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Physiological expression of miR-130a during differentiation of CD34+ human hematopoietic stem cells results in the inhibition of monocyte differentiation

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Abstract
MicroRNAs (miRNA) are small noncoding RNAs that regulate gene expression by targeting mRNAs in a sequence specific manner, thereby determining their degradation or inhibiting translation. They are involved in processes such as proliferation, differentiation and apoptosis by fine-tuning the expression of genes underlying such events. The expression of specific miRNAs is involved in hematopoietic differentiation and their deregulation contributes to the development of hematopoietic malignancies such as acute myeloid leukemia (AML). miR-130a is over-expressed in AML. Here we show that miR-130a is physiologically expressed in myeloblasts and down regulated during monocyte differentiation. Gain- and loss-of-function experiments performed on CD34(+) human hematopoietic stem cells confirmed that expression of miR-130a inhibits monocyte differentiation by interfering with the expression of key transcription factors HOXA10, IRF8, KLF4, MAFB and PU-1. The data obtained in this study highlight that the correct modulation of miR-130a is necessary for normal differentiation to occur and confirming that deregulation of this miRNA might underlie the differentiation block occurring in AML.
Keywords
Hematopoiesis, Differentiation, Stem cell, Monocyte, Acute myeloid leukemia, microRNA, Transcription factor, PEPTIDE NUCLEIC-ACIDS, ACUTE MYELOID-LEUKEMIA, MICRORNAS, MAFB, PATHWAY, TARGET, PNA

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MLA
Mammoli, Fabiana, et al. “Physiological Expression of MiR-130a during Differentiation of CD34+ Human Hematopoietic Stem Cells Results in the Inhibition of Monocyte Differentiation.” EXPERIMENTAL CELL RESEARCH, vol. 382, no. 1, 2019.
APA
Mammoli, F., Parenti, S., Lomiento, M., Gemelli, C., Atene, C. G., Grande, A., … Ferrari, S. (2019). Physiological expression of miR-130a during differentiation of CD34+ human hematopoietic stem cells results in the inhibition of monocyte differentiation. EXPERIMENTAL CELL RESEARCH, 382(1).
Chicago author-date
Mammoli, Fabiana, Sandra Parenti, Mariana Lomiento, Claudia Gemelli, Claudio Giacinto Atene, Alexis Grande, Roberto Corradini, et al. 2019. “Physiological Expression of MiR-130a during Differentiation of CD34+ Human Hematopoietic Stem Cells Results in the Inhibition of Monocyte Differentiation.” EXPERIMENTAL CELL RESEARCH 382 (1).
Chicago author-date (all authors)
Mammoli, Fabiana, Sandra Parenti, Mariana Lomiento, Claudia Gemelli, Claudio Giacinto Atene, Alexis Grande, Roberto Corradini, Alex Manicardi, Sebastian Fantini, Tommaso Zanocco-Marani, and Sergio Ferrari. 2019. “Physiological Expression of MiR-130a during Differentiation of CD34+ Human Hematopoietic Stem Cells Results in the Inhibition of Monocyte Differentiation.” EXPERIMENTAL CELL RESEARCH 382 (1).
Vancouver
1.
Mammoli F, Parenti S, Lomiento M, Gemelli C, Atene CG, Grande A, et al. Physiological expression of miR-130a during differentiation of CD34+ human hematopoietic stem cells results in the inhibition of monocyte differentiation. EXPERIMENTAL CELL RESEARCH. 2019;382(1).
IEEE
[1]
F. Mammoli et al., “Physiological expression of miR-130a during differentiation of CD34+ human hematopoietic stem cells results in the inhibition of monocyte differentiation,” EXPERIMENTAL CELL RESEARCH, vol. 382, no. 1, 2019.
@article{8646371,
  abstract     = {MicroRNAs (miRNA) are small noncoding RNAs that regulate gene expression by targeting mRNAs in a sequence specific manner, thereby determining their degradation or inhibiting translation. They are involved in processes such as proliferation, differentiation and apoptosis by fine-tuning the expression of genes underlying such events. The expression of specific miRNAs is involved in hematopoietic differentiation and their deregulation contributes to the development of hematopoietic malignancies such as acute myeloid leukemia (AML). miR-130a is over-expressed in AML. Here we show that miR-130a is physiologically expressed in myeloblasts and down regulated during monocyte differentiation. Gain- and loss-of-function experiments performed on CD34(+) human hematopoietic stem cells confirmed that expression of miR-130a inhibits monocyte differentiation by interfering with the expression of key transcription factors HOXA10, IRF8, KLF4, MAFB and PU-1. The data obtained in this study highlight that the correct modulation of miR-130a is necessary for normal differentiation to occur and confirming that deregulation of this miRNA might underlie the differentiation block occurring in AML.},
  articleno    = {111445},
  author       = {Mammoli, Fabiana and Parenti, Sandra and Lomiento, Mariana and Gemelli, Claudia and Atene, Claudio Giacinto and Grande, Alexis and Corradini, Roberto and Manicardi, Alex and Fantini, Sebastian and Zanocco-Marani, Tommaso and Ferrari, Sergio},
  issn         = {0014-4827},
  journal      = {EXPERIMENTAL CELL RESEARCH},
  keywords     = {Hematopoiesis,Differentiation,Stem cell,Monocyte,Acute myeloid leukemia,microRNA,Transcription factor,PEPTIDE NUCLEIC-ACIDS,ACUTE MYELOID-LEUKEMIA,MICRORNAS,MAFB,PATHWAY,TARGET,PNA},
  language     = {eng},
  number       = {1},
  pages        = {7},
  title        = {Physiological expression of miR-130a during differentiation of CD34+ human hematopoietic stem cells results in the inhibition of monocyte differentiation},
  url          = {http://dx.doi.org/10.1016/j.yexcr.2019.05.026},
  volume       = {382},
  year         = {2019},
}

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