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Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas

(2019) PLANT AND SOIL. 441(1-2). p.485-497
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Abstract
Aim: A growing number of studies show a discrepancy between the isotopic composition of xylem water and plant water sources. We tested the effect of arbuscular mycorrhizal fungi (AMF) on the isotopic composition of Acacia caven xylem water. As the most common plant-fungal association, AMF might explain this isotopic mismatch. Methods: Seedlings were grown with and without AMF and irrigated with the same water. After 120 days, stem and soil samples were collected and following cryogenic distillation, H and O isotopic composition of xylem and soil water, as well as irrigation water, was measured. Results: Xylem water of non-mycorrhizal seedlings was significantly depleted in H-2 compared to soil water (differences up to -15.6 parts per thousand). When AMF were present, the depletion was significantly higher and appeared for both H and O (differences up to -24.6 parts per thousand for delta H-2 and - 2.9 parts per thousand for delta O-18 between soil and xylem water). Conclusions: Results suggest that isotopic fractionation occurred during water uptake in this xerophytic species. To explain this, we propose an aquaporin-driven mechanism mediating water transport via transmembrane passage. Furthermore, we show for the first time, that AMF enhance the observed discrimination against heavy isotopes, probably by enforcing water passage through aquaporins. Given their ubiquity, AMF could question the fractionation-free assumption during root water uptake.
Keywords
Aquaporins, Ecohydrology, Isotope fractionation, Mycorrhiza, Plant-soil-water interactions, Stable isotopes, SOIL-WATER, ECOHYDROLOGICAL SEPARATION, DROUGHT STRESS, PLANT, FUNGI, NITROGEN, EXTRACTION, ECTOMYCORRHIZAL, SYMBIOSIS, FOREST

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Citation

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MLA
Poca, María, et al. “Isotope Fractionation during Root Water Uptake by Acacia Caven Is Enhanced by Arbuscular Mycorrhizas.” PLANT AND SOIL, vol. 441, no. 1–2, 2019, pp. 485–97.
APA
Poca, M., Coomans de Brachène, O., Urcelay, C., Zeballos, S. R., Bodé, S., & Boeckx, P. (2019). Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas. PLANT AND SOIL, 441(1–2), 485–497.
Chicago author-date
Poca, María, Olivia Coomans de Brachène, Carlos Urcelay, Sebastián R Zeballos, Samuel Bodé, and Pascal Boeckx. 2019. “Isotope Fractionation during Root Water Uptake by Acacia Caven Is Enhanced by Arbuscular Mycorrhizas.” PLANT AND SOIL 441 (1–2): 485–97.
Chicago author-date (all authors)
Poca, María, Olivia Coomans de Brachène, Carlos Urcelay, Sebastián R Zeballos, Samuel Bodé, and Pascal Boeckx. 2019. “Isotope Fractionation during Root Water Uptake by Acacia Caven Is Enhanced by Arbuscular Mycorrhizas.” PLANT AND SOIL 441 (1–2): 485–497.
Vancouver
1.
Poca M, Coomans de Brachène O, Urcelay C, Zeballos SR, Bodé S, Boeckx P. Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas. PLANT AND SOIL. 2019;441(1–2):485–97.
IEEE
[1]
M. Poca, O. Coomans de Brachène, C. Urcelay, S. R. Zeballos, S. Bodé, and P. Boeckx, “Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas,” PLANT AND SOIL, vol. 441, no. 1–2, pp. 485–497, 2019.
@article{8644835,
  abstract     = {Aim: A growing number of studies show a discrepancy between the isotopic composition of xylem water and plant water sources. We tested the effect of arbuscular mycorrhizal fungi (AMF) on the isotopic composition of Acacia caven xylem water. As the most common plant-fungal association, AMF might explain this isotopic mismatch.
Methods: Seedlings were grown with and without AMF and irrigated with the same water. After 120 days, stem and soil samples were collected and following cryogenic distillation, H and O isotopic composition of xylem and soil water, as well as irrigation water, was measured.
Results: Xylem water of non-mycorrhizal seedlings was significantly depleted in H-2 compared to soil water (differences up to -15.6 parts per thousand). When AMF were present, the depletion was significantly higher and appeared for both H and O (differences up to -24.6 parts per thousand for delta H-2 and - 2.9 parts per thousand for delta O-18 between soil and xylem water).
Conclusions: Results suggest that isotopic fractionation occurred during water uptake in this xerophytic species. To explain this, we propose an aquaporin-driven mechanism mediating water transport via transmembrane passage. Furthermore, we show for the first time, that AMF enhance the observed discrimination against heavy isotopes, probably by enforcing water passage through aquaporins. Given their ubiquity, AMF could question the fractionation-free assumption during root water uptake.},
  author       = {Poca, María and Coomans de Brachène, Olivia and Urcelay, Carlos and Zeballos, Sebastián R and Bodé, Samuel and Boeckx, Pascal},
  issn         = {0032-079X},
  journal      = {PLANT AND SOIL},
  keywords     = {Aquaporins,Ecohydrology,Isotope fractionation,Mycorrhiza,Plant-soil-water interactions,Stable isotopes,SOIL-WATER,ECOHYDROLOGICAL SEPARATION,DROUGHT STRESS,PLANT,FUNGI,NITROGEN,EXTRACTION,ECTOMYCORRHIZAL,SYMBIOSIS,FOREST},
  language     = {eng},
  number       = {1-2},
  pages        = {485--497},
  title        = {Isotope fractionation during root water uptake by Acacia caven is enhanced by arbuscular mycorrhizas},
  url          = {http://dx.doi.org/10.1007/s11104-019-04139-1},
  volume       = {441},
  year         = {2019},
}

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