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Introgression of rol genes from rhizogenic Agrobacterium strains into Escallonia spp.

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Abstract
The introgression of rol-genes of rhizogenic Agrobacterium into the plant genome induces changes in plant phenotype and physiology. However, only limited experience with this technique is available for woody ornamentals. To induce new variation within the Escallonia genus, several species were co-cultivated with rhizogenic Agrobacterium strains. Co-cultivation of three rhizogenic Agrobacterium strains (Arqua1, LMG 63 and MAFF210266) with four Escallonia species (E. illinita, E. myrtoidea, E. rosea, and E. rubra), resulted in hairy roots production with a varying efficiency. Co-cultivation of E. rubra with MAFF210266, and E. myrtoidea with LMG63 did not yield hairy roots, while co-cultivation of E. rubra leaves with LMG63 was most successful for hairy root production (up to 80.6%). In addition, the efficiency of hairy root induction depended on the explant type (leaves or nodal sections). The presence of inserted rol-genes and aux-genes in hairy roots was molecularly confirmed using qPCR. Few shoots regenerated from hairy roots, but regeneration needs to be optimized for efficient implementation of rol-genes introgression in Escallonia breeding. Key Message: This research provides a protocol for the production of hairy roots with rol-genes inserted after co-cultivation of several species of Escallonia with rhizogenic Agrobacterium strains.
Keywords
Hairy roots, rol genes, Woody ornamental breeding, Agrobacterium rhizogenes, HAIRY-ROOT CULTURES, TRANSFORMED ROOTS, PCR PRIMERS, T-DNA, PLANTS, REGENERATION, L., PLASMID, EXPRESSION, INFECTION

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MLA
Denaeghel, Hanne, et al. “Introgression of Rol Genes from Rhizogenic Agrobacterium Strains into Escallonia Spp.” PLANT CELL TISSUE AND ORGAN CULTURE, vol. 140, no. 2, 2020, pp. 403–14, doi:10.1007/s11240-019-01736-x.
APA
Denaeghel, H., Desmet, S., De Keyser, E., Dhooghe, E., Eeckhaut, T., Van Huylenbroeck, J., … Van Laere, K. (2020). Introgression of rol genes from rhizogenic Agrobacterium strains into Escallonia spp. PLANT CELL TISSUE AND ORGAN CULTURE, 140(2), 403–414. https://doi.org/10.1007/s11240-019-01736-x
Chicago author-date
Denaeghel, Hanne, Siel Desmet, Ellen De Keyser, Emmy Dhooghe, Tom Eeckhaut, Johan Van Huylenbroeck, Marie-Christine Van Labeke, and Katrijn Van Laere. 2020. “Introgression of Rol Genes from Rhizogenic Agrobacterium Strains into Escallonia Spp.” PLANT CELL TISSUE AND ORGAN CULTURE 140 (2): 403–14. https://doi.org/10.1007/s11240-019-01736-x.
Chicago author-date (all authors)
Denaeghel, Hanne, Siel Desmet, Ellen De Keyser, Emmy Dhooghe, Tom Eeckhaut, Johan Van Huylenbroeck, Marie-Christine Van Labeke, and Katrijn Van Laere. 2020. “Introgression of Rol Genes from Rhizogenic Agrobacterium Strains into Escallonia Spp.” PLANT CELL TISSUE AND ORGAN CULTURE 140 (2): 403–414. doi:10.1007/s11240-019-01736-x.
Vancouver
1.
Denaeghel H, Desmet S, De Keyser E, Dhooghe E, Eeckhaut T, Van Huylenbroeck J, et al. Introgression of rol genes from rhizogenic Agrobacterium strains into Escallonia spp. PLANT CELL TISSUE AND ORGAN CULTURE. 2020;140(2):403–14.
IEEE
[1]
H. Denaeghel et al., “Introgression of rol genes from rhizogenic Agrobacterium strains into Escallonia spp.,” PLANT CELL TISSUE AND ORGAN CULTURE, vol. 140, no. 2, pp. 403–414, 2020.
@article{8639379,
  abstract     = {{The introgression of rol-genes of rhizogenic Agrobacterium into the plant genome induces changes in plant phenotype and physiology. However, only limited experience with this technique is available for woody ornamentals. To induce new variation within the Escallonia genus, several species were co-cultivated with rhizogenic Agrobacterium strains. Co-cultivation of three rhizogenic Agrobacterium strains (Arqua1, LMG 63 and MAFF210266) with four Escallonia species (E. illinita, E. myrtoidea, E. rosea, and E. rubra), resulted in hairy roots production with a varying efficiency. Co-cultivation of E. rubra with MAFF210266, and E. myrtoidea with LMG63 did not yield hairy roots, while co-cultivation of E. rubra leaves with LMG63 was most successful for hairy root production (up to 80.6%). In addition, the efficiency of hairy root induction depended on the explant type (leaves or nodal sections). The presence of inserted rol-genes and aux-genes in hairy roots was molecularly confirmed using qPCR. Few shoots regenerated from hairy roots, but regeneration needs to be optimized for efficient implementation of rol-genes introgression in Escallonia breeding. 
Key Message: This research provides a protocol for the production of hairy roots with rol-genes inserted after co-cultivation of several species of Escallonia with rhizogenic Agrobacterium strains.}},
  author       = {{Denaeghel, Hanne and Desmet, Siel and De Keyser, Ellen and Dhooghe, Emmy and Eeckhaut, Tom and Van Huylenbroeck, Johan and Van Labeke, Marie-Christine and Van Laere, Katrijn}},
  issn         = {{0167-6857}},
  journal      = {{PLANT CELL TISSUE AND ORGAN CULTURE}},
  keywords     = {{Hairy roots,rol genes,Woody ornamental breeding,Agrobacterium rhizogenes,HAIRY-ROOT CULTURES,TRANSFORMED ROOTS,PCR PRIMERS,T-DNA,PLANTS,REGENERATION,L.,PLASMID,EXPRESSION,INFECTION}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{403--414}},
  title        = {{Introgression of rol genes from rhizogenic Agrobacterium strains into Escallonia spp.}},
  url          = {{http://dx.doi.org/10.1007/s11240-019-01736-x}},
  volume       = {{140}},
  year         = {{2020}},
}

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