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Self-pressurised rapid freezing : a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes

(2020) NEMATOLOGY. 22(1). p.103-110
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Abstract
Ultrastructural analysis of the early development of nematodes is hampered by the impermeability of the eggshell to most commonly used fixatives. High-pressure freezing (HPF), a physical cryo-fixation method, facilitates a fast rate of fixation. and by using this method the issue of the uneven delivery of fixative is circumvented. Although HPF results in a superior preservation of the fine structure, the equipment costs impede a wider application of this method. Self-pressurised rapid freezing (SPRF) is an alternative low-cost cryo-fixation method, and its usefulness was evaluated in an ultrastructural study of the eggshell and the cuticle of unhatched second-stage juveniles (J2) of Globodera rostochiensis and Heterodera schachtii. A comparison with conventional (chemical) fixation demonstrates that SPRF fixation results in a remarkably well-preserved ultrastructure of the entire egg including both the eggshell and the cellular details of the unhatched J2. Therefore. SPRF fixation is proposed as an affordable. relatively easy-to-use and time-efficient technique to study the ultrastructure of unhatched J2 and eggs of nematodes.
Keywords
Agronomy and Crop Science, Ecology, Evolution, Behavior and Systematics

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MLA
Claeys, Myriam, et al. “Self-Pressurised Rapid Freezing : A Time-Efficient and Affordable Cryo-Fixation Method for Ultrastructural Observations on Unhatched Cyst Nematodes.” NEMATOLOGY, vol. 22, no. 1, 2020, pp. 103–10.
APA
Claeys, M., Handayani, N. D., Yushin, V. V., Lestari, P., Dikin, A., Helder, J., & Bert, W. (2020). Self-pressurised rapid freezing : a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes. NEMATOLOGY, 22(1), 103–110.
Chicago author-date
Claeys, Myriam, Nurul Dwi Handayani, Vladimir V. Yushin, Prabowo Lestari, Antarjo Dikin, Johannes Helder, and Wim Bert. 2020. “Self-Pressurised Rapid Freezing : A Time-Efficient and Affordable Cryo-Fixation Method for Ultrastructural Observations on Unhatched Cyst Nematodes.” NEMATOLOGY 22 (1): 103–10.
Chicago author-date (all authors)
Claeys, Myriam, Nurul Dwi Handayani, Vladimir V. Yushin, Prabowo Lestari, Antarjo Dikin, Johannes Helder, and Wim Bert. 2020. “Self-Pressurised Rapid Freezing : A Time-Efficient and Affordable Cryo-Fixation Method for Ultrastructural Observations on Unhatched Cyst Nematodes.” NEMATOLOGY 22 (1): 103–110.
Vancouver
1.
Claeys M, Handayani ND, Yushin VV, Lestari P, Dikin A, Helder J, et al. Self-pressurised rapid freezing : a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes. NEMATOLOGY. 2020;22(1):103–10.
IEEE
[1]
M. Claeys et al., “Self-pressurised rapid freezing : a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes,” NEMATOLOGY, vol. 22, no. 1, pp. 103–110, 2020.
@article{8644430,
  abstract     = {Ultrastructural analysis of the early development of nematodes is hampered by the impermeability of the eggshell to most commonly used fixatives. High-pressure freezing (HPF), a physical cryo-fixation method, facilitates a fast rate of fixation. and by using this method the issue of the uneven delivery of fixative is circumvented. Although HPF results in a superior preservation of the fine structure, the equipment costs impede a wider application of this method. Self-pressurised rapid freezing (SPRF) is an alternative low-cost cryo-fixation method, and its usefulness was evaluated in an ultrastructural study of the eggshell and the cuticle of unhatched second-stage juveniles (J2) of Globodera rostochiensis and Heterodera schachtii. A comparison with conventional (chemical) fixation demonstrates that SPRF fixation results in a remarkably well-preserved ultrastructure of the entire egg including both the eggshell and the cellular details of the unhatched J2. Therefore. SPRF fixation is proposed as an affordable. relatively easy-to-use and time-efficient technique to study the ultrastructure of unhatched J2 and eggs of nematodes.},
  author       = {Claeys, Myriam and Handayani, Nurul Dwi and Yushin, Vladimir V. and Lestari, Prabowo and Dikin, Antarjo and Helder, Johannes and Bert, Wim},
  issn         = {1388-5545},
  journal      = {NEMATOLOGY},
  keywords     = {Agronomy and Crop Science,Ecology,Evolution,Behavior and Systematics},
  language     = {eng},
  number       = {1},
  pages        = {103--110},
  title        = {Self-pressurised rapid freezing : a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes},
  url          = {http://dx.doi.org/10.1163/15685411-00003287},
  volume       = {22},
  year         = {2020},
}

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