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A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes

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Abstract
Lasiodiplodia theobromae (Botryosphaeriaceae, Ascomycota) is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. The molecular effects of temperature on L. theobromae are mostly unknown, so we used a multi-omics approach to understand how temperature affects the molecular mechanisms of pathogenicity. The genome of L. theobromae LA-SOL3 was sequenced (Illumina MiSeq) and annotated. Furthermore, the transcriptome (Illumina TruSeq) and proteome (Orbitrap LC-MS/MS) of LA-SOL3 grown at 25 degrees C and 37 degrees C were analysed. Proteins related to pathogenicity (plant cell wall degradation, toxin synthesis, mitogen-activated kinases pathway and proteins involved in the velvet complex) were more abundant when the fungus grew at 25 degrees C. At 37 degrees C, proteins related to pathogenicity were less abundant than at 25 degrees C, while proteins related to cell wall organisation were more abundant. On the other hand, virulence factors involved in human pathogenesis, such as the SSD1 virulence protein, were expressed only at 37 degrees C. Taken together, our results showed that this species presents a typical phytopathogenic molecular profile that is compatible with a hemibiotrophic lifestyle. We showed that L. theobromae is equipped with the pathogenesis toolbox that enables it to infect not only plants but also animals.
Keywords
CLIMATE-CHANGE, PLANT, KINASE, FUNGUS, CLASSIFICATION, IDENTIFICATION, BIOSYNTHESIS, SECRETOME, PATHWAYS, DISEASES

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MLA
Felix, Carina et al. “A Multi-omics Analysis of the Grapevine Pathogen Lasiodiplodia Theobromae Reveals That Temperature Affects the Expression of Virulence- and Pathogenicity-related Genes.” SCIENTIFIC REPORTS 9 (2019): n. pag. Print.
APA
Felix, Carina, Silva Meneses, R., Goncalves, M. F. M., Tilleman, L., Duarte, A. S., Jorrin-Novo, J. V., Van de Peer, Y., et al. (2019). A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes. SCIENTIFIC REPORTS, 9.
Chicago author-date
Felix, Carina, Rodrigo Silva Meneses, Micael F. M. Goncalves, Laurentijn Tilleman, Ana S. Duarte, Jesus V. Jorrin-Novo, Yves Van de Peer, et al. 2019. “A Multi-omics Analysis of the Grapevine Pathogen Lasiodiplodia Theobromae Reveals That Temperature Affects the Expression of Virulence- and Pathogenicity-related Genes.” Scientific Reports 9.
Chicago author-date (all authors)
Felix, Carina, Rodrigo Silva Meneses, Micael F. M. Goncalves, Laurentijn Tilleman, Ana S. Duarte, Jesus V. Jorrin-Novo, Yves Van de Peer, Dieter Deforce, Filip Van Nieuwerburgh, Ana C. Esteves, and Artur Alves. 2019. “A Multi-omics Analysis of the Grapevine Pathogen Lasiodiplodia Theobromae Reveals That Temperature Affects the Expression of Virulence- and Pathogenicity-related Genes.” Scientific Reports 9.
Vancouver
1.
Felix C, Silva Meneses R, Goncalves MFM, Tilleman L, Duarte AS, Jorrin-Novo JV, et al. A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes. SCIENTIFIC REPORTS. 2019;9.
IEEE
[1]
C. Felix et al., “A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes,” SCIENTIFIC REPORTS, vol. 9, 2019.
@article{8629672,
  abstract     = {Lasiodiplodia theobromae (Botryosphaeriaceae, Ascomycota) is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. The molecular effects of temperature on L. theobromae are mostly unknown, so we used a multi-omics approach to understand how temperature affects the molecular mechanisms of pathogenicity. The genome of L. theobromae LA-SOL3 was sequenced (Illumina MiSeq) and annotated. Furthermore, the transcriptome (Illumina TruSeq) and proteome (Orbitrap LC-MS/MS) of LA-SOL3 grown at 25 degrees C and 37 degrees C were analysed. Proteins related to pathogenicity (plant cell wall degradation, toxin synthesis, mitogen-activated kinases pathway and proteins involved in the velvet complex) were more abundant when the fungus grew at 25 degrees C. At 37 degrees C, proteins related to pathogenicity were less abundant than at 25 degrees C, while proteins related to cell wall organisation were more abundant. On the other hand, virulence factors involved in human pathogenesis, such as the SSD1 virulence protein, were expressed only at 37 degrees C. Taken together, our results showed that this species presents a typical phytopathogenic molecular profile that is compatible with a hemibiotrophic lifestyle. We showed that L. theobromae is equipped with the pathogenesis toolbox that enables it to infect not only plants but also animals.},
  articleno    = {13144},
  author       = {Felix, Carina and Silva Meneses, Rodrigo and Goncalves, Micael F. M. and Tilleman, Laurentijn and Duarte, Ana S. and Jorrin-Novo, Jesus V. and Van de Peer, Yves and Deforce, Dieter and Van Nieuwerburgh, Filip and Esteves, Ana C. and Alves, Artur},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {CLIMATE-CHANGE,PLANT,KINASE,FUNGUS,CLASSIFICATION,IDENTIFICATION,BIOSYNTHESIS,SECRETOME,PATHWAYS,DISEASES},
  language     = {eng},
  pages        = {12},
  title        = {A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes},
  url          = {http://dx.doi.org/10.1038/s41598-019-49551-w},
  volume       = {9},
  year         = {2019},
}

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