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Assessment of dried blood spots for multi-mycotoxin biomarker analysis in pigs and broiler chickens

(2019) TOXINS. 11(9).
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Abstract
Dried blood spots (DBSs), a micro-sampling technique whereby a drop of blood is collected on filter paper has multiple advantages over conventional blood sampling regarding the sampling itself, as well as transportation and storage. This is the first paper describing the development and validation of a method for the determination of 23 mycotoxins and phase I metabolites in DBSs from pigs and broiler chickens using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The targeted mycotoxins belong to groups for which the occurrence in feed is regulated by the European Union, namely, aflatoxins, ochratoxin A and several Fusarium mycotoxins, and to two groups of unregulated mycotoxins, namely Alternaria mycotoxins and Fusarium mycotoxins (enniatins and beauvericin). The impact of blood haematocrit, DBS sampling volume and size of the analysed DBS disk on the validation results was assessed. No effects of variation in size of the analysed disk, haematocrit and spotted blood volume were observed for most mycotoxins, except for the aflatoxins and beta-zearalanol (BZAL) at the lowest haematocrit (26%) level and for the enniatins (ENNs) at the lowest volume (40 mu L). The developed method was transferred to an LC-high resolution mass spectrometry instrument to determine phase II metabolites. Then, the DBS technique was applied in a proof-of-concept toxicokinetic study including a comparison with LC-MS/MS data from plasma obtained with conventional venous blood sampling. A strong correlation (r > 0.947) was observed between plasma and DBS concentrations. Finally, DBSs were also applied in a pilot exposure assessment study to test their applicability under field conditions.
Keywords
dried blood spots, pigs, broiler chickens, multi-mycotoxin analysis, toxicokinetic study, exposure assessment, biomarkers for exposure, QUANTITATIVE-DETERMINATION, COMPARATIVE TOXICOKINETICS, OCHRATOXIN, HEMATOCRIT, STRATEGIES, COFFEE, FEED

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Citation

Please use this url to cite or link to this publication:

MLA
Lauwers, Marianne, et al. “Assessment of Dried Blood Spots for Multi-Mycotoxin Biomarker Analysis in Pigs and Broiler Chickens.” TOXINS, vol. 11, no. 9, 2019.
APA
Lauwers, M., Croubels, S., De Baere, S., Sevastiyanova, M., Romera Sierra, E. M., Letor, B., … Devreese, M. (2019). Assessment of dried blood spots for multi-mycotoxin biomarker analysis in pigs and broiler chickens. TOXINS, 11(9).
Chicago author-date
Lauwers, Marianne, Siska Croubels, Siegrid De Baere, Milena Sevastiyanova, Eva Maria Romera Sierra, Ben Letor, Christos Gougoulias, and Mathias Devreese. 2019. “Assessment of Dried Blood Spots for Multi-Mycotoxin Biomarker Analysis in Pigs and Broiler Chickens.” TOXINS 11 (9).
Chicago author-date (all authors)
Lauwers, Marianne, Siska Croubels, Siegrid De Baere, Milena Sevastiyanova, Eva Maria Romera Sierra, Ben Letor, Christos Gougoulias, and Mathias Devreese. 2019. “Assessment of Dried Blood Spots for Multi-Mycotoxin Biomarker Analysis in Pigs and Broiler Chickens.” TOXINS 11 (9).
Vancouver
1.
Lauwers M, Croubels S, De Baere S, Sevastiyanova M, Romera Sierra EM, Letor B, et al. Assessment of dried blood spots for multi-mycotoxin biomarker analysis in pigs and broiler chickens. TOXINS. 2019;11(9).
IEEE
[1]
M. Lauwers et al., “Assessment of dried blood spots for multi-mycotoxin biomarker analysis in pigs and broiler chickens,” TOXINS, vol. 11, no. 9, 2019.
@article{8629764,
  abstract     = {Dried blood spots (DBSs), a micro-sampling technique whereby a drop of blood is collected on filter paper has multiple advantages over conventional blood sampling regarding the sampling itself, as well as transportation and storage. This is the first paper describing the development and validation of a method for the determination of 23 mycotoxins and phase I metabolites in DBSs from pigs and broiler chickens using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The targeted mycotoxins belong to groups for which the occurrence in feed is regulated by the European Union, namely, aflatoxins, ochratoxin A and several Fusarium mycotoxins, and to two groups of unregulated mycotoxins, namely Alternaria mycotoxins and Fusarium mycotoxins (enniatins and beauvericin). The impact of blood haematocrit, DBS sampling volume and size of the analysed DBS disk on the validation results was assessed. No effects of variation in size of the analysed disk, haematocrit and spotted blood volume were observed for most mycotoxins, except for the aflatoxins and beta-zearalanol (BZAL) at the lowest haematocrit (26%) level and for the enniatins (ENNs) at the lowest volume (40 mu L). The developed method was transferred to an LC-high resolution mass spectrometry instrument to determine phase II metabolites. Then, the DBS technique was applied in a proof-of-concept toxicokinetic study including a comparison with LC-MS/MS data from plasma obtained with conventional venous blood sampling. A strong correlation (r > 0.947) was observed between plasma and DBS concentrations. Finally, DBSs were also applied in a pilot exposure assessment study to test their applicability under field conditions.},
  articleno    = {541},
  author       = {Lauwers, Marianne and Croubels, Siska and De Baere, Siegrid and Sevastiyanova, Milena and Romera Sierra, Eva Maria and Letor, Ben and Gougoulias, Christos and Devreese, Mathias},
  issn         = {2072-6651},
  journal      = {TOXINS},
  keywords     = {dried blood spots,pigs,broiler chickens,multi-mycotoxin analysis,toxicokinetic study,exposure assessment,biomarkers for exposure,QUANTITATIVE-DETERMINATION,COMPARATIVE TOXICOKINETICS,OCHRATOXIN,HEMATOCRIT,STRATEGIES,COFFEE,FEED},
  language     = {eng},
  number       = {9},
  pages        = {22},
  title        = {Assessment of dried blood spots for multi-mycotoxin biomarker analysis in pigs and broiler chickens},
  url          = {http://dx.doi.org/10.3390/toxins11090541},
  volume       = {11},
  year         = {2019},
}

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