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A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN‐FORMED protein polarity and relocalisation in Arabidopsis

Siamsa M. Doyle, Adeline Rigal, Peter Grones, Michal Karady, Deepak K. Barange, Mateusz Majda, Barbora Pařízková, Michail Karampelias (UGent) , Marta Zwiewka, Aleš Pěnčík, et al.
(2019) NEW PHYTOLOGIST. 223(3). p.1420-1432
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Abstract
Distribution of auxin within plant tissues is of great importance for developmental plasticity, including root gravitropic growth. Auxin flow is directed by the subcellular polar distribution and dynamic relocalisation of auxin transporters such as the PIN-FORMED (PIN) efflux carriers, which can be influenced by the main natural plant auxin indole-3-acetic acid (IAA). Anthranilic acid (AA) is an important early precursor of IAA and previously published studies with AA analogues have suggested that AA may also regulate PIN localisation. Using Arabidopsis thaliana as a model species, we studied an AA-deficient mutant displaying agravitropic root growth, treated seedlings with AA and AA analogues and transformed lines to over-produce AA while inhibiting its conversion to downstream IAA precursors. We showed that AA rescues root gravitropic growth in the AA-deficient mutant at concentrations that do not rescue IAA levels. Overproduction of AA affects root gravitropism without affecting IAA levels. Treatments with, or deficiency in, AA result in defects in PIN polarity and gravistimulus-induced PIN relocalisation in root cells. Our results revealed a previously unknown role for AA in the regulation of PIN subcellular localisation and dynamics involved in root gravitropism, which is independent of its better known role in IAA biosynthesis.
Keywords
Arabidopsis thaliana, PIN polarity, PIN-FORMED proteins, anthranilic acid (AA), auxin transport, root gravitropism, TRANSPORT, PATHWAY, EFFLUX, BIOSYNTHESIS, TRAFFICKING, ENDOCYTOSIS, CARRIER, GENES

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MLA
Doyle, Siamsa M., et al. “A Role for the Auxin Precursor Anthranilic Acid in Root Gravitropism via Regulation of PIN‐FORMED Protein Polarity and Relocalisation in Arabidopsis.” NEW PHYTOLOGIST, vol. 223, no. 3, 2019, pp. 1420–32, doi:10.1111/nph.15877.
APA
Doyle, S. M., Rigal, A., Grones, P., Karady, M., Barange, D. K., Majda, M., … Robert, S. (2019). A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN‐FORMED protein polarity and relocalisation in Arabidopsis. NEW PHYTOLOGIST, 223(3), 1420–1432. https://doi.org/10.1111/nph.15877
Chicago author-date
Doyle, Siamsa M., Adeline Rigal, Peter Grones, Michal Karady, Deepak K. Barange, Mateusz Majda, Barbora Pařízková, et al. 2019. “A Role for the Auxin Precursor Anthranilic Acid in Root Gravitropism via Regulation of PIN‐FORMED Protein Polarity and Relocalisation in Arabidopsis.” NEW PHYTOLOGIST 223 (3): 1420–32. https://doi.org/10.1111/nph.15877.
Chicago author-date (all authors)
Doyle, Siamsa M., Adeline Rigal, Peter Grones, Michal Karady, Deepak K. Barange, Mateusz Majda, Barbora Pařízková, Michail Karampelias, Marta Zwiewka, Aleš Pěnčík, Fredrik Almqvist, Karin Ljung, Ondřej Novák, and Stéphanie Robert. 2019. “A Role for the Auxin Precursor Anthranilic Acid in Root Gravitropism via Regulation of PIN‐FORMED Protein Polarity and Relocalisation in Arabidopsis.” NEW PHYTOLOGIST 223 (3): 1420–1432. doi:10.1111/nph.15877.
Vancouver
1.
Doyle SM, Rigal A, Grones P, Karady M, Barange DK, Majda M, et al. A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN‐FORMED protein polarity and relocalisation in Arabidopsis. NEW PHYTOLOGIST. 2019;223(3):1420–32.
IEEE
[1]
S. M. Doyle et al., “A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN‐FORMED protein polarity and relocalisation in Arabidopsis,” NEW PHYTOLOGIST, vol. 223, no. 3, pp. 1420–1432, 2019.
@article{8646762,
  abstract     = {{Distribution of auxin within plant tissues is of great importance for developmental plasticity, including root gravitropic growth. Auxin flow is directed by the subcellular polar distribution and dynamic relocalisation of auxin transporters such as the PIN-FORMED (PIN) efflux carriers, which can be influenced by the main natural plant auxin indole-3-acetic acid (IAA). Anthranilic acid (AA) is an important early precursor of IAA and previously published studies with AA analogues have suggested that AA may also regulate PIN localisation. Using Arabidopsis thaliana as a model species, we studied an AA-deficient mutant displaying agravitropic root growth, treated seedlings with AA and AA analogues and transformed lines to over-produce AA while inhibiting its conversion to downstream IAA precursors. We showed that AA rescues root gravitropic growth in the AA-deficient mutant at concentrations that do not rescue IAA levels. Overproduction of AA affects root gravitropism without affecting IAA levels. Treatments with, or deficiency in, AA result in defects in PIN polarity and gravistimulus-induced PIN relocalisation in root cells. Our results revealed a previously unknown role for AA in the regulation of PIN subcellular localisation and dynamics involved in root gravitropism, which is independent of its better known role in IAA biosynthesis.}},
  author       = {{Doyle, Siamsa M. and Rigal, Adeline and Grones, Peter and Karady, Michal and Barange, Deepak K. and Majda, Mateusz and Pařízková, Barbora and Karampelias, Michail and Zwiewka, Marta and Pěnčík, Aleš and Almqvist, Fredrik and Ljung, Karin and Novák, Ondřej and Robert, Stéphanie}},
  issn         = {{0028-646X}},
  journal      = {{NEW PHYTOLOGIST}},
  keywords     = {{Arabidopsis thaliana,PIN polarity,PIN-FORMED proteins,anthranilic acid (AA),auxin transport,root gravitropism,TRANSPORT,PATHWAY,EFFLUX,BIOSYNTHESIS,TRAFFICKING,ENDOCYTOSIS,CARRIER,GENES}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{1420--1432}},
  title        = {{A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN‐FORMED protein polarity and relocalisation in Arabidopsis}},
  url          = {{http://doi.org/10.1111/nph.15877}},
  volume       = {{223}},
  year         = {{2019}},
}
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