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Targeting miR‑155‑5p and miR‑221‑3p by peptide nucleic acids induces caspase‑3 activation and apoptosis in temozolomide‑resistant T98G glioma cells

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Abstract
The present study investigated the effects of the combined treatment of two peptide nucleic acids (PNAs), directed against microRNAs involved in caspase-3 mRNA regulation (miR-155-5p and miR-221-3p) in the temozolomide (TMZ)-resistant T98G glioma cell line. These PNAs were conjugated with an octaarginine tail in order to obtain an efficient delivery to treated cells. The effects of singularly administered PNAs or a combined treatment with both PNAs were examined on apoptosis, with the aim to determine whether reversion of the drug-resistance phenotype was obtained. Specificity of the PNA-mediated effects was analyzed by reverse transcription-quantitative polymerase-chain reaction, which demonstrated that the effects of R8-PNA-a155 and R8-PNA-a221 anti-miR PNAs were specific. Furthermore, the results obtained confirmed that both PNAs induced apoptosis when used on the temozolomide-resistant T98G glioma cell line. Notably, co-administration of both anti-miR-155 and anti-miR-221 PNAs was associated with an increased proapoptotic activity. In addition, TMZ further increased the induction of apoptosis in T98G cells co-treated with anti-miR-155 and anti-miR-221 PNAs.
Keywords
peptide nucleic acids, glioma, microRNAs, miR-221-3p, miR-155-5p, miRNA targeting, delivery, apoptosis, temozolomide, GENE-EXPRESSION, BIOLOGICAL-ACTIVITY, MICRORNA FUNCTIONS, STEM-CELLS, GLIOBLASTOMA, CANCER, PNA, INHIBITION, GROWTH, DNA

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MLA
Milani, Roberta, et al. “Targeting MiR‑155‑5p and MiR‑221‑3p by Peptide Nucleic Acids Induces Caspase‑3 Activation and Apoptosis in Temozolomide‑resistant T98G Glioma Cells.” INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 55, no. 1, 2019, pp. 59–68.
APA
Milani, R., Brognara, E., Fabbri, E., Manicardi, A., Corradini, R., Finotti, A., … Gambari, R. (2019). Targeting miR‑155‑5p and miR‑221‑3p by peptide nucleic acids induces caspase‑3 activation and apoptosis in temozolomide‑resistant T98G glioma cells. INTERNATIONAL JOURNAL OF ONCOLOGY, 55(1), 59–68.
Chicago author-date
Milani, Roberta, Eleonora Brognara, Enrica Fabbri, Alex Manicardi, Roberto Corradini, Alessia Finotti, Jessica Gasparello, et al. 2019. “Targeting MiR‑155‑5p and MiR‑221‑3p by Peptide Nucleic Acids Induces Caspase‑3 Activation and Apoptosis in Temozolomide‑resistant T98G Glioma Cells.” INTERNATIONAL JOURNAL OF ONCOLOGY 55 (1): 59–68.
Chicago author-date (all authors)
Milani, Roberta, Eleonora Brognara, Enrica Fabbri, Alex Manicardi, Roberto Corradini, Alessia Finotti, Jessica Gasparello, Monica Borgatti, Lucia Cosenza, Ilaria Lampronti, Maria Dechecchi, Giulio Cabrini, and Roberto Gambari. 2019. “Targeting MiR‑155‑5p and MiR‑221‑3p by Peptide Nucleic Acids Induces Caspase‑3 Activation and Apoptosis in Temozolomide‑resistant T98G Glioma Cells.” INTERNATIONAL JOURNAL OF ONCOLOGY 55 (1): 59–68.
Vancouver
1.
Milani R, Brognara E, Fabbri E, Manicardi A, Corradini R, Finotti A, et al. Targeting miR‑155‑5p and miR‑221‑3p by peptide nucleic acids induces caspase‑3 activation and apoptosis in temozolomide‑resistant T98G glioma cells. INTERNATIONAL JOURNAL OF ONCOLOGY. 2019;55(1):59–68.
IEEE
[1]
R. Milani et al., “Targeting miR‑155‑5p and miR‑221‑3p by peptide nucleic acids induces caspase‑3 activation and apoptosis in temozolomide‑resistant T98G glioma cells,” INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 55, no. 1, pp. 59–68, 2019.
@article{8646373,
  abstract     = {The present study investigated the effects of the combined treatment of two peptide nucleic acids (PNAs), directed against microRNAs involved in caspase-3 mRNA regulation (miR-155-5p and miR-221-3p) in the temozolomide (TMZ)-resistant T98G glioma cell line. These PNAs were conjugated with an octaarginine tail in order to obtain an efficient delivery to treated cells. The effects of singularly administered PNAs or a combined treatment with both PNAs were examined on apoptosis, with the aim to determine whether reversion of the drug-resistance phenotype was obtained. Specificity of the PNA-mediated effects was analyzed by reverse transcription-quantitative polymerase-chain reaction, which demonstrated that the effects of R8-PNA-a155 and R8-PNA-a221 anti-miR PNAs were specific. Furthermore, the results obtained confirmed that both PNAs induced apoptosis when used on the temozolomide-resistant T98G glioma cell line. Notably, co-administration of both anti-miR-155 and anti-miR-221 PNAs was associated with an increased proapoptotic activity. In addition, TMZ further increased the induction of apoptosis in T98G cells co-treated with anti-miR-155 and anti-miR-221 PNAs.},
  author       = {Milani, Roberta and Brognara, Eleonora and Fabbri, Enrica and Manicardi, Alex and Corradini, Roberto and Finotti, Alessia and Gasparello, Jessica and Borgatti, Monica and Cosenza, Lucia and Lampronti, Ilaria and Dechecchi, Maria and Cabrini, Giulio and Gambari, Roberto},
  issn         = {1019-6439},
  journal      = {INTERNATIONAL JOURNAL OF ONCOLOGY},
  keywords     = {peptide nucleic acids,glioma,microRNAs,miR-221-3p,miR-155-5p,miRNA targeting,delivery,apoptosis,temozolomide,GENE-EXPRESSION,BIOLOGICAL-ACTIVITY,MICRORNA FUNCTIONS,STEM-CELLS,GLIOBLASTOMA,CANCER,PNA,INHIBITION,GROWTH,DNA},
  language     = {eng},
  number       = {1},
  pages        = {59--68},
  title        = {Targeting miR‑155‑5p and miR‑221‑3p by peptide nucleic acids induces caspase‑3 activation and apoptosis in temozolomide‑resistant T98G glioma cells},
  url          = {http://dx.doi.org/10.3892/ijo.2019.4810},
  volume       = {55},
  year         = {2019},
}

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