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Achromobacter veterisilvae sp. nov., from a mixed hydrogen-oxidizing bacteria enrichment reactor for microbial protein production

Charles Dumolin (UGent) , Charlotte Peeters (UGent) , Elham Ehsani (UGent) , Guillaume Tahon (UGent) , Evelien De Canck (UGent) , Margo Cnockaert (UGent) , Nico Boon (UGent) and Peter Vandamme (UGent)
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Abstract
Strain LMG 30378(T) was isolated from a hydrogen-oxidizing bacteria enrichment reactor inoculated with forest soil. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that this strain belonged to the genus Achromobacter. Multilocus sequence analysis combined with sequence analysis of a 765 bp nrdA gene fragment both showed Achromobacter agilis LMG 3411(T) and Achromobacter denitrificans LMG 1231(T) to be the closest-related neighbours to strain LMG 30378(T). Genome sequence analysis revealed a draft genome of 6.81 Mb with a G+C content of 67.2 mol%. In silico DNA-DNA hybridization with A. denitrificans LMG 1231(T) and A. agilis LMG 3411(T) showed 42.7 and 42.5% similarity, respectively, confirming that strain LMG 30378(T) represented a novel Achromobacter species. Phenotypic and metabolic characterization revealed acid phosphatase activity and the absence of phosphoamidase activity as distinctive features. The draft genome composes all necessary metabolic components to fix carbon dioxide and to oxidize molecular hydrogen, suggesting that strain LMG 30378(T) is a key organism in the enrichment reactor. Together, these data demonstrate that strain LMG 30378(T) represents a novel species of the genus Achromobacter, for which the name Achromobacter veterisilvae sp. nov. is proposed. The type strain is LMG 30378T (=CCUG 71558(T)).
Keywords
Achromobacter, hydrogen-oxidizing bacteria, HUMAN CLINICAL-SAMPLES, DENITRIFICANS RUGER, CLASSIFICATION, YABUUCHI, PROPOSAL

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MLA
Dumolin, Charles, et al. “Achromobacter Veterisilvae Sp. Nov., from a Mixed Hydrogen-Oxidizing Bacteria Enrichment Reactor for Microbial Protein Production.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 70, 2020, pp. 530–36.
APA
Dumolin, C., Peeters, C., Ehsani, E., Tahon, G., De Canck, E., Cnockaert, M., … Vandamme, P. (2020). Achromobacter veterisilvae sp. nov., from a mixed hydrogen-oxidizing bacteria enrichment reactor for microbial protein production. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 70, 530–536.
Chicago author-date
Dumolin, Charles, Charlotte Peeters, Elham Ehsani, Guillaume Tahon, Evelien De Canck, Margo Cnockaert, Nico Boon, and Peter Vandamme. 2020. “Achromobacter Veterisilvae Sp. Nov., from a Mixed Hydrogen-Oxidizing Bacteria Enrichment Reactor for Microbial Protein Production.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 70: 530–36.
Chicago author-date (all authors)
Dumolin, Charles, Charlotte Peeters, Elham Ehsani, Guillaume Tahon, Evelien De Canck, Margo Cnockaert, Nico Boon, and Peter Vandamme. 2020. “Achromobacter Veterisilvae Sp. Nov., from a Mixed Hydrogen-Oxidizing Bacteria Enrichment Reactor for Microbial Protein Production.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 70: 530–536.
Vancouver
1.
Dumolin C, Peeters C, Ehsani E, Tahon G, De Canck E, Cnockaert M, et al. Achromobacter veterisilvae sp. nov., from a mixed hydrogen-oxidizing bacteria enrichment reactor for microbial protein production. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2020;70:530–6.
IEEE
[1]
C. Dumolin et al., “Achromobacter veterisilvae sp. nov., from a mixed hydrogen-oxidizing bacteria enrichment reactor for microbial protein production,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 70, pp. 530–536, 2020.
@article{8635057,
  abstract     = {{Strain LMG 30378(T) was isolated from a hydrogen-oxidizing bacteria enrichment reactor inoculated with forest soil. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that this strain belonged to the genus Achromobacter. Multilocus sequence analysis combined with sequence analysis of a 765 bp nrdA gene fragment both showed Achromobacter agilis LMG 3411(T) and Achromobacter denitrificans LMG 1231(T) to be the closest-related neighbours to strain LMG 30378(T). Genome sequence analysis revealed a draft genome of 6.81 Mb with a G+C content of 67.2 mol%. In silico DNA-DNA hybridization with A. denitrificans LMG 1231(T) and A. agilis LMG 3411(T) showed 42.7 and 42.5% similarity, respectively, confirming that strain LMG 30378(T) represented a novel Achromobacter species. Phenotypic and metabolic characterization revealed acid phosphatase activity and the absence of phosphoamidase activity as distinctive features. The draft genome composes all necessary metabolic components to fix carbon dioxide and to oxidize molecular hydrogen, suggesting that strain LMG 30378(T) is a key organism in the enrichment reactor. Together, these data demonstrate that strain LMG 30378(T) represents a novel species of the genus Achromobacter, for which the name Achromobacter veterisilvae sp. nov. is proposed. The type strain is LMG 30378T (=CCUG 71558(T)).}},
  author       = {{Dumolin, Charles and Peeters, Charlotte and Ehsani, Elham and Tahon, Guillaume and De Canck, Evelien and Cnockaert, Margo and Boon, Nico and Vandamme, Peter}},
  issn         = {{1466-5026}},
  journal      = {{INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}},
  keywords     = {{Achromobacter,hydrogen-oxidizing bacteria,HUMAN CLINICAL-SAMPLES,DENITRIFICANS RUGER,CLASSIFICATION,YABUUCHI,PROPOSAL}},
  language     = {{eng}},
  pages        = {{530--536}},
  title        = {{Achromobacter veterisilvae sp. nov., from a mixed hydrogen-oxidizing bacteria enrichment reactor for microbial protein production}},
  url          = {{http://dx.doi.org/10.1099/ijsem.0.003786}},
  volume       = {{70}},
  year         = {{2020}},
}

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