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Metabolic profile, bioavailability and toxicokinetics of zearalenone-14-glucoside in rats after oral and intravenous administration by liquid chromatography high-resolution mass spectrometry and tandem mass spectrometry

Feifei Sun, Haiguang Tan, Yanshen Li, Marthe De Boevre (UGent) , Sarah De Saeger (UGent) , Jinhui Zhou, Yi Li, Zhenghua Rao, Shupeng Yang and Huiyan Zhang
(2019) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 20(21).
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Abstract
Zearalenone-14-glucoside (ZEN-14G), a key modified mycotoxin, has attracted a great deal of attention due to the possible conversion to its free form of zearalenone (ZEN) exerting toxicity. In this study, the toxicokinetics of ZEN-14G were investigated in rats after oral and intravenous administration. The plasma concentrations of ZEN-14G and its major five metabolites were quantified using a validated liquid chromatography tandem mass spectrometry (LC-MS/MS) method. The data were analyzed via non-compartmental analysis using software WinNonlin 6.3. The results indicated that ZEN-14G was rapidly hydrolyzed into ZEN in vivo. In addition, the major parameters of ZEN-14G following intravenous administration were: area under the plasma concentration-time curve (AUC), 1.80 h.ng/mL; the apparent volume of distribution (V-Z), 7.25 L/kg; and total body clearance (CL), 5.02 mL/h/kg, respectively. After oral administration, the typical parameters were: AUC, 0.16 h.ng/mL; V-Z, 6.24 mL/kg; and CL, 4.50 mL/h/kg, respectively. The absolute oral bioavailability of ZEN-14G in rats was about 9%, since low levels of ZEN-14G were detected in plasma, which might be attributed to its extensive metabolism. Therefore, liquid chromatography high-resolution mass spectrometry (LC-HRMS) was adopted to clarify the metabolic profile of ZEN-14G in rats' plasma. As a result, eight metabolites were identified in which ZEN-14-glucuronic acid (ZEN-14GlcA) had a large yield from the first time-point and continued accumulating after oral administration, indicating that ZEN-14-glucuronic acid could serve a potential biomarker of ZEN-14G. The obtained outcomes would prompt the accurate safety evaluation of ZEN-14G.
Keywords
MASKED MYCOTOXIN, ZEARALENONE, CEREALS, PLASMA, masked mycotoxins, zearalenone, metabolism, bioavailability, risk, assessment

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MLA
Sun, Feifei, et al. “Metabolic Profile, Bioavailability and Toxicokinetics of Zearalenone-14-Glucoside in Rats after Oral and Intravenous Administration by Liquid Chromatography High-Resolution Mass Spectrometry and Tandem Mass Spectrometry.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 21, 2019, doi:10.3390/ijms20215473.
APA
Sun, F., Tan, H., Li, Y., De Boevre, M., De Saeger, S., Zhou, J., … Zhang, H. (2019). Metabolic profile, bioavailability and toxicokinetics of zearalenone-14-glucoside in rats after oral and intravenous administration by liquid chromatography high-resolution mass spectrometry and tandem mass spectrometry. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(21). https://doi.org/10.3390/ijms20215473
Chicago author-date
Sun, Feifei, Haiguang Tan, Yanshen Li, Marthe De Boevre, Sarah De Saeger, Jinhui Zhou, Yi Li, Zhenghua Rao, Shupeng Yang, and Huiyan Zhang. 2019. “Metabolic Profile, Bioavailability and Toxicokinetics of Zearalenone-14-Glucoside in Rats after Oral and Intravenous Administration by Liquid Chromatography High-Resolution Mass Spectrometry and Tandem Mass Spectrometry.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20 (21). https://doi.org/10.3390/ijms20215473.
Chicago author-date (all authors)
Sun, Feifei, Haiguang Tan, Yanshen Li, Marthe De Boevre, Sarah De Saeger, Jinhui Zhou, Yi Li, Zhenghua Rao, Shupeng Yang, and Huiyan Zhang. 2019. “Metabolic Profile, Bioavailability and Toxicokinetics of Zearalenone-14-Glucoside in Rats after Oral and Intravenous Administration by Liquid Chromatography High-Resolution Mass Spectrometry and Tandem Mass Spectrometry.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20 (21). doi:10.3390/ijms20215473.
Vancouver
1.
Sun F, Tan H, Li Y, De Boevre M, De Saeger S, Zhou J, et al. Metabolic profile, bioavailability and toxicokinetics of zearalenone-14-glucoside in rats after oral and intravenous administration by liquid chromatography high-resolution mass spectrometry and tandem mass spectrometry. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2019;20(21).
IEEE
[1]
F. Sun et al., “Metabolic profile, bioavailability and toxicokinetics of zearalenone-14-glucoside in rats after oral and intravenous administration by liquid chromatography high-resolution mass spectrometry and tandem mass spectrometry,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 21, 2019.
@article{8646513,
  abstract     = {{Zearalenone-14-glucoside (ZEN-14G), a key modified mycotoxin, has attracted a great deal of attention due to the possible conversion to its free form of zearalenone (ZEN) exerting toxicity. In this study, the toxicokinetics of ZEN-14G were investigated in rats after oral and intravenous administration. The plasma concentrations of ZEN-14G and its major five metabolites were quantified using a validated liquid chromatography tandem mass spectrometry (LC-MS/MS) method. The data were analyzed via non-compartmental analysis using software WinNonlin 6.3. The results indicated that ZEN-14G was rapidly hydrolyzed into ZEN in vivo. In addition, the major parameters of ZEN-14G following intravenous administration were: area under the plasma concentration-time curve (AUC), 1.80 h.ng/mL; the apparent volume of distribution (V-Z), 7.25 L/kg; and total body clearance (CL), 5.02 mL/h/kg, respectively. After oral administration, the typical parameters were: AUC, 0.16 h.ng/mL; V-Z, 6.24 mL/kg; and CL, 4.50 mL/h/kg, respectively. The absolute oral bioavailability of ZEN-14G in rats was about 9%, since low levels of ZEN-14G were detected in plasma, which might be attributed to its extensive metabolism. Therefore, liquid chromatography high-resolution mass spectrometry (LC-HRMS) was adopted to clarify the metabolic profile of ZEN-14G in rats' plasma. As a result, eight metabolites were identified in which ZEN-14-glucuronic acid (ZEN-14GlcA) had a large yield from the first time-point and continued accumulating after oral administration, indicating that ZEN-14-glucuronic acid could serve a potential biomarker of ZEN-14G. The obtained outcomes would prompt the accurate safety evaluation of ZEN-14G.}},
  articleno    = {{5473}},
  author       = {{Sun, Feifei and Tan, Haiguang and Li, Yanshen and De Boevre, Marthe and De Saeger, Sarah and Zhou, Jinhui and Li, Yi and Rao, Zhenghua and Yang, Shupeng and Zhang, Huiyan}},
  issn         = {{1422-0067}},
  journal      = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
  keywords     = {{MASKED MYCOTOXIN,ZEARALENONE,CEREALS,PLASMA,masked mycotoxins,zearalenone,metabolism,bioavailability,risk,assessment}},
  language     = {{eng}},
  number       = {{21}},
  pages        = {{11}},
  title        = {{Metabolic profile, bioavailability and toxicokinetics of zearalenone-14-glucoside in rats after oral and intravenous administration by liquid chromatography high-resolution mass spectrometry and tandem mass spectrometry}},
  url          = {{http://doi.org/10.3390/ijms20215473}},
  volume       = {{20}},
  year         = {{2019}},
}
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