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High-resolution mass spectrometry-based selection of peanut peptide biomarkers considering food processing and market type variation

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Abstract
To protect allergic patients and guarantee correct food labeling, robust, specific and sensitive detection methods are urgently needed. Mass spectrometry (MS)-based methods could overcome the limitations of current detection techniques. The first step in the development of an MS-based method is the identification of biomarkers, which are, in the case of food allergens, peptides. Here, we implemented a strategy to identify the most salient peptide biomarkers in peanuts. Processed peanut matrices were prepared and analyzed using an untargeted approach via high-resolution MS. More than 300 identified peptides were further filtered using selection criteria to strengthen the analytical performance of a future, routine quantitative method. The resulting 16 peptides are robust to food processing, specific to peanuts, and satisfy sequence-based criteria. The aspect of multiple protein isoforms is also considered in the selection tree, an aspect that is essential for a quantitative method's robustness but seldom, if ever, considered.
Keywords
High-resolution mass spectrometry, Peptide biomarker selection, Processed food products, Multiple allergen isoforms, Peanut origin, MAJOR ALLERGEN, DEAMIDATION, ASPARAGINYL, GLUTAMINYL, PREDICTION, BEHAVIOR, PROTEIN

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MLA
Gavage, Maxime, et al. “High-Resolution Mass Spectrometry-Based Selection of Peanut Peptide Biomarkers Considering Food Processing and Market Type Variation.” FOOD CHEMISTRY, vol. 304, 2020.
APA
Gavage, M., Van Vlierberghe, K., Van Poucke, C., De Loose, M., Gevaert, K., Dieu, M., … Gillard, N. (2020). High-resolution mass spectrometry-based selection of peanut peptide biomarkers considering food processing and market type variation. FOOD CHEMISTRY, 304.
Chicago author-date
Gavage, Maxime, Kaatje Van Vlierberghe, Christof Van Poucke, Marc De Loose, Kris Gevaert, Marc Dieu, Patsy Renard, Thierry Arnould, and Nathalie Gillard. 2020. “High-Resolution Mass Spectrometry-Based Selection of Peanut Peptide Biomarkers Considering Food Processing and Market Type Variation.” FOOD CHEMISTRY 304.
Chicago author-date (all authors)
Gavage, Maxime, Kaatje Van Vlierberghe, Christof Van Poucke, Marc De Loose, Kris Gevaert, Marc Dieu, Patsy Renard, Thierry Arnould, and Nathalie Gillard. 2020. “High-Resolution Mass Spectrometry-Based Selection of Peanut Peptide Biomarkers Considering Food Processing and Market Type Variation.” FOOD CHEMISTRY 304.
Vancouver
1.
Gavage M, Van Vlierberghe K, Van Poucke C, De Loose M, Gevaert K, Dieu M, et al. High-resolution mass spectrometry-based selection of peanut peptide biomarkers considering food processing and market type variation. FOOD CHEMISTRY. 2020;304.
IEEE
[1]
M. Gavage et al., “High-resolution mass spectrometry-based selection of peanut peptide biomarkers considering food processing and market type variation,” FOOD CHEMISTRY, vol. 304, 2020.
@article{8646988,
  abstract     = {To protect allergic patients and guarantee correct food labeling, robust, specific and sensitive detection methods are urgently needed. Mass spectrometry (MS)-based methods could overcome the limitations of current detection techniques. The first step in the development of an MS-based method is the identification of biomarkers, which are, in the case of food allergens, peptides. Here, we implemented a strategy to identify the most salient peptide biomarkers in peanuts. Processed peanut matrices were prepared and analyzed using an untargeted approach via high-resolution MS. More than 300 identified peptides were further filtered using selection criteria to strengthen the analytical performance of a future, routine quantitative method. The resulting 16 peptides are robust to food processing, specific to peanuts, and satisfy sequence-based criteria. The aspect of multiple protein isoforms is also considered in the selection tree, an aspect that is essential for a quantitative method's robustness but seldom, if ever, considered.},
  articleno    = {125428},
  author       = {Gavage, Maxime and Van Vlierberghe, Kaatje and Van Poucke, Christof and De Loose, Marc and Gevaert, Kris and Dieu, Marc and Renard, Patsy and Arnould, Thierry and Gillard, Nathalie},
  issn         = {0308-8146},
  journal      = {FOOD CHEMISTRY},
  keywords     = {High-resolution mass spectrometry,Peptide biomarker selection,Processed food products,Multiple allergen isoforms,Peanut origin,MAJOR ALLERGEN,DEAMIDATION,ASPARAGINYL,GLUTAMINYL,PREDICTION,BEHAVIOR,PROTEIN},
  language     = {eng},
  pages        = {10},
  title        = {High-resolution mass spectrometry-based selection of peanut peptide biomarkers considering food processing and market type variation},
  url          = {http://dx.doi.org/10.1016/j.foodchem.2019.125428},
  volume       = {304},
  year         = {2020},
}

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