Methodology for the treatment of increment cores to assess the vessel size distribution of a tree along a pith-to-bark transect
- Author
- Félix Laurent, Victor Deklerck, Emmanuel Kasongo Yakusu (UGent) , Kévin Lievens, Tom De Mil (UGent) , Wannes Hubau (UGent) and Hans Beeckman
- Organization
- Abstract
- The sampling of increment cores constitutes a non-destructive approach to study the wood anatomy of a tree along a radial transect. The distribution and variability of anatomical features along such a transect can serve as a proxy of a tree’s growth dynamics. It is therefore valuable to develop methods of accessing and analyzing these data, such as the distribution of vessel sizes along a pith-to-bark transect. The processing of the increment cores begins with the production of long cross sections, 10 to 20 µm thick, using a microtome. The cross sections are then stained to highlight the anatomical features. Next, the cross sections are digitized using the Stream Motion software (Olympus Optical Co. Japan) on a scanning stage (SCAN 100 x 100, Märzhäuser Wetzlar, Germany) to create high resolution images. A pre-treatment of the images, with an image manipulation program such as GIMP 2 (www.gimp.org), is then required to enable their use for data analyses. This includes associating fragments to reconstruct a linear and continuous cross section, since it is difficult to consistently prevent the formation of tearing when cutting the cores. The data contained in the cross section images is finally accessed using the ImageJ software (www.imagej.net). Data such as the position, size, and vessels density along a pith-to-bark transect are then acquired and can therefore be used to study the physiology and growth dynamics of the trees. ImageJ features such as the Trainable Weka Segmentation plugin offer the opportunity to automatize the analyses, therefore allowing for a larger sampling size and faster processing.
- Keywords
- Treatement method, tree cores, vessel size, pith-to-bark transect
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8646641
- MLA
- Laurent, Félix, et al. “Methodology for the Treatment of Increment Cores to Assess the Vessel Size Distribution of a Tree along a Pith-to-Bark Transect.” AMPEE5, 5th Annual Meeting on Plant Ecology and Evolution, Abstracts, Botanical Society Belgium and Meise Botanic Garden, 2019.
- APA
- Laurent, F., Deklerck, V., Kasongo Yakusu, E., Lievens, K., De Mil, T., Hubau, W., & Beeckman, H. (2019). Methodology for the treatment of increment cores to assess the vessel size distribution of a tree along a pith-to-bark transect. AMPEE5, 5th Annual Meeting on Plant Ecology and Evolution, Abstracts. Presented at the Fitth Annual Meeting on Plant Ecology and Evolution, Meise, Belgium.
- Chicago author-date
- Laurent, Félix, Victor Deklerck, Emmanuel Kasongo Yakusu, Kévin Lievens, Tom De Mil, Wannes Hubau, and Hans Beeckman. 2019. “Methodology for the Treatment of Increment Cores to Assess the Vessel Size Distribution of a Tree along a Pith-to-Bark Transect.” In AMPEE5, 5th Annual Meeting on Plant Ecology and Evolution, Abstracts. Meise: Botanical Society Belgium and Meise Botanic Garden.
- Chicago author-date (all authors)
- Laurent, Félix, Victor Deklerck, Emmanuel Kasongo Yakusu, Kévin Lievens, Tom De Mil, Wannes Hubau, and Hans Beeckman. 2019. “Methodology for the Treatment of Increment Cores to Assess the Vessel Size Distribution of a Tree along a Pith-to-Bark Transect.” In AMPEE5, 5th Annual Meeting on Plant Ecology and Evolution, Abstracts. Meise: Botanical Society Belgium and Meise Botanic Garden.
- Vancouver
- 1.Laurent F, Deklerck V, Kasongo Yakusu E, Lievens K, De Mil T, Hubau W, et al. Methodology for the treatment of increment cores to assess the vessel size distribution of a tree along a pith-to-bark transect. In: AMPEE5, 5th Annual Meeting on Plant Ecology and Evolution, Abstracts. Meise: Botanical Society Belgium and Meise Botanic Garden; 2019.
- IEEE
- [1]F. Laurent et al., “Methodology for the treatment of increment cores to assess the vessel size distribution of a tree along a pith-to-bark transect,” in AMPEE5, 5th Annual Meeting on Plant Ecology and Evolution, Abstracts, Meise, Belgium, 2019.
@inproceedings{8646641,
abstract = {{The sampling of increment cores constitutes a non-destructive approach to study the wood anatomy of a tree along a radial transect. The distribution and variability of anatomical features along such a transect can serve as a proxy of a tree’s growth dynamics. It is therefore valuable to develop methods of accessing and analyzing these data, such as the distribution of vessel sizes along a pith-to-bark transect.
The processing of the increment cores begins with the production of long cross sections, 10 to 20 µm thick, using a microtome. The cross sections are then stained to highlight the anatomical features. Next, the cross sections are digitized using the Stream Motion software (Olympus Optical Co. Japan) on a scanning stage (SCAN 100 x 100, Märzhäuser Wetzlar, Germany) to create high resolution images. A pre-treatment of the images, with an image manipulation program such as GIMP 2 (www.gimp.org), is then required to enable their use for data analyses. This includes associating fragments to reconstruct a linear and continuous cross section, since it is difficult to consistently prevent the formation of tearing when cutting the cores.
The data contained in the cross section images is finally accessed using the ImageJ software (www.imagej.net). Data such as the position, size, and vessels density along a pith-to-bark transect are then acquired and can therefore be used to study the physiology and growth dynamics of the trees. ImageJ features such as the Trainable Weka Segmentation plugin offer the opportunity to automatize the analyses, therefore allowing for a larger sampling size and faster processing.}},
author = {{Laurent, Félix and Deklerck, Victor and Kasongo Yakusu, Emmanuel and Lievens, Kévin and De Mil, Tom and Hubau, Wannes and Beeckman, Hans}},
booktitle = {{AMPEE5, 5th Annual Meeting on Plant Ecology and Evolution, Abstracts}},
keywords = {{Treatement method,tree cores,vessel size,pith-to-bark transect}},
language = {{eng}},
location = {{Meise, Belgium}},
publisher = {{Botanical Society Belgium and Meise Botanic Garden}},
title = {{Methodology for the treatment of increment cores to assess the vessel size distribution of a tree along a pith-to-bark transect}},
url = {{https://sites.google.com/plantentuinmeise.be/ampee5/homepage/ampee5-abstracts}},
year = {{2019}},
}