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Enriched hydrogen-oxidizing microbiomes show a high diversity of co-existing hydrogen-oxidizing bacteria

Elham Ehsani, Charles Dumolin (UGent) , Jan Arends (UGent) , Frederiek-Maarten Kerckhof (UGent) , Xiaona Hu (UGent) , Peter Vandamme (UGent) and Nico Boon (UGent)
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Abstract
While numerous reports exist on the axenic culturing of different hydrogen-oxidizing bacteria (HOB), knowledge about the enrichment of microbial communities growing on hydrogen, oxygen, and carbon dioxide as sole carbon and energy sources remains negligible. We want to elucidate if in such enrichments, most enriched populations are HOBs or heterotrophic organisms. In the present study, bacteria enriched from a soil sample and grown over 5 transfers using a continuous supply of hydrogen, oxygen, and carbon dioxide to obtain an enriched autotrophic hydrogen-oxidizing microbiome. The success of the enrichment was evaluated by monitoring ammonium consumption and biomass concentration for 120 days. The shift in the microbial composition of the original soil inoculum and all transfers was observed based on 16S rRNA amplicon sequencing. The hydrogen-oxidizing facultative chemolithoautotroph Hydrogenophaga electricum was isolated and found to be one of the abundant species in most transfers. Moreover, Achromobacter was isolated both under heterotrophic and autotrophic conditions, which was characterized as a hydrogen-oxidizing bacterium. The HOB enrichment condition constructed in this study provided an environment for HOB to develop and conquer in all transfers. In conclusion, we showed that enrichments on hydrogen, oxygen, and carbon dioxide as sole carbon and energy sources contain a diverse mixture of HOB and heterotrophs that resulted in a collection of culturable isolates. These isolates can be useful for further investigation for industrial applications.
Keywords
Hydrogen-oxidizing bacteria, HOB enrichment, HOB isolation, Microbial composition, Heterotrophs, CARBON-DIOXIDE FIXATION, CRYSTAL-STRUCTURE, SP NOV., ESCHERICHIA-COLI, WATER, GROWTH, SOIL, DEGRADATION, CULTIVATION, PERFORMANCE

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MLA
Ehsani, Elham, et al. “Enriched Hydrogen-Oxidizing Microbiomes Show a High Diversity of Co-Existing Hydrogen-Oxidizing Bacteria.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 103, no. 19, 2019, pp. 8241–53, doi:10.1007/s00253-019-10082-z.
APA
Ehsani, E., Dumolin, C., Arends, J., Kerckhof, F.-M., Hu, X., Vandamme, P., & Boon, N. (2019). Enriched hydrogen-oxidizing microbiomes show a high diversity of co-existing hydrogen-oxidizing bacteria. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 103(19), 8241–8253. https://doi.org/10.1007/s00253-019-10082-z
Chicago author-date
Ehsani, Elham, Charles Dumolin, Jan Arends, Frederiek-Maarten Kerckhof, Xiaona Hu, Peter Vandamme, and Nico Boon. 2019. “Enriched Hydrogen-Oxidizing Microbiomes Show a High Diversity of Co-Existing Hydrogen-Oxidizing Bacteria.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103 (19): 8241–53. https://doi.org/10.1007/s00253-019-10082-z.
Chicago author-date (all authors)
Ehsani, Elham, Charles Dumolin, Jan Arends, Frederiek-Maarten Kerckhof, Xiaona Hu, Peter Vandamme, and Nico Boon. 2019. “Enriched Hydrogen-Oxidizing Microbiomes Show a High Diversity of Co-Existing Hydrogen-Oxidizing Bacteria.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103 (19): 8241–8253. doi:10.1007/s00253-019-10082-z.
Vancouver
1.
Ehsani E, Dumolin C, Arends J, Kerckhof F-M, Hu X, Vandamme P, et al. Enriched hydrogen-oxidizing microbiomes show a high diversity of co-existing hydrogen-oxidizing bacteria. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2019;103(19):8241–53.
IEEE
[1]
E. Ehsani et al., “Enriched hydrogen-oxidizing microbiomes show a high diversity of co-existing hydrogen-oxidizing bacteria,” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 103, no. 19, pp. 8241–8253, 2019.
@article{8630873,
  abstract     = {{While numerous reports exist on the axenic culturing of different hydrogen-oxidizing bacteria (HOB), knowledge about the enrichment of microbial communities growing on hydrogen, oxygen, and carbon dioxide as sole carbon and energy sources remains negligible. We want to elucidate if in such enrichments, most enriched populations are HOBs or heterotrophic organisms. In the present study, bacteria enriched from a soil sample and grown over 5 transfers using a continuous supply of hydrogen, oxygen, and carbon dioxide to obtain an enriched autotrophic hydrogen-oxidizing microbiome. The success of the enrichment was evaluated by monitoring ammonium consumption and biomass concentration for 120 days. The shift in the microbial composition of the original soil inoculum and all transfers was observed based on 16S rRNA amplicon sequencing. The hydrogen-oxidizing facultative chemolithoautotroph Hydrogenophaga electricum was isolated and found to be one of the abundant species in most transfers. Moreover, Achromobacter was isolated both under heterotrophic and autotrophic conditions, which was characterized as a hydrogen-oxidizing bacterium. The HOB enrichment condition constructed in this study provided an environment for HOB to develop and conquer in all transfers. In conclusion, we showed that enrichments on hydrogen, oxygen, and carbon dioxide as sole carbon and energy sources contain a diverse mixture of HOB and heterotrophs that resulted in a collection of culturable isolates. These isolates can be useful for further investigation for industrial applications.}},
  author       = {{Ehsani, Elham and Dumolin, Charles and Arends, Jan and Kerckhof, Frederiek-Maarten and Hu, Xiaona and Vandamme, Peter and Boon, Nico}},
  issn         = {{0175-7598}},
  journal      = {{APPLIED MICROBIOLOGY AND BIOTECHNOLOGY}},
  keywords     = {{Hydrogen-oxidizing bacteria,HOB enrichment,HOB isolation,Microbial composition,Heterotrophs,CARBON-DIOXIDE FIXATION,CRYSTAL-STRUCTURE,SP NOV.,ESCHERICHIA-COLI,WATER,GROWTH,SOIL,DEGRADATION,CULTIVATION,PERFORMANCE}},
  language     = {{eng}},
  number       = {{19}},
  pages        = {{8241--8253}},
  title        = {{Enriched hydrogen-oxidizing microbiomes show a high diversity of co-existing hydrogen-oxidizing bacteria}},
  url          = {{http://dx.doi.org/10.1007/s00253-019-10082-z}},
  volume       = {{103}},
  year         = {{2019}},
}

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