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Mycolicibacterium smegmatis, basonym Mycobacterium smegmatis, causing pyogranulomatous mastitis and its cross-reactivity in bovine (para)tuberculosis testing

(2019) JOURNAL OF DAIRY SCIENCE. 102(9). p.8405-8409
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Abstract
Different mycobacterial species are encountered in bovine medicine. The fastidiously growing mycobacteria (Mycobacterium bovis as the cause of bovine tuberculosis, and Mycobacterium avium ssp. paratuberculosis, MAP, as the cause of paratuberculosis) are well known and targeted in eradication/control or monitoring programs in different countries, whereas the rapidly growing species is only rarely identified from bovine disease. The latter have occasionally been reported as the cause of bovine clinical mastitis, but recent reports are scarce. In this study, Mycolicibacterium smegmatis (basonym Mycobacterium smegmatis) was identified as cause of granulomatous, relapsing clinical mastitis in 2 cows from one Belgian dairy herd. Milk, blood, and fecal samples were collected, as well as tissue samples after the cows were culled. Serological analysis conducted on milk and serum samples resulted in positive reactions for MAP, but negative for Mycobacterium bovis. Production of IFN-gamma showed sensitization with mycobacteria or similar organisms, other than M. bovis, in one cow. Detection of MAP by bacteriological culture and IS900-based quantitative PCR on milk and feces remained negative. In conclusion, this paper describes M. smegmatis as a cause of bovine clinical mastitis in Belgium and suggests cross-reactivity of the intramammary M. smegmatis infection with routinely used serological tests for MAP.
Keywords
PARATUBERCULOSIS, IDENTIFICATION, bovine mastitis, Mycolicibacterium smegmatis, cross-reactivity, bovine, (para)tuberculosis

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MLA
Supré, K., et al. “Mycolicibacterium Smegmatis, Basonym Mycobacterium Smegmatis, Causing Pyogranulomatous Mastitis and Its Cross-Reactivity in Bovine (Para)Tuberculosis Testing.” JOURNAL OF DAIRY SCIENCE, vol. 102, no. 9, 2019, pp. 8405–09, doi:10.3168/jds.2019-16610.
APA
Supré, K., Roupie, V., Ribbens, S., Stevens, M., Boyen, F., & Roels, S. (2019). Mycolicibacterium smegmatis, basonym Mycobacterium smegmatis, causing pyogranulomatous mastitis and its cross-reactivity in bovine (para)tuberculosis testing. JOURNAL OF DAIRY SCIENCE, 102(9), 8405–8409. https://doi.org/10.3168/jds.2019-16610
Chicago author-date
Supré, K, V Roupie, S Ribbens, M Stevens, Filip Boyen, and S Roels. 2019. “Mycolicibacterium Smegmatis, Basonym Mycobacterium Smegmatis, Causing Pyogranulomatous Mastitis and Its Cross-Reactivity in Bovine (Para)Tuberculosis Testing.” JOURNAL OF DAIRY SCIENCE 102 (9): 8405–9. https://doi.org/10.3168/jds.2019-16610.
Chicago author-date (all authors)
Supré, K, V Roupie, S Ribbens, M Stevens, Filip Boyen, and S Roels. 2019. “Mycolicibacterium Smegmatis, Basonym Mycobacterium Smegmatis, Causing Pyogranulomatous Mastitis and Its Cross-Reactivity in Bovine (Para)Tuberculosis Testing.” JOURNAL OF DAIRY SCIENCE 102 (9): 8405–8409. doi:10.3168/jds.2019-16610.
Vancouver
1.
Supré K, Roupie V, Ribbens S, Stevens M, Boyen F, Roels S. Mycolicibacterium smegmatis, basonym Mycobacterium smegmatis, causing pyogranulomatous mastitis and its cross-reactivity in bovine (para)tuberculosis testing. JOURNAL OF DAIRY SCIENCE. 2019;102(9):8405–9.
IEEE
[1]
K. Supré, V. Roupie, S. Ribbens, M. Stevens, F. Boyen, and S. Roels, “Mycolicibacterium smegmatis, basonym Mycobacterium smegmatis, causing pyogranulomatous mastitis and its cross-reactivity in bovine (para)tuberculosis testing,” JOURNAL OF DAIRY SCIENCE, vol. 102, no. 9, pp. 8405–8409, 2019.
@article{8639550,
  abstract     = {Different mycobacterial species are encountered in bovine medicine. The fastidiously growing mycobacteria (Mycobacterium bovis as the cause of bovine tuberculosis, and Mycobacterium avium ssp. paratuberculosis, MAP, as the cause of paratuberculosis) are well known and targeted in eradication/control or monitoring programs in different countries, whereas the rapidly growing species is only rarely identified from bovine disease. The latter have occasionally been reported as the cause of bovine clinical mastitis, but recent reports are scarce. In this study, Mycolicibacterium smegmatis (basonym Mycobacterium smegmatis) was identified as cause of granulomatous, relapsing clinical mastitis in 2 cows from one Belgian dairy herd. Milk, blood, and fecal samples were collected, as well as tissue samples after the cows were culled. Serological analysis conducted on milk and serum samples resulted in positive reactions for MAP, but negative for Mycobacterium bovis. Production of IFN-gamma showed sensitization with mycobacteria or similar organisms, other than M. bovis, in one cow. Detection of MAP by bacteriological culture and IS900-based quantitative PCR on milk and feces remained negative. In conclusion, this paper describes M. smegmatis as a cause of bovine clinical mastitis in Belgium and suggests cross-reactivity of the intramammary M. smegmatis infection with routinely used serological tests for MAP.},
  author       = {Supré, K and Roupie, V and Ribbens, S and Stevens, M and Boyen, Filip and Roels, S},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keywords     = {PARATUBERCULOSIS,IDENTIFICATION,bovine mastitis,Mycolicibacterium smegmatis,cross-reactivity,bovine,(para)tuberculosis},
  language     = {eng},
  number       = {9},
  pages        = {8405--8409},
  title        = {Mycolicibacterium smegmatis, basonym Mycobacterium smegmatis, causing pyogranulomatous mastitis and its cross-reactivity in bovine (para)tuberculosis testing},
  url          = {http://dx.doi.org/10.3168/jds.2019-16610},
  volume       = {102},
  year         = {2019},
}

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