Advanced search
1 file | 486.66 KB Add to list

In vitro rumen simulations show a reduced disappearance of deoxynivalenol, nivalenol and enniatin B at conditions of rumen acidosis and lower microbial activity

Sandra Debevere (UGent) , An Cools (UGent) , Siegrid De Baere (UGent) , Geert Haesaert (UGent) , Michael Rychlik, Siska Croubels (UGent) and Veerle Fievez (UGent)
(2020) TOXINS. 12(2).
Author
Organization
Abstract
Ruminants are generally considered to be less susceptible to the effects of mycotoxins than monogastric animals as the rumen microbiota are capable of detoxifying some of these toxins. Despite this potential degradation, mycotoxin-associated subclinical health problems are seen in dairy cows. In this research, the disappearance of several mycotoxins was determined in an in vitro rumen model and the effect of realistic concentrations of those mycotoxins on fermentation was assessed by volatile fatty acid production. In addition, two hypotheses were tested: (1) a lower rumen pH leads to a decreased degradation of mycotoxins and (2) rumen fluid of lactating cows degrade mycotoxins better than rumen fluid of non-lactating cows. Maize silage was spiked with a mixture of deoxynivalenol (DON), nivalenol (NIV), enniatin B (ENN B), mycophenolic acid (MPA), roquefortine C (ROQ-C) and zearalenone (ZEN). Fresh rumen fluid of two lactating cows (L) and two non-lactating cows (N) was added to a buffer of normal pH (6.8) and low pH (5.8), leading to four combinations (L6.8, L5.8, N6.8, N5.8), which were added to the spiked maize substrate. In this study, mycotoxins had no effect on volatile fatty acid production. However, not all mycotoxins fully disappeared during incubation. ENN B and ROQ-C disappeared only partially, whereas MPA showed almost no disappearance. The disappearance of DON, NIV, and ENN B was hampered when pH was low, especially when the inoculum of non-lactating cows was used. For ZEN, a limited transformation of ZEN to alpha-ZEL and beta-ZEL was observed, but only at pH 6.8. In conclusion, based on the type of mycotoxin and the ruminal conditions, mycotoxins can stay intact in the rumen.
Keywords
mycotoxins, maize silage, SARA, lactation stage, rumen fluid, UPLC-MS, MS, TOXIN-CONTAMINATED WHEAT, FEED-INTAKE LEVEL, MYCOPHENOLIC-ACID, CARRY-OVER, ROQUEFORTINE-C, GAS-PRODUCTION, AFLATOXIN B-1, DAIRY-CATTLE, ZEARALENONE, MYCOTOXINS

Downloads

  • 8646910.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 486.66 KB

Citation

Please use this url to cite or link to this publication:

MLA
Debevere, Sandra, et al. “In Vitro Rumen Simulations Show a Reduced Disappearance of Deoxynivalenol, Nivalenol and Enniatin B at Conditions of Rumen Acidosis and Lower Microbial Activity.” TOXINS, vol. 12, no. 2, 2020, doi:10.3390/toxins12020101.
APA
Debevere, S., Cools, A., De Baere, S., Haesaert, G., Rychlik, M., Croubels, S., & Fievez, V. (2020). In vitro rumen simulations show a reduced disappearance of deoxynivalenol, nivalenol and enniatin B at conditions of rumen acidosis and lower microbial activity. TOXINS, 12(2). https://doi.org/10.3390/toxins12020101
Chicago author-date
Debevere, Sandra, An Cools, Siegrid De Baere, Geert Haesaert, Michael Rychlik, Siska Croubels, and Veerle Fievez. 2020. “In Vitro Rumen Simulations Show a Reduced Disappearance of Deoxynivalenol, Nivalenol and Enniatin B at Conditions of Rumen Acidosis and Lower Microbial Activity.” TOXINS 12 (2). https://doi.org/10.3390/toxins12020101.
Chicago author-date (all authors)
Debevere, Sandra, An Cools, Siegrid De Baere, Geert Haesaert, Michael Rychlik, Siska Croubels, and Veerle Fievez. 2020. “In Vitro Rumen Simulations Show a Reduced Disappearance of Deoxynivalenol, Nivalenol and Enniatin B at Conditions of Rumen Acidosis and Lower Microbial Activity.” TOXINS 12 (2). doi:10.3390/toxins12020101.
Vancouver
1.
Debevere S, Cools A, De Baere S, Haesaert G, Rychlik M, Croubels S, et al. In vitro rumen simulations show a reduced disappearance of deoxynivalenol, nivalenol and enniatin B at conditions of rumen acidosis and lower microbial activity. TOXINS. 2020;12(2).
IEEE
[1]
S. Debevere et al., “In vitro rumen simulations show a reduced disappearance of deoxynivalenol, nivalenol and enniatin B at conditions of rumen acidosis and lower microbial activity,” TOXINS, vol. 12, no. 2, 2020.
@article{8647526,
  abstract     = {Ruminants are generally considered to be less susceptible to the effects of mycotoxins than monogastric animals as the rumen microbiota are capable of detoxifying some of these toxins. Despite this potential degradation, mycotoxin-associated subclinical health problems are seen in dairy cows. In this research, the disappearance of several mycotoxins was determined in an in vitro rumen model and the effect of realistic concentrations of those mycotoxins on fermentation was assessed by volatile fatty acid production. In addition, two hypotheses were tested: (1) a lower rumen pH leads to a decreased degradation of mycotoxins and (2) rumen fluid of lactating cows degrade mycotoxins better than rumen fluid of non-lactating cows. Maize silage was spiked with a mixture of deoxynivalenol (DON), nivalenol (NIV), enniatin B (ENN B), mycophenolic acid (MPA), roquefortine C (ROQ-C) and zearalenone (ZEN). Fresh rumen fluid of two lactating cows (L) and two non-lactating cows (N) was added to a buffer of normal pH (6.8) and low pH (5.8), leading to four combinations (L6.8, L5.8, N6.8, N5.8), which were added to the spiked maize substrate. In this study, mycotoxins had no effect on volatile fatty acid production. However, not all mycotoxins fully disappeared during incubation. ENN B and ROQ-C disappeared only partially, whereas MPA showed almost no disappearance. The disappearance of DON, NIV, and ENN B was hampered when pH was low, especially when the inoculum of non-lactating cows was used. For ZEN, a limited transformation of ZEN to alpha-ZEL and beta-ZEL was observed, but only at pH 6.8. In conclusion, based on the type of mycotoxin and the ruminal conditions, mycotoxins can stay intact in the rumen.},
  articleno    = {101},
  author       = {Debevere, Sandra and Cools, An and De Baere, Siegrid and Haesaert, Geert and Rychlik, Michael and Croubels, Siska and Fievez, Veerle},
  issn         = {2072-6651},
  journal      = {TOXINS},
  keywords     = {mycotoxins,maize silage,SARA,lactation stage,rumen fluid,UPLC-MS,MS,TOXIN-CONTAMINATED WHEAT,FEED-INTAKE LEVEL,MYCOPHENOLIC-ACID,CARRY-OVER,ROQUEFORTINE-C,GAS-PRODUCTION,AFLATOXIN B-1,DAIRY-CATTLE,ZEARALENONE,MYCOTOXINS},
  language     = {eng},
  number       = {2},
  pages        = {23},
  title        = {In vitro rumen simulations show a reduced disappearance of deoxynivalenol, nivalenol and enniatin B at conditions of rumen acidosis and lower microbial activity},
  url          = {http://dx.doi.org/10.3390/toxins12020101},
  volume       = {12},
  year         = {2020},
}

Altmetric
View in Altmetric
Web of Science
Times cited: