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Production of long-chain hydroxy fatty acids by Starmerella bombicola

Marilyn De Graeve (UGent) , Isabelle Van de Velde (UGent) , Lien Saey (UGent) , Maarten Chys, Hanne Oorts (UGent) , Hümeyra Kahriman, Stein Mincke (UGent) , Christian Stevens (UGent) , Sofie De Maeseneire (UGent) , Sophie Roelants (UGent) , et al.
(2019) FEMS YEAST RESEARCH. 19(7).
Author
Organization
Abstract
To decrease our dependency for the diminishing source of fossils resources, bio-based alternatives are being explored for the synthesis of commodity and high-value molecules. One example in this ecological initiative is the microbial production of the biosurfactant sophorolipids by the yeast Starmerella bombicola. Sophorolipids are surface-active molecules mainly used as household and laundry detergents. Because S. bombicola is able to produce high titers of sophorolipids, the yeast is also used to increase the portfolio of lipophilic compounds through strain engineering. Here, the one-step microbial production of hydroxy fatty acids by S. bombicola was accomplished by the selective blockage of three catabolic pathways through metabolic engineering. Successful production of 17.39 g/l (omega-1) linked hydroxy fatty acids was obtained by the successive blockage of the sophorolipid biosynthesis, the beta-oxidation and the omega-oxidation pathways. Minor contamination of dicarboxylic acids and fatty aldehydes were successfully removed using flash chromatography. This way, S. bombicola was further expanded into a flexible production platform of economical relevant compounds in the chemical, food and cosmetic industries.
Keywords
Starmerella bombicola, sophorolipid, beta-oxidation, omega-oxidation, hydroxy fatty acid, dicarboxylic acid, ACYL-COA OXIDASE, CANDIDA-TROPICALIS, TORULOPSIS-BOMBICOLA, OMEGA-HYDROXYLATION, BETA-OXIDATION, GENE, SOPHOROLIPIDS, CYTOCHROME-P450, IDENTIFICATION, STEP

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Citation

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MLA
De Graeve, Marilyn, et al. “Production of Long-Chain Hydroxy Fatty Acids by Starmerella Bombicola.” FEMS YEAST RESEARCH, vol. 19, no. 7, 2019, doi:10.1093/femsyr/foz067.
APA
De Graeve, M., Van de Velde, I., Saey, L., Chys, M., Oorts, H., Kahriman, H., … Soetaert, W. (2019). Production of long-chain hydroxy fatty acids by Starmerella bombicola. FEMS YEAST RESEARCH, 19(7). https://doi.org/10.1093/femsyr/foz067
Chicago author-date
De Graeve, Marilyn, Isabelle Van de Velde, Lien Saey, Maarten Chys, Hanne Oorts, Hümeyra Kahriman, Stein Mincke, et al. 2019. “Production of Long-Chain Hydroxy Fatty Acids by Starmerella Bombicola.” FEMS YEAST RESEARCH 19 (7). https://doi.org/10.1093/femsyr/foz067.
Chicago author-date (all authors)
De Graeve, Marilyn, Isabelle Van de Velde, Lien Saey, Maarten Chys, Hanne Oorts, Hümeyra Kahriman, Stein Mincke, Christian Stevens, Sofie De Maeseneire, Sophie Roelants, and Wim Soetaert. 2019. “Production of Long-Chain Hydroxy Fatty Acids by Starmerella Bombicola.” FEMS YEAST RESEARCH 19 (7). doi:10.1093/femsyr/foz067.
Vancouver
1.
De Graeve M, Van de Velde I, Saey L, Chys M, Oorts H, Kahriman H, et al. Production of long-chain hydroxy fatty acids by Starmerella bombicola. FEMS YEAST RESEARCH. 2019;19(7).
IEEE
[1]
M. De Graeve et al., “Production of long-chain hydroxy fatty acids by Starmerella bombicola,” FEMS YEAST RESEARCH, vol. 19, no. 7, 2019.
@article{8632016,
  abstract     = {{To decrease our dependency for the diminishing source of fossils resources, bio-based alternatives are being explored for the synthesis of commodity and high-value molecules. One example in this ecological initiative is the microbial production of the biosurfactant sophorolipids by the yeast Starmerella bombicola. Sophorolipids are surface-active molecules mainly used as household and laundry detergents. Because S. bombicola is able to produce high titers of sophorolipids, the yeast is also used to increase the portfolio of lipophilic compounds through strain engineering. Here, the one-step microbial production of hydroxy fatty acids by S. bombicola was accomplished by the selective blockage of three catabolic pathways through metabolic engineering. Successful production of 17.39 g/l (omega-1) linked hydroxy fatty acids was obtained by the successive blockage of the sophorolipid biosynthesis, the beta-oxidation and the omega-oxidation pathways. Minor contamination of dicarboxylic acids and fatty aldehydes were successfully removed using flash chromatography. This way, S. bombicola was further expanded into a flexible production platform of economical relevant compounds in the chemical, food and cosmetic industries.}},
  articleno    = {{foz067}},
  author       = {{De Graeve, Marilyn and Van de Velde, Isabelle and Saey, Lien and Chys, Maarten and Oorts, Hanne and Kahriman, Hümeyra and Mincke, Stein and Stevens, Christian and De Maeseneire, Sofie and Roelants, Sophie and Soetaert, Wim}},
  issn         = {{1567-1356}},
  journal      = {{FEMS YEAST RESEARCH}},
  keywords     = {{Starmerella bombicola,sophorolipid,beta-oxidation,omega-oxidation,hydroxy fatty acid,dicarboxylic acid,ACYL-COA OXIDASE,CANDIDA-TROPICALIS,TORULOPSIS-BOMBICOLA,OMEGA-HYDROXYLATION,BETA-OXIDATION,GENE,SOPHOROLIPIDS,CYTOCHROME-P450,IDENTIFICATION,STEP}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{9}},
  title        = {{Production of long-chain hydroxy fatty acids by Starmerella bombicola}},
  url          = {{http://doi.org/10.1093/femsyr/foz067}},
  volume       = {{19}},
  year         = {{2019}},
}
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