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Texture profile analysis reveals a stiffer ovarian cortex after testosterone therapy : a pilot study

Chloë De Roo (UGent) , Kelly Tilleman (UGent) , Chris Vercruysse (UGent) , Heidi Declercq (UGent) , Guy T'Sjoen (UGent) , Steven Weyers (UGent) and Petra De Sutter (UGent)
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Abstract
Purpose: The importance of the surrounding ovarian stromal cells and extracellular matrix in the development and maturation of follicles has recently gained attention. An aberrant extracellular matrix has been described in ovaries of patients with polycystic ovary syndrome where a more rigid structural environment, possibly induced by endogenous testosterone, impairs normal folliculogenesis. In this context, we describe the textural parameters of the ovarian cortex of transgender men after prolonged testosterone administration compared to the textural parameters of the non-exposed ovarian cortex originating from female oncological patients. Methods: Texture profile analysis (TPA) was performed on ovarian cortex (5 x 5 mm) of oncological and transgender patients in order to measure stiffness, hardness, cohesiveness, and springiness of the ovarian cortex (LRXplus universal testing system). Statistical analysis was performed using repeated measurements mixed models and the Spearman rank order correlation test (IBM SPSS Statistics 23). Results: A total of 36 frozen-thawed cortical strips (5 x 5 mm) were subjected to TPA. The superficial part of cortex fragments originating from transgender persons (fragments < 1.4 mm; N = 10) appeared to be significantly stiffer compared to cortex derived from oncology patients (fragments < 1.4 mm; N = 7) (6.78 +/- 1.38 N/mm versus 5.41 +/- 0.9 N/mm respectively, p = 0.036). Conclusions: This is the first application of TPA in ovarian cortex to study the physical properties. Comparing the physical properties, we objectively describe an increased cortical stiffness in the most outer part of the ovarian cortex following prolonged testosterone administration in transgender men compared to the ovarian cortex of oncological patients. This preliminary and novel approach could be the start of future research to understand the physical properties of ovarian tissue.
Keywords
FOLLICLE DEVELOPMENT, ANDROGEN RECEPTOR, TISSUE, CULTURE, RIGIDITY, Cortex, Ovary, Stroma, Testosterone, Transgender, Texture profile, analysis

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MLA
De Roo, Chloë, et al. “Texture Profile Analysis Reveals a Stiffer Ovarian Cortex after Testosterone Therapy : A Pilot Study.” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 36, no. 9, 2019, pp. 1837–43.
APA
De Roo, C., Tilleman, K., Vercruysse, C., Declercq, H., T’Sjoen, G., Weyers, S., & De Sutter, P. (2019). Texture profile analysis reveals a stiffer ovarian cortex after testosterone therapy : a pilot study. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 36(9), 1837–1843.
Chicago author-date
De Roo, Chloë, Kelly Tilleman, Chris Vercruysse, Heidi Declercq, Guy T’Sjoen, Steven Weyers, and Petra De Sutter. 2019. “Texture Profile Analysis Reveals a Stiffer Ovarian Cortex after Testosterone Therapy : A Pilot Study.” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 36 (9): 1837–43.
Chicago author-date (all authors)
De Roo, Chloë, Kelly Tilleman, Chris Vercruysse, Heidi Declercq, Guy T’Sjoen, Steven Weyers, and Petra De Sutter. 2019. “Texture Profile Analysis Reveals a Stiffer Ovarian Cortex after Testosterone Therapy : A Pilot Study.” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 36 (9): 1837–1843.
Vancouver
1.
De Roo C, Tilleman K, Vercruysse C, Declercq H, T’Sjoen G, Weyers S, et al. Texture profile analysis reveals a stiffer ovarian cortex after testosterone therapy : a pilot study. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS. 2019;36(9):1837–43.
IEEE
[1]
C. De Roo et al., “Texture profile analysis reveals a stiffer ovarian cortex after testosterone therapy : a pilot study,” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 36, no. 9, pp. 1837–1843, 2019.
@article{8632607,
  abstract     = {Purpose: The importance of the surrounding ovarian stromal cells and extracellular matrix in the development and maturation of follicles has recently gained attention. An aberrant extracellular matrix has been described in ovaries of patients with polycystic ovary syndrome where a more rigid structural environment, possibly induced by endogenous testosterone, impairs normal folliculogenesis. In this context, we describe the textural parameters of the ovarian cortex of transgender men after prolonged testosterone administration compared to the textural parameters of the non-exposed ovarian cortex originating from female oncological patients.
Methods: Texture profile analysis (TPA) was performed on ovarian cortex (5 x 5 mm) of oncological and transgender patients in order to measure stiffness, hardness, cohesiveness, and springiness of the ovarian cortex (LRXplus universal testing system). Statistical analysis was performed using repeated measurements mixed models and the Spearman rank order correlation test (IBM SPSS Statistics 23).
Results: A total of 36 frozen-thawed cortical strips (5 x 5 mm) were subjected to TPA. The superficial part of cortex fragments originating from transgender persons (fragments < 1.4 mm; N = 10) appeared to be significantly stiffer compared to cortex derived from oncology patients (fragments < 1.4 mm; N = 7) (6.78 +/- 1.38 N/mm versus 5.41 +/- 0.9 N/mm respectively, p = 0.036).
Conclusions: This is the first application of TPA in ovarian cortex to study the physical properties. Comparing the physical properties, we objectively describe an increased cortical stiffness in the most outer part of the ovarian cortex following prolonged testosterone administration in transgender men compared to the ovarian cortex of oncological patients. This preliminary and novel approach could be the start of future research to understand the physical properties of ovarian tissue.},
  author       = {De Roo, Chloë and Tilleman, Kelly and Vercruysse, Chris and Declercq, Heidi and T'Sjoen, Guy and Weyers, Steven and De Sutter, Petra},
  issn         = {1058-0468},
  journal      = {JOURNAL OF ASSISTED REPRODUCTION AND GENETICS},
  keywords     = {FOLLICLE DEVELOPMENT,ANDROGEN RECEPTOR,TISSUE,CULTURE,RIGIDITY,Cortex,Ovary,Stroma,Testosterone,Transgender,Texture profile,analysis},
  language     = {eng},
  number       = {9},
  pages        = {1837--1843},
  title        = {Texture profile analysis reveals a stiffer ovarian cortex after testosterone therapy : a pilot study},
  url          = {http://dx.doi.org/10.1007/s10815-019-01513-x},
  volume       = {36},
  year         = {2019},
}

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