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Dual RNA sequencing of Vitis vinifera during Lasiodiplodia theobromae infection unveils host-pathogen interactions

Micael FM Gonçalves, Rui B Nunes, Laurentijn Tilleman (UGent) , Yves Van de Peer (UGent) , Dieter Deforce (UGent) , Filip Van Nieuwerburgh (UGent) , Ana C Esteves and Artur Alves
(2019) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 20(23).
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Abstract
Lasiodiplodia theobromae is one of the most aggressive agents of the grapevine trunk disease Botryosphaeria dieback. Through a dual RNA-sequencing approach, this study aimed to give a broader perspective on the infection strategy deployed by L. theobromae, while understanding grapevine response. Approximately 0.05% and 90% of the reads were mapped to the genomes of L. theobromae and Vitis vinifera, respectively. Over 2500 genes were significantly differentially expressed in infected plants after 10 dpi, many of which are involved in the inducible defense mechanisms of grapevines. Gene expression analysis showed changes in the fungal metabolism of phenolic compounds, carbohydrate metabolism, transmembrane transport, and toxin synthesis. These functions are related to the pathogenicity mechanisms involved in plant cell wall degradation and fungal defense against antimicrobial substances produced by the host. Genes encoding for the degradation of plant phenylpropanoid precursors were up-regulated, suggesting that the fungus could evade the host defense response using the phenylpropanoid pathway. The up-regulation of many distinct components of the phenylpropanoid pathway in plants supports this hypothesis. Moreover, genes related to phytoalexin biosynthesis, hormone metabolism, cell wall modification enzymes, and pathogenesis-related proteins seem to be involved in the host responses observed. This study provides additional insights into the molecular mechanisms of L. theobromae and V. vinifera interactions.
Keywords
dual RNA-Seq, grapevine, botryosphaeria dieback, plant defense, pathogenesis, BIOCONDUCTOR PACKAGE, EXPRESSION ANALYSIS, GRAPEVINE DECLINE, IDENTIFICATION, DEFENSE, BOTRYOSPHAERIACEAE, ARABIDOPSIS, THAUMATIN, PROTEINS, ENZYMES

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MLA
Gonçalves, Micael FM, et al. “Dual RNA Sequencing of Vitis Vinifera during Lasiodiplodia Theobromae Infection Unveils Host-Pathogen Interactions.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 23, 2019, doi:10.3390/ijms20236083.
APA
Gonçalves, M. F., Nunes, R. B., Tilleman, L., Van de Peer, Y., Deforce, D., Van Nieuwerburgh, F., … Alves, A. (2019). Dual RNA sequencing of Vitis vinifera during Lasiodiplodia theobromae infection unveils host-pathogen interactions. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(23). https://doi.org/10.3390/ijms20236083
Chicago author-date
Gonçalves, Micael FM, Rui B Nunes, Laurentijn Tilleman, Yves Van de Peer, Dieter Deforce, Filip Van Nieuwerburgh, Ana C Esteves, and Artur Alves. 2019. “Dual RNA Sequencing of Vitis Vinifera during Lasiodiplodia Theobromae Infection Unveils Host-Pathogen Interactions.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20 (23). https://doi.org/10.3390/ijms20236083.
Chicago author-date (all authors)
Gonçalves, Micael FM, Rui B Nunes, Laurentijn Tilleman, Yves Van de Peer, Dieter Deforce, Filip Van Nieuwerburgh, Ana C Esteves, and Artur Alves. 2019. “Dual RNA Sequencing of Vitis Vinifera during Lasiodiplodia Theobromae Infection Unveils Host-Pathogen Interactions.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20 (23). doi:10.3390/ijms20236083.
Vancouver
1.
Gonçalves MF, Nunes RB, Tilleman L, Van de Peer Y, Deforce D, Van Nieuwerburgh F, et al. Dual RNA sequencing of Vitis vinifera during Lasiodiplodia theobromae infection unveils host-pathogen interactions. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2019;20(23).
IEEE
[1]
M. F. Gonçalves et al., “Dual RNA sequencing of Vitis vinifera during Lasiodiplodia theobromae infection unveils host-pathogen interactions,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 23, 2019.
@article{8637716,
  abstract     = {{Lasiodiplodia theobromae is one of the most aggressive agents of the grapevine trunk disease Botryosphaeria dieback. Through a dual RNA-sequencing approach, this study aimed to give a broader perspective on the infection strategy deployed by L. theobromae, while understanding grapevine response. Approximately 0.05% and 90% of the reads were mapped to the genomes of L. theobromae and Vitis vinifera, respectively. Over 2500 genes were significantly differentially expressed in infected plants after 10 dpi, many of which are involved in the inducible defense mechanisms of grapevines. Gene expression analysis showed changes in the fungal metabolism of phenolic compounds, carbohydrate metabolism, transmembrane transport, and toxin synthesis. These functions are related to the pathogenicity mechanisms involved in plant cell wall degradation and fungal defense against antimicrobial substances produced by the host. Genes encoding for the degradation of plant phenylpropanoid precursors were up-regulated, suggesting that the fungus could evade the host defense response using the phenylpropanoid pathway. The up-regulation of many distinct components of the phenylpropanoid pathway in plants supports this hypothesis. Moreover, genes related to phytoalexin biosynthesis, hormone metabolism, cell wall modification enzymes, and pathogenesis-related proteins seem to be involved in the host responses observed. This study provides additional insights into the molecular mechanisms of L. theobromae and V. vinifera interactions.}},
  articleno    = {{6083}},
  author       = {{Gonçalves, Micael FM and Nunes, Rui B and Tilleman, Laurentijn and Van de Peer, Yves and Deforce, Dieter and Van Nieuwerburgh, Filip and Esteves, Ana C and Alves, Artur}},
  issn         = {{1422-0067}},
  journal      = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
  keywords     = {{dual RNA-Seq,grapevine,botryosphaeria dieback,plant defense,pathogenesis,BIOCONDUCTOR PACKAGE,EXPRESSION ANALYSIS,GRAPEVINE DECLINE,IDENTIFICATION,DEFENSE,BOTRYOSPHAERIACEAE,ARABIDOPSIS,THAUMATIN,PROTEINS,ENZYMES}},
  language     = {{eng}},
  number       = {{23}},
  pages        = {{19}},
  title        = {{Dual RNA sequencing of Vitis vinifera during Lasiodiplodia theobromae infection unveils host-pathogen interactions}},
  url          = {{http://doi.org/10.3390/ijms20236083}},
  volume       = {{20}},
  year         = {{2019}},
}
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