Advanced search
1 file | 3.99 MB Add to list

Small RNA NcS27 co-regulates utilization of carbon sources in Burkholderia cenocepacia J2315

Andrea Sass (UGent) , Stijn De Waele (UGent) , Simon Daled (UGent) , Bart Devreese (UGent) , Dieter Deforce (UGent) , Filip Van Nieuwerburgh (UGent) and Tom Coenye (UGent)
(2019) MICROBIOLOGY-SGM. 165(10). p.1135-1150
Author
Organization
Abstract
Small non-coding sRNAs have versatile roles in regulating bacterial metabolism. Four short homologous Burkholderia cenocepacia sRNAs strongly expressed under conditions of growth arrest were recently identified. Here we report the detailed investigation of one of these, NcS27. sRNA NcS27 contains a short putative target recognition sequence, which is conserved throughout the order Burkholderiales. This sequence is the reverse complement of the Shine-Dalgarno sequence of a large number of genes involved in transport and metabolism of amino acids and carbohydrates. Overexpression of NcS27 sRNA had a distinct impact on growth, attenuating growth on a variety of substrates such as phenylalanine, tyrosine, glycerol and galactose, while having no effect on growth on other substrates. Transcriptomics and proteomics of NcS27 overexpression and silencing mutants revealed numerous predicted targets changing expression, notably of genes involved in degradation of aromatic amino acids phenylalanine and tyrosine, and in transport of carbohydrates. The conserved target recognition sequence was essential for growth phenotypes and gene expression changes. Cumulatively, our data point to a role of NcS27 in regulating the shutdown of metabolism upon nutrient deprivation in B. cenocepacia. We propose Burkholderia double-hairpin sRNA regulator bdhR1 as designation for ncS27.
Keywords
Burkholderia cenocepacia, sRNA, RNA-Seq, phenylalanine degradation, ABC transporter, TARGET MESSENGER-RNAS, REGULATORY RNAS, CATABOLIC PATHWAY, ACID, HFQ, DEGRADATION, ROLES, RECOGNITION, DISCOVERY, PATHOGEN

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.99 MB

Citation

Please use this url to cite or link to this publication:

MLA
Sass, Andrea, et al. “Small RNA NcS27 Co-Regulates Utilization of Carbon Sources in Burkholderia Cenocepacia J2315.” MICROBIOLOGY-SGM, vol. 165, no. 10, 2019, pp. 1135–50.
APA
Sass, A., De Waele, S., Daled, S., Devreese, B., Deforce, D., Van Nieuwerburgh, F., & Coenye, T. (2019). Small RNA NcS27 co-regulates utilization of carbon sources in Burkholderia cenocepacia J2315. MICROBIOLOGY-SGM, 165(10), 1135–1150.
Chicago author-date
Sass, Andrea, Stijn De Waele, Simon Daled, Bart Devreese, Dieter Deforce, Filip Van Nieuwerburgh, and Tom Coenye. 2019. “Small RNA NcS27 Co-Regulates Utilization of Carbon Sources in Burkholderia Cenocepacia J2315.” MICROBIOLOGY-SGM 165 (10): 1135–50.
Chicago author-date (all authors)
Sass, Andrea, Stijn De Waele, Simon Daled, Bart Devreese, Dieter Deforce, Filip Van Nieuwerburgh, and Tom Coenye. 2019. “Small RNA NcS27 Co-Regulates Utilization of Carbon Sources in Burkholderia Cenocepacia J2315.” MICROBIOLOGY-SGM 165 (10): 1135–1150.
Vancouver
1.
Sass A, De Waele S, Daled S, Devreese B, Deforce D, Van Nieuwerburgh F, et al. Small RNA NcS27 co-regulates utilization of carbon sources in Burkholderia cenocepacia J2315. MICROBIOLOGY-SGM. 2019;165(10):1135–50.
IEEE
[1]
A. Sass et al., “Small RNA NcS27 co-regulates utilization of carbon sources in Burkholderia cenocepacia J2315,” MICROBIOLOGY-SGM, vol. 165, no. 10, pp. 1135–1150, 2019.
@article{8643450,
  abstract     = {Small non-coding sRNAs have versatile roles in regulating bacterial metabolism. Four short homologous Burkholderia cenocepacia sRNAs strongly expressed under conditions of growth arrest were recently identified. Here we report the detailed investigation of one of these, NcS27. sRNA NcS27 contains a short putative target recognition sequence, which is conserved throughout the order Burkholderiales. This sequence is the reverse complement of the Shine-Dalgarno sequence of a large number of genes involved in transport and metabolism of amino acids and carbohydrates. Overexpression of NcS27 sRNA had a distinct impact on growth, attenuating growth on a variety of substrates such as phenylalanine, tyrosine, glycerol and galactose, while having no effect on growth on other substrates. Transcriptomics and proteomics of NcS27 overexpression and silencing mutants revealed numerous predicted targets changing expression, notably of genes involved in degradation of aromatic amino acids phenylalanine and tyrosine, and in transport of carbohydrates. The conserved target recognition sequence was essential for growth phenotypes and gene expression changes. Cumulatively, our data point to a role of NcS27 in regulating the shutdown of metabolism upon nutrient deprivation in B. cenocepacia. We propose Burkholderia double-hairpin sRNA regulator bdhR1 as designation for ncS27.},
  author       = {Sass, Andrea and De Waele, Stijn and Daled, Simon and Devreese, Bart and Deforce, Dieter and Van Nieuwerburgh, Filip and Coenye, Tom},
  issn         = {1350-0872},
  journal      = {MICROBIOLOGY-SGM},
  keywords     = {Burkholderia cenocepacia,sRNA,RNA-Seq,phenylalanine degradation,ABC transporter,TARGET MESSENGER-RNAS,REGULATORY RNAS,CATABOLIC PATHWAY,ACID,HFQ,DEGRADATION,ROLES,RECOGNITION,DISCOVERY,PATHOGEN},
  language     = {eng},
  number       = {10},
  pages        = {1135--1150},
  title        = {Small RNA NcS27 co-regulates utilization of carbon sources in Burkholderia cenocepacia J2315},
  url          = {http://dx.doi.org/10.1099/mic.0.000848},
  volume       = {165},
  year         = {2019},
}

Altmetric
View in Altmetric
Web of Science
Times cited: