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Renal tubular epithelial cells add value in the diagnosis of upper urinary tract pathology

Matthijs Oyaert (UGent) , Marijn Speeckaert (UGent) , Jerina Boelens (UGent) and Joris Delanghe (UGent)
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Abstract
Background: Diagnosis of upper urinary tract infections (UTI) is challenging. We evaluated the analytical and diagnostic performance characteristics of renal tubular epithelial cells (RTECs) and transitional epithelial cells (TECs) on the Sysmex UF-5000 urine sediment analyzer. Methods: Urinary samples from 506 patients presenting with symptoms of a UTI were collected. Only samples for which a urinary culture was available were included. Analytical (imprecision, accuracy, stability and correlation with manual microscopy) and diagnostic performance (sensitivity and specificity) were evaluated. Results: The Sysmex UF-5000 demonstrated a good analytical performance. Depending on the storage time, storage conditions (2–8 °C or 20–25 °C) and urinary pH, RTECs and TECs were stable in urine for at least 4 h. Using Passing-Bablok and Bland-Altman analysis, an acceptable agreement was observed between the manual and automated methods. Compared to TECs, RTECs demonstrated an acceptable diagnostic performance for the diagnosis of upper UTI. Conclusions: While TECs do not seem to serve as a helpful marker, increased urinary levels of RTECs add value in the diagnosis of upper UTI and may be helpful in the discrimination between upper and lower UTIs.

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MLA
Oyaert, Matthijs, et al. “Renal Tubular Epithelial Cells Add Value in the Diagnosis of Upper Urinary Tract Pathology.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2020.
APA
Oyaert, M., Speeckaert, M., Boelens, J., & Delanghe, J. (2020). Renal tubular epithelial cells add value in the diagnosis of upper urinary tract pathology. CLINICAL CHEMISTRY AND LABORATORY MEDICINE.
Chicago author-date
Oyaert, Matthijs, Marijn Speeckaert, Jerina Boelens, and Joris Delanghe. 2020. “Renal Tubular Epithelial Cells Add Value in the Diagnosis of Upper Urinary Tract Pathology.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE.
Chicago author-date (all authors)
Oyaert, Matthijs, Marijn Speeckaert, Jerina Boelens, and Joris Delanghe. 2020. “Renal Tubular Epithelial Cells Add Value in the Diagnosis of Upper Urinary Tract Pathology.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE.
Vancouver
1.
Oyaert M, Speeckaert M, Boelens J, Delanghe J. Renal tubular epithelial cells add value in the diagnosis of upper urinary tract pathology. CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 2020;
IEEE
[1]
M. Oyaert, M. Speeckaert, J. Boelens, and J. Delanghe, “Renal tubular epithelial cells add value in the diagnosis of upper urinary tract pathology,” CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2020.
@article{8640585,
  abstract     = {Background: Diagnosis of upper urinary tract infections (UTI) is challenging. We evaluated the analytical and diagnostic performance characteristics of renal tubular epithelial cells (RTECs) and transitional epithelial cells (TECs) on the Sysmex UF-5000 urine sediment analyzer.
Methods: Urinary samples from 506 patients presenting with symptoms of a UTI were collected. Only samples for which a urinary culture was available were included. Analytical (imprecision, accuracy, stability and correlation with manual microscopy) and diagnostic performance (sensitivity and specificity) were evaluated.
Results: The Sysmex UF-5000 demonstrated a good analytical performance. Depending on the storage time, storage conditions (2–8 °C or 20–25 °C) and urinary pH, RTECs and TECs were stable in urine for at least 4 h. Using Passing-Bablok and Bland-Altman analysis, an acceptable agreement was observed between the manual and automated methods. Compared to TECs, RTECs demonstrated an acceptable diagnostic performance for the diagnosis of upper UTI.
Conclusions: While TECs do not seem to serve as a helpful marker, increased urinary levels of RTECs add value in the diagnosis of upper UTI and may be helpful in the discrimination between upper and lower UTIs.},
  author       = {Oyaert, Matthijs and Speeckaert, Marijn and Boelens, Jerina and Delanghe, Joris},
  issn         = {1434-6621},
  journal      = {CLINICAL CHEMISTRY AND LABORATORY MEDICINE},
  language     = {eng},
  title        = {Renal tubular epithelial cells add value in the diagnosis of upper urinary tract pathology},
  url          = {http://dx.doi.org/10.1515/cclm-2019-1068},
  year         = {2020},
}

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