70 appeared lower upon 28 days culture but the differences were insignificant when comparisons were made with the controls (P > .05). On other aspect, knockdown of Hsp70 reduced approximately 31% survival upon Vibrio campbellii challenge, indicating that Hsp70 assisted in the tolerance of Artemia nauplii during pathogen infection, perhaps through immune stimulation. Initial findings revealed that Hsp70 knockdown did not affect the expression of prophenoloxidase (proPO), a crucial immune protein of the shrimp innate immunity. By examining the morphology, growth, viability and molecular changes upon Hsp70 elimination, this study contributes to our understanding of the important roles of Hsp70 in A. franciscana, an important live food species used in aquaculture.}},
  articleno    = {{735012}},
  author       = {{Iryani, Mat Taib Mimi and MacRae, Thomas H and Sorgeloos, Patrick and Muhammad, Tengku Sifzizul Tengku and Danish-Daniel, Muhd and Tan, Min Pau and Satyantini, Woro Hastuti and Wahid, Mohd Effendy Abd and Sun, Jingfeng and Lv, Aijun and Sung, Yeong Yik}},
  issn         = {{0044-8486}},
  journal      = {{AQUACULTURE}},
  keywords     = {{Hsp70,Artemia franciscana,Nauplii,RNA interference (RNAi),HEAT-SHOCK PROTEINS,PROPHENOLOXIDASE SYSTEM,TOLERANCE,LARVAE,GENES,DIFFERENTIATION,RESISTANCE,KNOCKDOWN,DIAPAUSE,PROTECTS}},
  language     = {{eng}},
  pages        = {{7}},
  title        = {{RNA interference of Hsp70 in Artemia franciscana nauplii and its effect on morphology, growth, survival and immune response}},
  url          = {{http://dx.doi.org/10.1016/j.aquaculture.2020.735012}},
  volume       = {{520}},
  year         = {{2020}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: