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5′ untranslated regions : the next regulatory sequence in yeast synthetic biology

Yatti De Nijs (UGent) , Sofie De Maeseneire (UGent) and Wim Soetaert (UGent)
(2020) BIOLOGICAL REVIEWS. 95(2). p.517-529
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Abstract
When developing industrial biotechnology processes, Saccharomyces cerevisiae (baker's yeast or brewer's yeast) is a popular choice as a microbial host. Many tools have been developed in the fields of synthetic biology and metabolic engineering to introduce heterologous pathways and tune their expression in yeast. Such tools mainly focus on controlling transcription, whereas post-transcriptional regulation is often overlooked. Herein we discuss regulatory elements found in the 5 ' untranslated region (UTR) and their influence on protein synthesis. We provide not only an overall picture, but also a set of design rules on how to engineer a 5 ' UTR. The reader is also referred to currently available models that allow gene expression to be tuned predictably using different 5 ' UTRs.
Keywords
General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences, protein synthesis, post-transcriptional regulation, 5 ' UTR, regulatory elements, Saccharomyces cerevisiae, OPEN READING FRAMES, CAP-INDEPENDENT TRANSLATION, MESSENGER-RNA STRUCTURES, GENOME-WIDE MEASUREMENT, RIBOSOME ENTRY SITES, SACCHAROMYCES-CEREVISIAE, GENE-EXPRESSION, SECONDARY STRUCTURE, START SITES, INTERNAL INITIATION

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MLA
De Nijs, Yatti, et al. “5′ Untranslated Regions : The next Regulatory Sequence in Yeast Synthetic Biology.” BIOLOGICAL REVIEWS, vol. 95, no. 2, 2020, pp. 517–29.
APA
De Nijs, Y., De Maeseneire, S., & Soetaert, W. (2020). 5′ untranslated regions : the next regulatory sequence in yeast synthetic biology. BIOLOGICAL REVIEWS, 95(2), 517–529.
Chicago author-date
De Nijs, Yatti, Sofie De Maeseneire, and Wim Soetaert. 2020. “5′ Untranslated Regions : The next Regulatory Sequence in Yeast Synthetic Biology.” BIOLOGICAL REVIEWS 95 (2): 517–29.
Chicago author-date (all authors)
De Nijs, Yatti, Sofie De Maeseneire, and Wim Soetaert. 2020. “5′ Untranslated Regions : The next Regulatory Sequence in Yeast Synthetic Biology.” BIOLOGICAL REVIEWS 95 (2): 517–529.
Vancouver
1.
De Nijs Y, De Maeseneire S, Soetaert W. 5′ untranslated regions : the next regulatory sequence in yeast synthetic biology. BIOLOGICAL REVIEWS. 2020;95(2):517–29.
IEEE
[1]
Y. De Nijs, S. De Maeseneire, and W. Soetaert, “5′ untranslated regions : the next regulatory sequence in yeast synthetic biology,” BIOLOGICAL REVIEWS, vol. 95, no. 2, pp. 517–529, 2020.
@article{8642645,
  abstract     = {{When developing industrial biotechnology processes, Saccharomyces cerevisiae (baker's yeast or brewer's yeast) is a popular choice as a microbial host. Many tools have been developed in the fields of synthetic biology and metabolic engineering to introduce heterologous pathways and tune their expression in yeast. Such tools mainly focus on controlling transcription, whereas post-transcriptional regulation is often overlooked. Herein we discuss regulatory elements found in the 5 ' untranslated region (UTR) and their influence on protein synthesis. We provide not only an overall picture, but also a set of design rules on how to engineer a 5 ' UTR. The reader is also referred to currently available models that allow gene expression to be tuned predictably using different 5 ' UTRs.}},
  author       = {{De Nijs, Yatti and De Maeseneire, Sofie and Soetaert, Wim}},
  issn         = {{1464-7931}},
  journal      = {{BIOLOGICAL REVIEWS}},
  keywords     = {{General Biochemistry,Genetics and Molecular Biology,General Agricultural and Biological Sciences,protein synthesis,post-transcriptional regulation,5 ' UTR,regulatory elements,Saccharomyces cerevisiae,OPEN READING FRAMES,CAP-INDEPENDENT TRANSLATION,MESSENGER-RNA STRUCTURES,GENOME-WIDE MEASUREMENT,RIBOSOME ENTRY SITES,SACCHAROMYCES-CEREVISIAE,GENE-EXPRESSION,SECONDARY STRUCTURE,START SITES,INTERNAL INITIATION}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{517--529}},
  title        = {{5′ untranslated regions : the next regulatory sequence in yeast synthetic biology}},
  url          = {{http://dx.doi.org/10.1111/brv.12575}},
  volume       = {{95}},
  year         = {{2020}},
}

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