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Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery

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Abstract
The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these proteins are also involved in actin cytoskeleton regulated autophagy. Both AtEH/Pan1 proteins localise to the plasma membrane and autophagosomes. Upon induction of autophagy, AtEH/Pan1 proteins recruit TPC and AP-2 subunits, clathrin, actin and ARP2/3 proteins to autophagosomes. Increased expression of AtEH/Pan1 proteins boosts autophagosome formation, suggesting independent and redundant pathways for actin-mediated autophagy in plants. Moreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway is perturbed. In conclusion, we identify the existence of an autophagy-dependent pathway in plants to degrade endocytic components, starting at the EPCS through the interaction among AtEH/Pan1, actin cytoskeleton and the EPCS resident protein VAP27-1.
Keywords
PLASMA-MEMBRANE, ENDOPLASMIC-RETICULUM, PHOSPHATIDYLINOSITOL, 3-PHOSPHATE, COMPLEX, TPLATE, BIOGENESIS, ARP2/3, CYTOSKELETON, GENERATION, EXPRESSION

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MLA
Wang, Pengwei, et al. “Plant AtEH/Pan1 Proteins Drive Autophagosome Formation at ER-PM Contact Sites with Actin and Endocytic Machinery.” NATURE COMMUNICATIONS, vol. 10, 2019.
APA
Wang, P., Pleskot, R., Zang, J., Winkler, J., Wang, J., Yperman, K., … Hussey, P. J. (2019). Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery. NATURE COMMUNICATIONS, 10.
Chicago author-date
Wang, Pengwei, Roman Pleskot, Jingze Zang, Joanna Winkler, Jie Wang, Klaas Yperman, Tong Zhang, et al. 2019. “Plant AtEH/Pan1 Proteins Drive Autophagosome Formation at ER-PM Contact Sites with Actin and Endocytic Machinery.” NATURE COMMUNICATIONS 10.
Chicago author-date (all authors)
Wang, Pengwei, Roman Pleskot, Jingze Zang, Joanna Winkler, Jie Wang, Klaas Yperman, Tong Zhang, Kun Wang, Jinli Gong, Yajie Guan, Christine Richardson, Patrick Duckney, Michaël Vandorpe, Evelien Mylle, Jindriska Fiserova, Daniël Van Damme, and Patrick J Hussey. 2019. “Plant AtEH/Pan1 Proteins Drive Autophagosome Formation at ER-PM Contact Sites with Actin and Endocytic Machinery.” NATURE COMMUNICATIONS 10.
Vancouver
1.
Wang P, Pleskot R, Zang J, Winkler J, Wang J, Yperman K, et al. Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery. NATURE COMMUNICATIONS. 2019;10.
IEEE
[1]
P. Wang et al., “Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery,” NATURE COMMUNICATIONS, vol. 10, 2019.
@article{8637078,
  abstract     = {The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these proteins are also involved in actin cytoskeleton regulated autophagy. Both AtEH/Pan1 proteins localise to the plasma membrane and autophagosomes. Upon induction of autophagy, AtEH/Pan1 proteins recruit TPC and AP-2 subunits, clathrin, actin and ARP2/3 proteins to autophagosomes. Increased expression of AtEH/Pan1 proteins boosts autophagosome formation, suggesting independent and redundant pathways for actin-mediated autophagy in plants. Moreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway is perturbed. In conclusion, we identify the existence of an autophagy-dependent pathway in plants to degrade endocytic components, starting at the EPCS through the interaction among AtEH/Pan1, actin cytoskeleton and the EPCS resident protein VAP27-1.},
  articleno    = {5132},
  author       = {Wang, Pengwei and Pleskot, Roman and Zang, Jingze and Winkler, Joanna and Wang, Jie and Yperman, Klaas and Zhang, Tong and Wang, Kun and Gong, Jinli and Guan, Yajie and Richardson, Christine and Duckney, Patrick and Vandorpe, Michaël and Mylle, Evelien and Fiserova, Jindriska and Van Damme, Daniël and Hussey, Patrick J},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keywords     = {PLASMA-MEMBRANE,ENDOPLASMIC-RETICULUM,PHOSPHATIDYLINOSITOL,3-PHOSPHATE,COMPLEX,TPLATE,BIOGENESIS,ARP2/3,CYTOSKELETON,GENERATION,EXPRESSION},
  language     = {eng},
  pages        = {16},
  title        = {Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery},
  url          = {http://dx.doi.org/10.1038/s41467-019-12782-6},
  volume       = {10},
  year         = {2019},
}

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