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PIRIN2 suppresses S‐type lignin accumulation in a noncell‐autonomous manner in Arabidopsis xylem elements

Bo Zhang, Bernadette Sztojka, Sacha Escamez, Ruben Vanholme (UGent) , Mattias Hedenström, Yin Wang, Halbay Turumtay (UGent) , András Gorzsás, Wout Boerjan (UGent) and Hannele Tuominen
(2020) NEW PHYTOLOGIST. 225(5). p.1923-1935
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Abstract
PIRIN (PRN) genes encode cupin domain containing proteins that function as transcriptional co-regulators in humans but that are poorly described in plants. A previous study in xylogenic cell cultures of Zinnia elegans suggested a role for a PRN protein in lignification. This study aimed to identify function of Arabidopsis (Arabidopsis thaliana) PRN proteins in lignification of xylem tissues. Chemical composition of the secondary cell walls was analysed in Arabidopsis stems and/or hypocotyls by pyrolysis-gas chromatography/mass spectrometry, 2D-NMR and phenolic profiling. Secondary cell walls of individual xylem elements were chemotyped by Fourier Transform Infrared (FT-IR) and Raman microspectroscopy. Arabidopsis PRN2 suppressed accumulation of S-type lignin in Arabidopsis stems and hypocotyls. PRN2 promoter activity and PRN2:GFP fusion protein were localized specifically in cells next to the vessel elements, suggesting a role for PRN2 in non-cell-autonomous lignification of xylem vessels. Accordingly, PRN2 modulated lignin chemistry in the secondary cell walls of the neighboring vessel elements. These results indicate that PRN2 suppresses S-type lignin accumulation in the neighborhood of xylem vessels to bestow G-type enriched lignin composition on the secondary cell walls of the vessel elements. Gene expression analyses suggested that PRN2 function is mediated by regulation of the expression of the lignin-biosynthetic genes.
Keywords
Arabidopsis, lignification, noncell-autonomy, PIRIN, xylem vessel element, ORTHOGONAL PROJECTIONS, WALL DEPOSITION, LIGNIFICATION, BIOSYNTHESIS, EXPRESSION, CELLS, DIFFERENTIATION, IDENTIFICATION, HETEROGENEITY, RESPONSES, plant sciences

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MLA
Zhang, Bo, et al. “PIRIN2 Suppresses S‐type Lignin Accumulation in a Noncell‐autonomous Manner in Arabidopsis Xylem Elements.” NEW PHYTOLOGIST, vol. 225, no. 5, 2020, pp. 1923–35, doi:10.1111/nph.16271.
APA
Zhang, B., Sztojka, B., Escamez, S., Vanholme, R., Hedenström, M., Wang, Y., … Tuominen, H. (2020). PIRIN2 suppresses S‐type lignin accumulation in a noncell‐autonomous manner in Arabidopsis xylem elements. NEW PHYTOLOGIST, 225(5), 1923–1935. https://doi.org/10.1111/nph.16271
Chicago author-date
Zhang, Bo, Bernadette Sztojka, Sacha Escamez, Ruben Vanholme, Mattias Hedenström, Yin Wang, Halbay Turumtay, András Gorzsás, Wout Boerjan, and Hannele Tuominen. 2020. “PIRIN2 Suppresses S‐type Lignin Accumulation in a Noncell‐autonomous Manner in Arabidopsis Xylem Elements.” NEW PHYTOLOGIST 225 (5): 1923–35. https://doi.org/10.1111/nph.16271.
Chicago author-date (all authors)
Zhang, Bo, Bernadette Sztojka, Sacha Escamez, Ruben Vanholme, Mattias Hedenström, Yin Wang, Halbay Turumtay, András Gorzsás, Wout Boerjan, and Hannele Tuominen. 2020. “PIRIN2 Suppresses S‐type Lignin Accumulation in a Noncell‐autonomous Manner in Arabidopsis Xylem Elements.” NEW PHYTOLOGIST 225 (5): 1923–1935. doi:10.1111/nph.16271.
Vancouver
1.
Zhang B, Sztojka B, Escamez S, Vanholme R, Hedenström M, Wang Y, et al. PIRIN2 suppresses S‐type lignin accumulation in a noncell‐autonomous manner in Arabidopsis xylem elements. NEW PHYTOLOGIST. 2020;225(5):1923–35.
IEEE
[1]
B. Zhang et al., “PIRIN2 suppresses S‐type lignin accumulation in a noncell‐autonomous manner in Arabidopsis xylem elements,” NEW PHYTOLOGIST, vol. 225, no. 5, pp. 1923–1935, 2020.
@article{8632663,
  abstract     = {{PIRIN (PRN) genes encode cupin domain containing proteins that function as transcriptional co-regulators in humans but that are poorly described in plants. A previous study in xylogenic cell cultures of Zinnia elegans suggested a role for a PRN protein in lignification. This study aimed to identify function of Arabidopsis (Arabidopsis thaliana) PRN proteins in lignification of xylem tissues. Chemical composition of the secondary cell walls was analysed in Arabidopsis stems and/or hypocotyls by pyrolysis-gas chromatography/mass spectrometry, 2D-NMR and phenolic profiling. Secondary cell walls of individual xylem elements were chemotyped by Fourier Transform Infrared (FT-IR) and Raman microspectroscopy. Arabidopsis PRN2 suppressed accumulation of S-type lignin in Arabidopsis stems and hypocotyls. PRN2 promoter activity and PRN2:GFP fusion protein were localized specifically in cells next to the vessel elements, suggesting a role for PRN2 in non-cell-autonomous lignification of xylem vessels. Accordingly, PRN2 modulated lignin chemistry in the secondary cell walls of the neighboring vessel elements. These results indicate that PRN2 suppresses S-type lignin accumulation in the neighborhood of xylem vessels to bestow G-type enriched lignin composition on the secondary cell walls of the vessel elements. Gene expression analyses suggested that PRN2 function is mediated by regulation of the expression of the lignin-biosynthetic genes.}},
  author       = {{Zhang, Bo and Sztojka, Bernadette and Escamez, Sacha and Vanholme, Ruben and Hedenström, Mattias and Wang, Yin and Turumtay, Halbay and Gorzsás, András and Boerjan, Wout and Tuominen, Hannele}},
  issn         = {{0028-646X}},
  journal      = {{NEW PHYTOLOGIST}},
  keywords     = {{Arabidopsis,lignification,noncell-autonomy,PIRIN,xylem vessel element,ORTHOGONAL PROJECTIONS,WALL DEPOSITION,LIGNIFICATION,BIOSYNTHESIS,EXPRESSION,CELLS,DIFFERENTIATION,IDENTIFICATION,HETEROGENEITY,RESPONSES,plant sciences}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1923--1935}},
  title        = {{PIRIN2 suppresses S‐type lignin accumulation in a noncell‐autonomous manner in Arabidopsis xylem elements}},
  url          = {{http://doi.org/10.1111/nph.16271}},
  volume       = {{225}},
  year         = {{2020}},
}
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