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A guide to Mycobacterium mutagenesis

Katlyn Borgers (UGent) , Kristof Vandewalle (UGent) , Nele Festjens (UGent) and Nico Callewaert (UGent)
(2019) FEBS JOURNAL. 286(19). p.3757-3774
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Abstract
The genus Mycobacterium includes several pathogens that cause severe disease in humans, like Mycobacterium tuberculosis (M. tb), the infectious agent causing tuberculosis. Genetic tools to engineer mycobacterial genomes, in a targeted or random fashion, have provided opportunities to investigate M. tb infection and pathogenesis. Furthermore, they have allowed the identification and validation of potential targets for the diagnosis, prevention, and treatment of tuberculosis. This review describes the various methods that are available for the generation of mutants in Mycobacterium species, focusing specifically on tools for altering slow-growing mycobacteria from the M. tb complex. Among others, it incorporates the recent new molecular biological technologies (e.g. ORBIT) to rapidly and/or genome-wide comprehensively obtain targeted mutants in mycobacteria. As such, this review can be used as a guide to select the appropriate genetic tools to generate mycobacterial mutants of interest, which can be used as tools to aid understanding of M. tb infection or to help developing TB intervention strategies.
Keywords
bacterial mutagenesis, gene editing, Mycobacterium, targeted mutagenesis, transposon mutagenesis, EFFICIENT ALLELIC EXCHANGE, GENE REPLACEMENT, BOVIS BCG, HOMOLOGOUS RECOMBINATION, TRANSPOSON MUTAGENESIS, BXB1 INTEGRATION, VIRULENCE GENES, IN-VIVO, TUBERCULOSIS, GENOME

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Please use this url to cite or link to this publication:

MLA
Borgers, Katlyn, et al. “A Guide to Mycobacterium Mutagenesis.” FEBS JOURNAL, vol. 286, no. 19, 2019, pp. 3757–74.
APA
Borgers, K., Vandewalle, K., Festjens, N., & Callewaert, N. (2019). A guide to Mycobacterium mutagenesis. FEBS JOURNAL, 286(19), 3757–3774.
Chicago author-date
Borgers, Katlyn, Kristof Vandewalle, Nele Festjens, and Nico Callewaert. 2019. “A Guide to Mycobacterium Mutagenesis.” FEBS JOURNAL 286 (19): 3757–74.
Chicago author-date (all authors)
Borgers, Katlyn, Kristof Vandewalle, Nele Festjens, and Nico Callewaert. 2019. “A Guide to Mycobacterium Mutagenesis.” FEBS JOURNAL 286 (19): 3757–3774.
Vancouver
1.
Borgers K, Vandewalle K, Festjens N, Callewaert N. A guide to Mycobacterium mutagenesis. FEBS JOURNAL. 2019;286(19):3757–74.
IEEE
[1]
K. Borgers, K. Vandewalle, N. Festjens, and N. Callewaert, “A guide to Mycobacterium mutagenesis,” FEBS JOURNAL, vol. 286, no. 19, pp. 3757–3774, 2019.
@article{8631422,
  abstract     = {The genus Mycobacterium includes several pathogens that cause severe disease in humans, like Mycobacterium tuberculosis (M. tb), the infectious agent causing tuberculosis. Genetic tools to engineer mycobacterial genomes, in a targeted or random fashion, have provided opportunities to investigate M. tb infection and pathogenesis. Furthermore, they have allowed the identification and validation of potential targets for the diagnosis, prevention, and treatment of tuberculosis. This review describes the various methods that are available for the generation of mutants in Mycobacterium species, focusing specifically on tools for altering slow-growing mycobacteria from the M. tb complex. Among others, it incorporates the recent new molecular biological technologies (e.g. ORBIT) to rapidly and/or genome-wide comprehensively obtain targeted mutants in mycobacteria. As such, this review can be used as a guide to select the appropriate genetic tools to generate mycobacterial mutants of interest, which can be used as tools to aid understanding of M. tb infection or to help developing TB intervention strategies.},
  author       = {Borgers, Katlyn and Vandewalle, Kristof and Festjens, Nele and Callewaert, Nico},
  issn         = {1742-464X},
  journal      = {FEBS JOURNAL},
  keywords     = {bacterial mutagenesis,gene editing,Mycobacterium,targeted mutagenesis,transposon mutagenesis,EFFICIENT ALLELIC EXCHANGE,GENE REPLACEMENT,BOVIS BCG,HOMOLOGOUS RECOMBINATION,TRANSPOSON MUTAGENESIS,BXB1 INTEGRATION,VIRULENCE GENES,IN-VIVO,TUBERCULOSIS,GENOME},
  language     = {eng},
  number       = {19},
  pages        = {3757--3774},
  title        = {A guide to Mycobacterium mutagenesis},
  url          = {http://dx.doi.org/10.1111/febs.15041},
  volume       = {286},
  year         = {2019},
}

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