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Cell line-dependent increase in cellular quercetin accumulation upon stress induced by valinomycin and lipopolysaccharide, but not by TNF-alpha

Hanne Vissenaekens (UGent) , Charlotte Grootaert (UGent) , Andreja Rajkovic (UGent) , Kristof De Schutter (UGent) , Katleen Raes (UGent) , Guy Smagghe (UGent) , Tom Van de Wiele (UGent) and John Van Camp (UGent)
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Abstract
As the interface between the luminal and internal environment, the intestinal epithelium is strongly exposed to food-related, host-related and microbial stress. Furthermore, the endothelial stress response plays an important role in vascular disease development, which may be improved upon consumption of dietary bioactives such as polyphenols. The impact of the latter, however, is largely individual-dependent and effects are, in most cases, only observed under mild diseased conditions. Here, it is hypothesized that the individual's stressor levels may contribute to this variable response. To this end, the impact of the stressors (i) valinomycin (as model for cereulide, food-related microbial metabolite), (ii) TNF-alpha (host-related) and (iii) lipopolysaccharide (gram-negative bacterial cell related) on flavonoid accumulation was investigated in several intestinal and endothelial cell lines. Flow cytometry, confocal microscopy and an in-house developed, robust and high-throughput spectro-fluorometric method, showed that quercetin accumulated in all tested cell lines in a dose-dependent manner. Upon stress induced by valinomycin and to a lesser extent by lipopolysaccharide, but not by TNF-alpha, an increased quercetin accumulation was observed in proliferating intestinal and endothelial cells and not In differentiated intestinal or quiescent endothelial cells. Therefore, flavonoid accumulation may be a potential cellular stress response mechanism which strongly depends on the applied stressor, flavonoid, cell line and even growth conditions. This opens perspectives for further understanding the mechanisms by which cellular stress may shape the individual's response to bioactive compounds.
Keywords
INTESTINAL EPITHELIAL-CELLS, NECROSIS-FACTOR-ALPHA, TOLL-LIKE RECEPTOR-4, P-GLYCOPROTEIN, FLAVONOID QUERCETIN, BACILLUS-CEREUS, CACO-2, METABOLISM, FOOD, BIOAVAILABILITY, Cell lines, Quercetin, Valinomycin, Tumor necrosis factor-alpha, Lipopolysaccharide, Diphenylboric acid 2-aminoethyl ester

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MLA
Vissenaekens, Hanne, et al. “Cell Line-Dependent Increase in Cellular Quercetin Accumulation upon Stress Induced by Valinomycin and Lipopolysaccharide, but Not by TNF-Alpha.” FOOD RESEARCH INTERNATIONAL, vol. 125, 2019.
APA
Vissenaekens, H., Grootaert, C., Rajkovic, A., De Schutter, K., Raes, K., Smagghe, G., … Van Camp, J. (2019). Cell line-dependent increase in cellular quercetin accumulation upon stress induced by valinomycin and lipopolysaccharide, but not by TNF-alpha. FOOD RESEARCH INTERNATIONAL, 125.
Chicago author-date
Vissenaekens, Hanne, Charlotte Grootaert, Andreja Rajkovic, Kristof De Schutter, Katleen Raes, Guy Smagghe, Tom Van de Wiele, and John Van Camp. 2019. “Cell Line-Dependent Increase in Cellular Quercetin Accumulation upon Stress Induced by Valinomycin and Lipopolysaccharide, but Not by TNF-Alpha.” FOOD RESEARCH INTERNATIONAL 125.
Chicago author-date (all authors)
Vissenaekens, Hanne, Charlotte Grootaert, Andreja Rajkovic, Kristof De Schutter, Katleen Raes, Guy Smagghe, Tom Van de Wiele, and John Van Camp. 2019. “Cell Line-Dependent Increase in Cellular Quercetin Accumulation upon Stress Induced by Valinomycin and Lipopolysaccharide, but Not by TNF-Alpha.” FOOD RESEARCH INTERNATIONAL 125.
Vancouver
1.
Vissenaekens H, Grootaert C, Rajkovic A, De Schutter K, Raes K, Smagghe G, et al. Cell line-dependent increase in cellular quercetin accumulation upon stress induced by valinomycin and lipopolysaccharide, but not by TNF-alpha. FOOD RESEARCH INTERNATIONAL. 2019;125.
IEEE
[1]
H. Vissenaekens et al., “Cell line-dependent increase in cellular quercetin accumulation upon stress induced by valinomycin and lipopolysaccharide, but not by TNF-alpha,” FOOD RESEARCH INTERNATIONAL, vol. 125, 2019.
@article{8648661,
  abstract     = {As the interface between the luminal and internal environment, the intestinal epithelium is strongly exposed to food-related, host-related and microbial stress. Furthermore, the endothelial stress response plays an important role in vascular disease development, which may be improved upon consumption of dietary bioactives such as polyphenols. The impact of the latter, however, is largely individual-dependent and effects are, in most cases, only observed under mild diseased conditions. Here, it is hypothesized that the individual's stressor levels may contribute to this variable response. To this end, the impact of the stressors (i) valinomycin (as model for cereulide, food-related microbial metabolite), (ii) TNF-alpha (host-related) and (iii) lipopolysaccharide (gram-negative bacterial cell related) on flavonoid accumulation was investigated in several intestinal and endothelial cell lines. Flow cytometry, confocal microscopy and an in-house developed, robust and high-throughput spectro-fluorometric method, showed that quercetin accumulated in all tested cell lines in a dose-dependent manner. Upon stress induced by valinomycin and to a lesser extent by lipopolysaccharide, but not by TNF-alpha, an increased quercetin accumulation was observed in proliferating intestinal and endothelial cells and not In differentiated intestinal or quiescent endothelial cells. Therefore, flavonoid accumulation may be a potential cellular stress response mechanism which strongly depends on the applied stressor, flavonoid, cell line and even growth conditions. This opens perspectives for further understanding the mechanisms by which cellular stress may shape the individual's response to bioactive compounds.},
  articleno    = {108596},
  author       = {Vissenaekens, Hanne and Grootaert, Charlotte and Rajkovic, Andreja and De Schutter, Kristof and Raes, Katleen and Smagghe, Guy and Van de Wiele, Tom and Van Camp, John},
  issn         = {0963-9969},
  journal      = {FOOD RESEARCH INTERNATIONAL},
  keywords     = {INTESTINAL EPITHELIAL-CELLS,NECROSIS-FACTOR-ALPHA,TOLL-LIKE RECEPTOR-4,P-GLYCOPROTEIN,FLAVONOID QUERCETIN,BACILLUS-CEREUS,CACO-2,METABOLISM,FOOD,BIOAVAILABILITY,Cell lines,Quercetin,Valinomycin,Tumor necrosis factor-alpha,Lipopolysaccharide,Diphenylboric acid 2-aminoethyl ester},
  language     = {eng},
  pages        = {12},
  title        = {Cell line-dependent increase in cellular quercetin accumulation upon stress induced by valinomycin and lipopolysaccharide, but not by TNF-alpha},
  url          = {http://dx.doi.org/10.1016/j.foodres.2019.108596},
  volume       = {125},
  year         = {2019},
}

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