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No evidence for a role for antibodies during vaccination-induced enhancement of porcine reproductive and respiratory syndrome

(2019) VIRUSES-BASEL. 11(9).
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Abstract
Vaccination is one of the most important tools to protect pigs against infection with porcine reproductive and respiratory syndrome virus 1 (PRRSV-1). Although neutralizing antibodies are considered to represent an important mechanism of protective immunity, anti-PRRSV antibodies, in particular at subneutralizing concentrations, have also been reported to exacerbate PRRSV infection, probably through Fc gamma R-mediated uptake of antibody-opsonized PRRSV, resulting in enhanced infection of, and replication in, target cells. Therefore, we investigated this pathway using sera from an animal experiment in which vaccine-mediated enhancement of clinical symptoms was observed. Three groups of six pigs were vaccinated with an inactivated PRRSV vaccine based on the PRRSV-1 subtype 3 strain Lena and challenged after a single or a prime-boost immunization protocol, or injected with PBS. We specifically tested if sera obtained from these animals can enhance macrophage infections, viral shedding, or cytokine release at different dilutions. Neither the presence of neutralizing antibodies nor general anti-PRRSV antibodies, mediated an enhanced infection, increased viral release or cytokine production by macrophages. Taken together, our data indicate that the exacerbated disease was not caused by antibodies.
Keywords
VIRUS PRRSV INFECTION, MOUTH-DISEASE VIRUS, DEPENDENT ENHANCEMENT, NEUTRALIZING ANTIBODIES, HUMORAL IMMUNITY, PASSIVE TRANSFER, FC, REPLICATION, MACROPHAGES, PROTECTION, PRRSV-1, inactivated vaccine, homologous challenge, in vivo, antibody-dependent enhancement (ADE) of disease, monocyte-derived, macrophages

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Citation

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MLA
Sautter, Carmen A., et al. “No Evidence for a Role for Antibodies during Vaccination-Induced Enhancement of Porcine Reproductive and Respiratory Syndrome.” VIRUSES-BASEL, vol. 11, no. 9, 2019.
APA
Sautter, C. A., Trus, I., Nauwynck, H., & Summerfield, A. (2019). No evidence for a role for antibodies during vaccination-induced enhancement of porcine reproductive and respiratory syndrome. VIRUSES-BASEL, 11(9).
Chicago author-date
Sautter, Carmen A, Ivan Trus, Hans Nauwynck, and Artur Summerfield. 2019. “No Evidence for a Role for Antibodies during Vaccination-Induced Enhancement of Porcine Reproductive and Respiratory Syndrome.” VIRUSES-BASEL 11 (9).
Chicago author-date (all authors)
Sautter, Carmen A, Ivan Trus, Hans Nauwynck, and Artur Summerfield. 2019. “No Evidence for a Role for Antibodies during Vaccination-Induced Enhancement of Porcine Reproductive and Respiratory Syndrome.” VIRUSES-BASEL 11 (9).
Vancouver
1.
Sautter CA, Trus I, Nauwynck H, Summerfield A. No evidence for a role for antibodies during vaccination-induced enhancement of porcine reproductive and respiratory syndrome. VIRUSES-BASEL. 2019;11(9).
IEEE
[1]
C. A. Sautter, I. Trus, H. Nauwynck, and A. Summerfield, “No evidence for a role for antibodies during vaccination-induced enhancement of porcine reproductive and respiratory syndrome,” VIRUSES-BASEL, vol. 11, no. 9, 2019.
@article{8649965,
  abstract     = {Vaccination is one of the most important tools to protect pigs against infection with porcine reproductive and respiratory syndrome virus 1 (PRRSV-1). Although neutralizing antibodies are considered to represent an important mechanism of protective immunity, anti-PRRSV antibodies, in particular at subneutralizing concentrations, have also been reported to exacerbate PRRSV infection, probably through Fc gamma R-mediated uptake of antibody-opsonized PRRSV, resulting in enhanced infection of, and replication in, target cells. Therefore, we investigated this pathway using sera from an animal experiment in which vaccine-mediated enhancement of clinical symptoms was observed. Three groups of six pigs were vaccinated with an inactivated PRRSV vaccine based on the PRRSV-1 subtype 3 strain Lena and challenged after a single or a prime-boost immunization protocol, or injected with PBS. We specifically tested if sera obtained from these animals can enhance macrophage infections, viral shedding, or cytokine release at different dilutions. Neither the presence of neutralizing antibodies nor general anti-PRRSV antibodies, mediated an enhanced infection, increased viral release or cytokine production by macrophages. Taken together, our data indicate that the exacerbated disease was not caused by antibodies.},
  articleno    = {829},
  author       = {Sautter, Carmen A and Trus, Ivan and Nauwynck, Hans and Summerfield, Artur},
  issn         = {1999-4915},
  journal      = {VIRUSES-BASEL},
  keywords     = {VIRUS PRRSV INFECTION,MOUTH-DISEASE VIRUS,DEPENDENT ENHANCEMENT,NEUTRALIZING ANTIBODIES,HUMORAL IMMUNITY,PASSIVE TRANSFER,FC,REPLICATION,MACROPHAGES,PROTECTION,PRRSV-1,inactivated vaccine,homologous challenge,in vivo,antibody-dependent enhancement (ADE) of disease,monocyte-derived,macrophages},
  language     = {eng},
  number       = {9},
  pages        = {16},
  title        = {No evidence for a role for antibodies during vaccination-induced enhancement of porcine reproductive and respiratory syndrome},
  url          = {http://dx.doi.org/10.3390/v11090829},
  volume       = {11},
  year         = {2019},
}

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