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A metabolomics approach to unravel cricket paralysis virus infection in silkworm Bm5 cells

Luoluo Wang (UGent) , Luc Swevers, Caroline Rombouts (UGent) , Ivan Meeus (UGent) , Lieven Van Meulebroek (UGent) , Lynn Vanhaecke (UGent) and Guy Smagghe (UGent)
(2019) VIRUSES-BASEL. 11(9).
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Abstract
How a host metabolism responds to infection with insect viruses and how it relates to pathogenesis, is little investigated. Our previous study observed that Cricket paralysis virus (CrPV, Dicistroviridae) causes short term persistence in silkworm Bm5 cells before proceeding to acute infection. In this study, a metabolomics approach based on high resolution mass spectrometry was applied to investigate how a host metabolism is altered during the course of CrPV infection in Bm5 cells and which changes are characteristic for the transition from persistence to pathogenicity. We observed that CrPV infection led to significant and stage-specific metabolic changes in Bm5 cells. Differential metabolites abundance and pathway analysis further identified specific metabolic features at different stages in the viral life cycle. Notably, both glucose and glutamine levels significantly increased during CrPV persistent infection followed by a steep decrease during the pathogenic stages, suggesting that the central carbon metabolism was significantly modified during CrPV infection in Bm5 cells. In addition, dynamic changes in levels of polyamines were detected. Taken together, this study characterized for the first time the metabolic dynamics of CrPV infection in insect cells, proposing a central role for the regulation of both amino acid and carbohydrate metabolism during the period of persistent infection of CrPV in Bm5 cells.
Keywords
metabolomics, persistent infection, host metabolism, Cricket paralysis virus, silkworm, POLYAMINES, METABOLISM, STRATEGIES, ACID

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MLA
Wang, Luoluo, et al. “A Metabolomics Approach to Unravel Cricket Paralysis Virus Infection in Silkworm Bm5 Cells.” VIRUSES-BASEL, vol. 11, no. 9, 2019.
APA
Wang, L., Swevers, L., Rombouts, C., Meeus, I., Van Meulebroek, L., Vanhaecke, L., & Smagghe, G. (2019). A metabolomics approach to unravel cricket paralysis virus infection in silkworm Bm5 cells. VIRUSES-BASEL, 11(9).
Chicago author-date
Wang, Luoluo, Luc Swevers, Caroline Rombouts, Ivan Meeus, Lieven Van Meulebroek, Lynn Vanhaecke, and Guy Smagghe. 2019. “A Metabolomics Approach to Unravel Cricket Paralysis Virus Infection in Silkworm Bm5 Cells.” VIRUSES-BASEL 11 (9).
Chicago author-date (all authors)
Wang, Luoluo, Luc Swevers, Caroline Rombouts, Ivan Meeus, Lieven Van Meulebroek, Lynn Vanhaecke, and Guy Smagghe. 2019. “A Metabolomics Approach to Unravel Cricket Paralysis Virus Infection in Silkworm Bm5 Cells.” VIRUSES-BASEL 11 (9).
Vancouver
1.
Wang L, Swevers L, Rombouts C, Meeus I, Van Meulebroek L, Vanhaecke L, et al. A metabolomics approach to unravel cricket paralysis virus infection in silkworm Bm5 cells. VIRUSES-BASEL. 2019;11(9).
IEEE
[1]
L. Wang et al., “A metabolomics approach to unravel cricket paralysis virus infection in silkworm Bm5 cells,” VIRUSES-BASEL, vol. 11, no. 9, 2019.
@article{8635936,
  abstract     = {How a host metabolism responds to infection with insect viruses and how it relates to pathogenesis, is little investigated. Our previous study observed that Cricket paralysis virus (CrPV, Dicistroviridae) causes short term persistence in silkworm Bm5 cells before proceeding to acute infection. In this study, a metabolomics approach based on high resolution mass spectrometry was applied to investigate how a host metabolism is altered during the course of CrPV infection in Bm5 cells and which changes are characteristic for the transition from persistence to pathogenicity. We observed that CrPV infection led to significant and stage-specific metabolic changes in Bm5 cells. Differential metabolites abundance and pathway analysis further identified specific metabolic features at different stages in the viral life cycle. Notably, both glucose and glutamine levels significantly increased during CrPV persistent infection followed by a steep decrease during the pathogenic stages, suggesting that the central carbon metabolism was significantly modified during CrPV infection in Bm5 cells. In addition, dynamic changes in levels of polyamines were detected. Taken together, this study characterized for the first time the metabolic dynamics of CrPV infection in insect cells, proposing a central role for the regulation of both amino acid and carbohydrate metabolism during the period of persistent infection of CrPV in Bm5 cells.},
  articleno    = {861},
  author       = {Wang, Luoluo and Swevers, Luc and Rombouts, Caroline and Meeus, Ivan and Van Meulebroek, Lieven and Vanhaecke, Lynn and Smagghe, Guy},
  issn         = {1999-4915},
  journal      = {VIRUSES-BASEL},
  keywords     = {metabolomics,persistent infection,host metabolism,Cricket paralysis virus,silkworm,POLYAMINES,METABOLISM,STRATEGIES,ACID},
  language     = {eng},
  number       = {9},
  pages        = {18},
  title        = {A metabolomics approach to unravel cricket paralysis virus infection in silkworm Bm5 cells},
  url          = {http://dx.doi.org/10.3390/v11090861},
  volume       = {11},
  year         = {2019},
}

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