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A methodology for discovering novel brain-relevant peptides : combination of ribosome profiling and peptidomics

(2020) NEUROSCIENCE RESEARCH. 151. p.31-37
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Abstract
Brain derived peptides function as signaling molecules in the brain and regulate various physiological and behavioral processes. The low abundance and atypical fragmentation of these brain derived peptides makes detection using traditional proteomic methods challenging. In this study, we introduce and validate a new methodology for the discovery of novel peptides derived from mammalian brain. This methodology combines ribosome profiling and mass spectrometry-based peptidomics. Using this framework, we have identified a novel peptide in mouse whole brain whose expression is highest in the basal ganglia, hypothalamus and amygdala. Although its functional role is unknown, it has been previously detected in peripheral tissue as a component of the mRNA decapping complex. Continued discovery and studies of novel regulating peptides in mammalian brain may also provide insight into brain disorders. (C) 2019 Published by Elsevier B.V.
Keywords
General Neuroscience, General Medicine, Brain peptides, Ribosome profiling, Peptidomics, Mass spectrometry, NEUROPEPTIDES, TRANSLATION, RECEPTOR, REVEALS, ROLES, SLEEP, MUTATION, OREXINS, STEM

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MLA
Tharakan, Ravi, et al. “A Methodology for Discovering Novel Brain-Relevant Peptides : Combination of Ribosome Profiling and Peptidomics.” NEUROSCIENCE RESEARCH, vol. 151, 2020, pp. 31–37, doi:10.1016/j.neures.2019.02.006.
APA
Tharakan, R., Kreimer, S., Ubaida-Mohien, C., Lavoie, J., Olexiouk, V., Menschaert, G., … Nucifora, L. G. (2020). A methodology for discovering novel brain-relevant peptides : combination of ribosome profiling and peptidomics. NEUROSCIENCE RESEARCH, 151, 31–37. https://doi.org/10.1016/j.neures.2019.02.006
Chicago author-date
Tharakan, Ravi, Simion Kreimer, Ceereena Ubaida-Mohien, Joelle Lavoie, Volodimir Olexiouk, Gerben Menschaert, Nicholas T. Ingolia, et al. 2020. “A Methodology for Discovering Novel Brain-Relevant Peptides : Combination of Ribosome Profiling and Peptidomics.” NEUROSCIENCE RESEARCH 151: 31–37. https://doi.org/10.1016/j.neures.2019.02.006.
Chicago author-date (all authors)
Tharakan, Ravi, Simion Kreimer, Ceereena Ubaida-Mohien, Joelle Lavoie, Volodimir Olexiouk, Gerben Menschaert, Nicholas T. Ingolia, Robert N. Cole, Koko Ishizuka, Akira Sawa, and Leslie G. Nucifora. 2020. “A Methodology for Discovering Novel Brain-Relevant Peptides : Combination of Ribosome Profiling and Peptidomics.” NEUROSCIENCE RESEARCH 151: 31–37. doi:10.1016/j.neures.2019.02.006.
Vancouver
1.
Tharakan R, Kreimer S, Ubaida-Mohien C, Lavoie J, Olexiouk V, Menschaert G, et al. A methodology for discovering novel brain-relevant peptides : combination of ribosome profiling and peptidomics. NEUROSCIENCE RESEARCH. 2020;151:31–7.
IEEE
[1]
R. Tharakan et al., “A methodology for discovering novel brain-relevant peptides : combination of ribosome profiling and peptidomics,” NEUROSCIENCE RESEARCH, vol. 151, pp. 31–37, 2020.
@article{8647111,
  abstract     = {{Brain derived peptides function as signaling molecules in the brain and regulate various physiological and behavioral processes. The low abundance and atypical fragmentation of these brain derived peptides makes detection using traditional proteomic methods challenging. In this study, we introduce and validate a new methodology for the discovery of novel peptides derived from mammalian brain. This methodology combines ribosome profiling and mass spectrometry-based peptidomics. Using this framework, we have identified a novel peptide in mouse whole brain whose expression is highest in the basal ganglia, hypothalamus and amygdala. Although its functional role is unknown, it has been previously detected in peripheral tissue as a component of the mRNA decapping complex. Continued discovery and studies of novel regulating peptides in mammalian brain may also provide insight into brain disorders. (C) 2019 Published by Elsevier B.V.}},
  author       = {{Tharakan, Ravi and Kreimer, Simion and Ubaida-Mohien, Ceereena and Lavoie, Joelle and Olexiouk, Volodimir and Menschaert, Gerben and Ingolia, Nicholas T. and Cole, Robert N. and Ishizuka, Koko and Sawa, Akira and Nucifora, Leslie G.}},
  issn         = {{0168-0102}},
  journal      = {{NEUROSCIENCE RESEARCH}},
  keywords     = {{General Neuroscience,General Medicine,Brain peptides,Ribosome profiling,Peptidomics,Mass spectrometry,NEUROPEPTIDES,TRANSLATION,RECEPTOR,REVEALS,ROLES,SLEEP,MUTATION,OREXINS,STEM}},
  language     = {{eng}},
  pages        = {{31--37}},
  title        = {{A methodology for discovering novel brain-relevant peptides : combination of ribosome profiling and peptidomics}},
  url          = {{http://dx.doi.org/10.1016/j.neures.2019.02.006}},
  volume       = {{151}},
  year         = {{2020}},
}

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