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Molecular and genetic analysis of resistance to METI-I acaricides in Iranian populations of the citrus red mite Panonychus citri

Elaheh Shafiei Alavijeh, Jahangir Khajehali, Simon Snoeck, Rafaela Panteleri, Mohammad Ghadamyari, Wim Jonckheere (UGent) , Sabina Bajda-Wybouw (UGent) , Corinna Saalwaechter, Sven Geibel, Vassilis Douris, et al.
(2020) PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY. 164. p.73-84
Author
Organization
Project
  • POLYADAPT (Molecular-genetic mechanisms of extreme adaptation in a polyphagous agricultural pest)
  • SuperPests (Innovative tools for rational control of the most difficult-to-manage pests (super pests) and the diseases they transmit)
Abstract
The citrus red mite, Panonychus citri, is a major pest on citrus all around the world. Mitochondrial Electron Transport Inhibitors of complex I (METI-I) acaricides such as fenpyroximate have been used extensively to control P. citri populations, which resulted in multiple reports of METI-I resistant populations in the field. In this study, biochemical and molecular mechanisms of fenpyroximate resistance were investigated in P. citri. Seven populations were collected from Northern provinces of Iran. Resistance ratios were determined and reached up to 75-fold in comparison to a fenpyroximate susceptible population. Cross-resistance to two additional METI-I acaricides, pyridaben and tebufenpyrad, was detected. PBO synergism experiments, in vivo enzyme assays and gene expression analysis suggest a minor involvement of cytochrome P450 monooxygenases in fenpyroximate resistance, which is in contrast with many reported cases for the closely related Tetranychus urticae. Next, we determined the frequency of a well-known mutation in the target-site of METI-Is, the PSST subunit, associated with METI-I resistance. Indeed, the H92R substitution was detected in a highly fenpyroximate resistant P. citri population. Additionally, a new amino acid substitution at a conserved site in the PSST subunit was detected, A94V, with higher allele frequencies in a moderately resistant population. Marker-assisted back-crossing in a susceptible background confirmed the potential involvement of the newly discovered A94V mutation in fenpyroximate resistance. However, introduction of the A94V mutation in the PSST homologue of D. melanogaster using CRISPR-Cas9 did not result in fenpyroximate resistant flies. In addition, differences in binding curves between METI-Is and complex I measured directly, in isolated transgenic and wildtype mitochondria preparations, could not be found.
Keywords
Agronomy and Crop Science, Health, Toxicology and Mutagenesis, General Medicine, Citrus red mite, Fenpyroximate, METI-I, Drosophila, CRISPR-Cas9, Target-site resistance, NDUFS7, TETRANYCHUS-URTICAE ACARI, NADH-UBIQUINONE OXIDOREDUCTASE, SULFUR CLUSTER N2, TARGET-SITE RESISTANCE, COMPLEX-I, CROSS-RESISTANCE, KOCH ACARI, SODIUM-CHANNEL, INHIBITORS, PROTEIN

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Citation

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MLA
Alavijeh, Elaheh Shafiei, et al. “Molecular and Genetic Analysis of Resistance to METI-I Acaricides in Iranian Populations of the Citrus Red Mite Panonychus Citri.” PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, vol. 164, 2020, pp. 73–84, doi:10.1016/j.pestbp.2019.12.009.
APA
Alavijeh, E. S., Khajehali, J., Snoeck, S., Panteleri, R., Ghadamyari, M., Jonckheere, W., … Dermauw, W. (2020). Molecular and genetic analysis of resistance to METI-I acaricides in Iranian populations of the citrus red mite Panonychus citri. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 164, 73–84. https://doi.org/10.1016/j.pestbp.2019.12.009
Chicago author-date
Alavijeh, Elaheh Shafiei, Jahangir Khajehali, Simon Snoeck, Rafaela Panteleri, Mohammad Ghadamyari, Wim Jonckheere, Sabina Bajda-Wybouw, et al. 2020. “Molecular and Genetic Analysis of Resistance to METI-I Acaricides in Iranian Populations of the Citrus Red Mite Panonychus Citri.” PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 164: 73–84. https://doi.org/10.1016/j.pestbp.2019.12.009.
Chicago author-date (all authors)
Alavijeh, Elaheh Shafiei, Jahangir Khajehali, Simon Snoeck, Rafaela Panteleri, Mohammad Ghadamyari, Wim Jonckheere, Sabina Bajda-Wybouw, Corinna Saalwaechter, Sven Geibel, Vassilis Douris, John Vontas, Thomas Van Leeuwen, and Wannes Dermauw. 2020. “Molecular and Genetic Analysis of Resistance to METI-I Acaricides in Iranian Populations of the Citrus Red Mite Panonychus Citri.” PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 164: 73–84. doi:10.1016/j.pestbp.2019.12.009.
Vancouver
1.
Alavijeh ES, Khajehali J, Snoeck S, Panteleri R, Ghadamyari M, Jonckheere W, et al. Molecular and genetic analysis of resistance to METI-I acaricides in Iranian populations of the citrus red mite Panonychus citri. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY. 2020;164:73–84.
IEEE
[1]
E. S. Alavijeh et al., “Molecular and genetic analysis of resistance to METI-I acaricides in Iranian populations of the citrus red mite Panonychus citri,” PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, vol. 164, pp. 73–84, 2020.
@article{8640665,
  abstract     = {{The citrus red mite, Panonychus citri, is a major pest on citrus all around the world. Mitochondrial Electron Transport Inhibitors of complex I (METI-I) acaricides such as fenpyroximate have been used extensively to control P. citri populations, which resulted in multiple reports of METI-I resistant populations in the field. In this study, biochemical and molecular mechanisms of fenpyroximate resistance were investigated in P. citri. Seven populations were collected from Northern provinces of Iran. Resistance ratios were determined and reached up to 75-fold in comparison to a fenpyroximate susceptible population. Cross-resistance to two additional METI-I acaricides, pyridaben and tebufenpyrad, was detected. PBO synergism experiments, in vivo enzyme assays and gene expression analysis suggest a minor involvement of cytochrome P450 monooxygenases in fenpyroximate resistance, which is in contrast with many reported cases for the closely related Tetranychus urticae. Next, we determined the frequency of a well-known mutation in the target-site of METI-Is, the PSST subunit, associated with METI-I resistance. Indeed, the H92R substitution was detected in a highly fenpyroximate resistant P. citri population. Additionally, a new amino acid substitution at a conserved site in the PSST subunit was detected, A94V, with higher allele frequencies in a moderately resistant population. Marker-assisted back-crossing in a susceptible background confirmed the potential involvement of the newly discovered A94V mutation in fenpyroximate resistance. However, introduction of the A94V mutation in the PSST homologue of D. melanogaster using CRISPR-Cas9 did not result in fenpyroximate resistant flies. In addition, differences in binding curves between METI-Is and complex I measured directly, in isolated transgenic and wildtype mitochondria preparations, could not be found.}},
  author       = {{Alavijeh, Elaheh Shafiei and Khajehali, Jahangir and Snoeck, Simon and Panteleri, Rafaela and Ghadamyari, Mohammad and Jonckheere, Wim and Bajda-Wybouw, Sabina and Saalwaechter, Corinna and Geibel, Sven and Douris, Vassilis and Vontas, John and Van Leeuwen, Thomas and Dermauw, Wannes}},
  issn         = {{0048-3575}},
  journal      = {{PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY}},
  keywords     = {{Agronomy and Crop Science,Health,Toxicology and Mutagenesis,General Medicine,Citrus red mite,Fenpyroximate,METI-I,Drosophila,CRISPR-Cas9,Target-site resistance,NDUFS7,TETRANYCHUS-URTICAE ACARI,NADH-UBIQUINONE OXIDOREDUCTASE,SULFUR CLUSTER N2,TARGET-SITE RESISTANCE,COMPLEX-I,CROSS-RESISTANCE,KOCH ACARI,SODIUM-CHANNEL,INHIBITORS,PROTEIN}},
  language     = {{eng}},
  pages        = {{73--84}},
  title        = {{Molecular and genetic analysis of resistance to METI-I acaricides in Iranian populations of the citrus red mite Panonychus citri}},
  url          = {{http://dx.doi.org/10.1016/j.pestbp.2019.12.009}},
  volume       = {{164}},
  year         = {{2020}},
}
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