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Quorum sensing molecules as a novel microbial factor impacting muscle cells

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Abstract
Skeletal muscle makes up the largest part of human body mass and a good maintenance of this organ is essential for general health. In accordance, muscle wasting, a frequent phenomenon in many diseases, is associated with functional decline and a decrease in quality of life. Unfortunately, due to a lack of knowledge of the underlying pathophysiology, no targeted therapies exist today to encounter muscle wasting. Recent studies suggest a role for the gut microbiome in muscle wasting, without the mediators of this gut-muscle axis being identified. Here we evaluated the possible effects of 75 quorum sensing molecules (QSM), traditionally only seen as intra-bacterial communication molecules, on C2C12 muscle cells, studying viability, differentiation, inflammation, mitochondrial changes and protein degradation as biological outcomes. The responses were evaluated using different approaches: median absolute deviation, quartiles, strictly standardized mean difference and robust strictly standardized mean difference. This study resulted in 30 QSM, with effects observed on C2C12 cells. Known producers of the 27 peptide QSM belong to species of the genus Staphylococcus, Streptococcus, Enterococcus, Bacillus, Lactobacillus and Escherichia, while the 3 non-peptide QSM are produced by a broad range of Gram-positive and Gram-negative bacteria. Altogether, these proof-of-concept findings provide the first evidence that QSM produced by microbiota play a role in the gut-muscle axis, opening new perspectives for diagnostic and therapeutic targets in muscle wasting diseases.
Keywords
Quorum sensing molecules, Microbiome, C2C12, Muscle, Muscle wasting disease

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MLA
De Spiegeleer, Anton, et al. “Quorum Sensing Molecules as a Novel Microbial Factor Impacting Muscle Cells.” Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, vol. 1866, no. 3, 2020.
APA
De Spiegeleer, A., Elewaut, D., Van Den Noortgate, N., Janssens, Y., Debunne, N., Van Langenhove, S., … Wynendaele, E. (2020). Quorum sensing molecules as a novel microbial factor impacting muscle cells. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1866(3).
Chicago author-date
De Spiegeleer, Anton, Dirk Elewaut, Nele Van Den Noortgate, Yorick Janssens, Nathan Debunne, Selien Van Langenhove, Srinath Govindarajan, Bart De Spiegeleer, and Evelien Wynendaele. 2020. “Quorum Sensing Molecules as a Novel Microbial Factor Impacting Muscle Cells.” Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1866 (3).
Chicago author-date (all authors)
De Spiegeleer, Anton, Dirk Elewaut, Nele Van Den Noortgate, Yorick Janssens, Nathan Debunne, Selien Van Langenhove, Srinath Govindarajan, Bart De Spiegeleer, and Evelien Wynendaele. 2020. “Quorum Sensing Molecules as a Novel Microbial Factor Impacting Muscle Cells.” Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1866 (3).
Vancouver
1.
De Spiegeleer A, Elewaut D, Van Den Noortgate N, Janssens Y, Debunne N, Van Langenhove S, et al. Quorum sensing molecules as a novel microbial factor impacting muscle cells. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2020;1866(3).
IEEE
[1]
A. De Spiegeleer et al., “Quorum sensing molecules as a novel microbial factor impacting muscle cells,” Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, vol. 1866, no. 3, 2020.
@article{8647538,
  abstract     = {Skeletal muscle makes up the largest part of human body mass and a good maintenance of this organ is essential for general health. In accordance, muscle wasting, a frequent phenomenon in many diseases, is associated with functional decline and a decrease in quality of life. Unfortunately, due to a lack of knowledge of the underlying pathophysiology, no targeted therapies exist today to encounter muscle wasting. Recent studies suggest a role for the gut microbiome in muscle wasting, without the mediators of this gut-muscle axis being identified.

Here we evaluated the possible effects of 75 quorum sensing molecules (QSM), traditionally only seen as intra-bacterial communication molecules, on C2C12 muscle cells, studying viability, differentiation, inflammation, mitochondrial changes and protein degradation as biological outcomes. The responses were evaluated using different approaches: median absolute deviation, quartiles, strictly standardized mean difference and robust strictly standardized mean difference.

This study resulted in 30 QSM, with effects observed on C2C12 cells. Known producers of the 27 peptide QSM belong to species of the genus Staphylococcus, Streptococcus, Enterococcus, Bacillus, Lactobacillus and Escherichia, while the 3 non-peptide QSM are produced by a broad range of Gram-positive and Gram-negative bacteria. Altogether, these proof-of-concept findings provide the first evidence that QSM produced by microbiota play a role in the gut-muscle axis, opening new perspectives for diagnostic and therapeutic targets in muscle wasting diseases.},
  articleno    = {165646},
  author       = {De Spiegeleer, Anton and Elewaut, Dirk and Van Den Noortgate, Nele and Janssens, Yorick and Debunne, Nathan and Van Langenhove, Selien and Govindarajan, Srinath and De Spiegeleer, Bart and Wynendaele, Evelien},
  issn         = {0925-4439},
  journal      = {Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease},
  keywords     = {Quorum sensing molecules,Microbiome,C2C12,Muscle,Muscle wasting disease},
  language     = {eng},
  number       = {3},
  title        = {Quorum sensing molecules as a novel microbial factor impacting muscle cells},
  url          = {http://dx.doi.org/10.1016/j.bbadis.2019.165646},
  volume       = {1866},
  year         = {2020},
}

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