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CRISPR-Cas-mediated gene knockout in tomato

Gwen Swinnen (UGent) , Thomas Jacobs (UGent) , Laurens Pauwels (UGent) and Alain Goossens (UGent)
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Abstract
Loss-of-function mutants are crucial for plant functional genomics studies. With the advent of CRISPR-Cas genome editing, generating null alleles for one or multiple specific gene(s) has become feasible for many plant species including tomato (Solanum lycopersicum). An easily programmable RNA-guided Cas endonuclease efficiently creates DNA double-strand breaks (DSBs) at targeted genomic sites that can be repaired by nonhomologous end joining (NHEJ) typically leading to small insertions or deletions that can produce null mutations. Here, we describe how to utilize CRISPR-Cas genome editing to obtain stable tomato gene knockout lines.
Keywords
CRISRP-Cas, Gene knockout, Genome editing, Loss-of-function mutation, Null mutation, Site-directed mutagenesis, Solanaceae, Solanum lycopersicum, Targeted mutagenesis, Tomato

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MLA
Swinnen, Gwen, et al. “CRISPR-Cas-Mediated Gene Knockout in Tomato.” Plant and Food Carotenoids : Methods and Protocols, edited by Manuel Rodríguez-Concepción and Ralf Welsch, vol. 2083, Springer Humana Press, 2020, pp. 321–41.
APA
Swinnen, G., Jacobs, T., Pauwels, L., & Goossens, A. (2020). CRISPR-Cas-mediated gene knockout in tomato. In M. Rodríguez-Concepción & R. Welsch (Eds.), Plant and food carotenoids : methods and protocols (Vol. 2083, pp. 321–341). New York, NY, USA: Springer Humana Press.
Chicago author-date
Swinnen, Gwen, Thomas Jacobs, Laurens Pauwels, and Alain Goossens. 2020. “CRISPR-Cas-Mediated Gene Knockout in Tomato.” In Plant and Food Carotenoids : Methods and Protocols, edited by Manuel Rodríguez-Concepción and Ralf Welsch, 2083:321–41. New York, NY, USA: Springer Humana Press.
Chicago author-date (all authors)
Swinnen, Gwen, Thomas Jacobs, Laurens Pauwels, and Alain Goossens. 2020. “CRISPR-Cas-Mediated Gene Knockout in Tomato.” In Plant and Food Carotenoids : Methods and Protocols, ed by. Manuel Rodríguez-Concepción and Ralf Welsch, 2083:321–341. New York, NY, USA: Springer Humana Press.
Vancouver
1.
Swinnen G, Jacobs T, Pauwels L, Goossens A. CRISPR-Cas-mediated gene knockout in tomato. In: Rodríguez-Concepción M, Welsch R, editors. Plant and food carotenoids : methods and protocols. New York, NY, USA: Springer Humana Press; 2020. p. 321–41.
IEEE
[1]
G. Swinnen, T. Jacobs, L. Pauwels, and A. Goossens, “CRISPR-Cas-mediated gene knockout in tomato,” in Plant and food carotenoids : methods and protocols, vol. 2083, M. Rodríguez-Concepción and R. Welsch, Eds. New York, NY, USA: Springer Humana Press, 2020, pp. 321–341.
@incollection{8637718,
  abstract     = {Loss-of-function mutants are crucial for plant functional genomics studies. With the advent of CRISPR-Cas genome editing, generating null alleles for one or multiple specific gene(s) has become feasible for many plant species including tomato (Solanum lycopersicum). An easily programmable RNA-guided Cas endonuclease efficiently creates DNA double-strand breaks (DSBs) at targeted genomic sites that can be repaired by nonhomologous end joining (NHEJ) typically leading to small insertions or deletions that can produce null mutations. Here, we describe how to utilize CRISPR-Cas genome editing to obtain stable tomato gene knockout lines.},
  author       = {Swinnen, Gwen and Jacobs, Thomas and Pauwels, Laurens and Goossens, Alain},
  booktitle    = {Plant and food carotenoids : methods and protocols},
  editor       = {Rodríguez-Concepción, Manuel and Welsch, Ralf},
  isbn         = {9781493999514},
  issn         = {1064-3745},
  keywords     = {CRISRP-Cas,Gene knockout,Genome editing,Loss-of-function mutation,Null mutation,Site-directed mutagenesis,Solanaceae,Solanum lycopersicum,Targeted mutagenesis,Tomato},
  language     = {eng},
  pages        = {321--341},
  publisher    = {Springer Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {CRISPR-Cas-mediated gene knockout in tomato},
  url          = {http://dx.doi.org/10.1007/978-1-4939-9952-1_25},
  volume       = {2083},
  year         = {2020},
}

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