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Non-aureus staphylococci in fecal samples of dairy cows : first report and phenotypic and genotypic characterization

Ameline Wuytack (UGent) , Anneleen De Visscher (UGent) , Sofie Piepers (UGent) , Filip Boyen (UGent) , Freddy Haesebrouck (UGent) and Sarne De Vliegher (UGent)
(2019) JOURNAL OF DAIRY SCIENCE. 102(10). p.9345-9359
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Abstract
The aims of this study were to determine whether non-aureus staphylococci (NAS) are present in rectal feces of healthy dairy cows, and if so, to delineate species to which they belong and to study several phenotypic and genotypic traits as a first step toward determining the potential impact of fecal shedding of NAS on bovine udder health. Fecal samples were aseptically collected from the rectum of 25 randomly selected clinically healthy dairy cows in a commercial dairy herd using an automated milking system. Fecal NAS were isolated and then identified at the species level using transfer RNA-intergenic spacer PCR and sequencing of the 16S rRNA housekeeping gene. Strain typing was performed using random amplification of polymorphic DNA (RAPD)-PCR. The antimicrobial resistance profiles, biofilm formation, and growth and inhibitory characteristics of all NAS isolates were evaluated. Half of the cows were shedding NAS, resulting in 31 NAS isolates belonging to 11 different species. The most prevalent species were Staphylococcus rostri (23%, n = 7), Staphylococcus cohnii (16%, n = 5), and Staphylococcus haemolyticus (13%, n = 4) with all Staphylococcus agnetis, Staphylococcus chromogenes, and Staph. rostri isolates belonging to the same strain according to RAPD banding patterns. Acquired antimicrobial resistance was observed in 28 of the 31 NAS isolates, mainly due to beta-lactamase production. Most of the isolates (84%, n = 27) had a weak biofilrn-forming potential, but only 2 contained the bap gene. The ica and aap genes were not detected in any of the isolates. In vitro growth of Staphylococcus aureus and Streptococcus dysgalactiae was inhibited by Staph. agnetis isolates, and Staph. chromogenes isolates were able to inhibit the growth of Strep. dysgalactiae and Streptococcus uberis. All fecal isolates were able to grow when oxygen and iron were limitedly available, mimicking the growth conditions in the mammary gland.
Keywords
dairy cow, feces, mastitis, non-aureus staphylococci, COAGULASE-NEGATIVE STAPHYLOCOCCI, SOMATIC-CELL COUNT, BOVINE MASTITIS, ANTIMICROBIAL RESISTANCE, INTRAMAMMARY INFECTION, METHICILLIN RESISTANCE, ANTIBIOTIC-RESISTANCE, KLEBSIELLA-PNEUMONIAE, BIOFILM FORMATION, ESCHERICHIA-COLI

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Citation

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MLA
Wuytack, Ameline, et al. “Non-Aureus Staphylococci in Fecal Samples of Dairy Cows : First Report and Phenotypic and Genotypic Characterization.” JOURNAL OF DAIRY SCIENCE, vol. 102, no. 10, 2019, pp. 9345–59.
APA
Wuytack, A., De Visscher, A., Piepers, S., Boyen, F., Haesebrouck, F., & De Vliegher, S. (2019). Non-aureus staphylococci in fecal samples of dairy cows : first report and phenotypic and genotypic characterization. JOURNAL OF DAIRY SCIENCE, 102(10), 9345–9359.
Chicago author-date
Wuytack, Ameline, Anneleen De Visscher, Sofie Piepers, Filip Boyen, Freddy Haesebrouck, and Sarne De Vliegher. 2019. “Non-Aureus Staphylococci in Fecal Samples of Dairy Cows : First Report and Phenotypic and Genotypic Characterization.” JOURNAL OF DAIRY SCIENCE 102 (10): 9345–59.
Chicago author-date (all authors)
Wuytack, Ameline, Anneleen De Visscher, Sofie Piepers, Filip Boyen, Freddy Haesebrouck, and Sarne De Vliegher. 2019. “Non-Aureus Staphylococci in Fecal Samples of Dairy Cows : First Report and Phenotypic and Genotypic Characterization.” JOURNAL OF DAIRY SCIENCE 102 (10): 9345–9359.
Vancouver
1.
Wuytack A, De Visscher A, Piepers S, Boyen F, Haesebrouck F, De Vliegher S. Non-aureus staphylococci in fecal samples of dairy cows : first report and phenotypic and genotypic characterization. JOURNAL OF DAIRY SCIENCE. 2019;102(10):9345–59.
IEEE
[1]
A. Wuytack, A. De Visscher, S. Piepers, F. Boyen, F. Haesebrouck, and S. De Vliegher, “Non-aureus staphylococci in fecal samples of dairy cows : first report and phenotypic and genotypic characterization,” JOURNAL OF DAIRY SCIENCE, vol. 102, no. 10, pp. 9345–9359, 2019.
@article{8631986,
  abstract     = {{The aims of this study were to determine whether non-aureus staphylococci (NAS) are present in rectal feces of healthy dairy cows, and if so, to delineate species to which they belong and to study several phenotypic and genotypic traits as a first step toward determining the potential impact of fecal shedding of NAS on bovine udder health. Fecal samples were aseptically collected from the rectum of 25 randomly selected clinically healthy dairy cows in a commercial dairy herd using an automated milking system. Fecal NAS were isolated and then identified at the species level using transfer RNA-intergenic spacer PCR and sequencing of the 16S rRNA housekeeping gene. Strain typing was performed using random amplification of polymorphic DNA (RAPD)-PCR. The antimicrobial resistance profiles, biofilm formation, and growth and inhibitory characteristics of all NAS isolates were evaluated. Half of the cows were shedding NAS, resulting in 31 NAS isolates belonging to 11 different species. The most prevalent species were Staphylococcus rostri (23%, n = 7), Staphylococcus cohnii (16%, n = 5), and Staphylococcus haemolyticus (13%, n = 4) with all Staphylococcus agnetis, Staphylococcus chromogenes, and Staph. rostri isolates belonging to the same strain according to RAPD banding patterns. Acquired antimicrobial resistance was observed in 28 of the 31 NAS isolates, mainly due to beta-lactamase production. Most of the isolates (84%, n = 27) had a weak biofilrn-forming potential, but only 2 contained the bap gene. The ica and aap genes were not detected in any of the isolates. In vitro growth of Staphylococcus aureus and Streptococcus dysgalactiae was inhibited by Staph. agnetis isolates, and Staph. chromogenes isolates were able to inhibit the growth of Strep. dysgalactiae and Streptococcus uberis. All fecal isolates were able to grow when oxygen and iron were limitedly available, mimicking the growth conditions in the mammary gland.}},
  author       = {{Wuytack, Ameline and De Visscher, Anneleen and Piepers, Sofie and Boyen, Filip and Haesebrouck, Freddy and De Vliegher, Sarne}},
  issn         = {{0022-0302}},
  journal      = {{JOURNAL OF DAIRY SCIENCE}},
  keywords     = {{dairy cow,feces,mastitis,non-aureus staphylococci,COAGULASE-NEGATIVE STAPHYLOCOCCI,SOMATIC-CELL COUNT,BOVINE MASTITIS,ANTIMICROBIAL RESISTANCE,INTRAMAMMARY INFECTION,METHICILLIN RESISTANCE,ANTIBIOTIC-RESISTANCE,KLEBSIELLA-PNEUMONIAE,BIOFILM FORMATION,ESCHERICHIA-COLI}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{9345--9359}},
  title        = {{Non-aureus staphylococci in fecal samples of dairy cows : first report and phenotypic and genotypic characterization}},
  url          = {{http://dx.doi.org/10.3168/jds.2019-16662}},
  volume       = {{102}},
  year         = {{2019}},
}

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