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Biocompatible lipid‐coated persistent luminescent nanoparticles for in vivo imaging of dendritic cell migration

Aranit Harizaj (UGent) , Olivier De Clercq (UGent) , Benedicte Descamps (UGent) , Christian Vanhove (UGent) , Stefaan De Smedt (UGent) , Dirk Poelman (UGent) , Ine Lentacker (UGent) and Kevin Braeckmans (UGent)
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Abstract
Dendritic cell (DC)-based vaccines for immunotherapy have already achieved promising results in the last decade. To further improve current treatment protocols and enhance the therapeutic outcome, noninvasive in vivo tracking of DCs remains of crucial importance. Persistent luminescent nanoparticles (PLNPs) are inorganic materials which show an afterglow for hours after the optical excitation has ceased. If the afterglow is in the near-infrared, the emission of injected particles can be tracked in vivo. However, stability and toxicity issues limit the use of bare PLNPs for biological applications. Therefore, appropriate surface functionalization is needed to improve their biocompatibility. In this study, it is demonstrated that near-infrared light emitting LiGa5O8:Cr3+ nanoparticles can be functionalized with a biocompatible lipid coating which provides them with outstanding stability in biological media. In vitro experiments show efficient uptake, absence of cytotoxicity even at very high particle concentrations, and no adverse effects on the maturation potential of DCs. DCs labeled with lipid-coated LiGa5O8:Cr3+ nanoparticles injected in mice can be imaged over days, confirming efficient in vivo migration to the popliteal lymph node. Together the results show that lipid coated LiGa5O8:Cr3+ nanoparticles possess excellent possibilities for further use in research and development of DC based vaccines.
Keywords
bioimaging, dendritic cells, immunotherapy, lipid coatings, persistent luminescence nanoparticles, MELANOMA PATIENTS, CANCER-CELLS, LYMPH-NODES, MRI PROBES, TRACKING, STIMULATION, NANOPROBES, MATURATION, STABILITY, DELIVERY

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MLA
Harizaj, Aranit, et al. “Biocompatible Lipid‐coated Persistent Luminescent Nanoparticles for in Vivo Imaging of Dendritic Cell Migration.” PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION, edited by Aranit Harizaj, 2019.
APA
Harizaj, A., De Clercq, O., Descamps, B., Vanhove, C., De Smedt, S., Poelman, D., … Braeckmans, K. (2019). Biocompatible lipid‐coated persistent luminescent nanoparticles for in vivo imaging of dendritic cell migration. PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION.
Chicago author-date
Harizaj, Aranit, Olivier De Clercq, Benedicte Descamps, Christian Vanhove, Stefaan De Smedt, Dirk Poelman, Ine Lentacker, and Kevin Braeckmans. 2019. “Biocompatible Lipid‐coated Persistent Luminescent Nanoparticles for in Vivo Imaging of Dendritic Cell Migration.” Edited by Aranit Harizaj. PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION.
Chicago author-date (all authors)
Harizaj, Aranit, Olivier De Clercq, Benedicte Descamps, Christian Vanhove, Stefaan De Smedt, Dirk Poelman, Ine Lentacker, and Kevin Braeckmans. 2019. “Biocompatible Lipid‐coated Persistent Luminescent Nanoparticles for in Vivo Imaging of Dendritic Cell Migration.” Ed by. Aranit Harizaj. PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION.
Vancouver
1.
Harizaj A, De Clercq O, Descamps B, Vanhove C, De Smedt S, Poelman D, et al. Biocompatible lipid‐coated persistent luminescent nanoparticles for in vivo imaging of dendritic cell migration. Harizaj A, editor. PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION. 2019;
IEEE
[1]
A. Harizaj et al., “Biocompatible lipid‐coated persistent luminescent nanoparticles for in vivo imaging of dendritic cell migration,” PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION, 2019.
@article{8632175,
  abstract     = {Dendritic cell (DC)-based vaccines for immunotherapy have already achieved promising results in the last decade. To further improve current treatment protocols and enhance the therapeutic outcome, noninvasive in vivo tracking of DCs remains of crucial importance. Persistent luminescent nanoparticles (PLNPs) are inorganic materials which show an afterglow for hours after the optical excitation has ceased. If the afterglow is in the near-infrared, the emission of injected particles can be tracked in vivo. However, stability and toxicity issues limit the use of bare PLNPs for biological applications. Therefore, appropriate surface functionalization is needed to improve their biocompatibility. In this study, it is demonstrated that near-infrared light emitting LiGa5O8:Cr3+ nanoparticles can be functionalized with a biocompatible lipid coating which provides them with outstanding stability in biological media. In vitro experiments show efficient uptake, absence of cytotoxicity even at very high particle concentrations, and no adverse effects on the maturation potential of DCs. DCs labeled with lipid-coated LiGa5O8:Cr3+ nanoparticles injected in mice can be imaged over days, confirming efficient in vivo migration to the popliteal lymph node. Together the results show that lipid coated LiGa5O8:Cr3+ nanoparticles possess excellent possibilities for further use in research and development of DC based vaccines.},
  articleno    = {1900371},
  author       = {Harizaj, Aranit and De Clercq, Olivier and Descamps, Benedicte and Vanhove, Christian and De Smedt, Stefaan and Poelman, Dirk and Lentacker, Ine and Braeckmans, Kevin},
  editor       = {Harizaj, Aranit},
  issn         = {0934-0866},
  journal      = {PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION},
  keywords     = {bioimaging,dendritic cells,immunotherapy,lipid coatings,persistent luminescence nanoparticles,MELANOMA PATIENTS,CANCER-CELLS,LYMPH-NODES,MRI PROBES,TRACKING,STIMULATION,NANOPROBES,MATURATION,STABILITY,DELIVERY},
  language     = {eng},
  title        = {Biocompatible lipid‐coated persistent luminescent nanoparticles for in vivo imaging of dendritic cell migration},
  url          = {http://dx.doi.org/10.1002/ppsc.201900371},
  year         = {2019},
}

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