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Differential efficiency of wild type rhizogenic strains for rol gene transformation of plants

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Abstract
Rhizogenic agrobacteria induce extensive root proliferation, in several economically valuable, dicotyledonous plant species, a phenomenon referred to as hairy roots. Besides their pathogenic nature, agrobacteria have proven to be a valuable asset in biotechnology and molecular plant breeding. To assess the potential of frequently used rhizogenic strains, growth in yeast extract glucose broth and antibiotic resistance was analyzed. Growth curves were established for Arqua1, NCPPB2659, LMG150, LMG152, and ATCC15834; and regression analysis of the exponential growth phase resulted in a reliable and standardized method for preparation of a bacterial suspension for inoculation. Cell density did not correlate with the timing of hairy root emergence. The highest number of hairy roots was obtained with an inoculum of 1x10(8) CFUml(-1) for Arqua1, NCPPB2659, and LMG152. Cell density of ATCC15834 did not affect the number of hairy roots formed. The identity of the rhizogenic strains for plant transformation was verified in phylogenetic analysis using average nucleotide identity (ANI), which also provided insight in their genetic diversity within the Rhizobium taxon.
Keywords
Agrobacterium, Average nucleotide identity, Cell density, Growth curve, Hairy root, Ri plasmid, AGROBACTERIUM-MEDIATED TRANSFORMATION, TRANSGENIC HAIRY ROOTS, T-DNA, VIRULENCE GENES, RI-PLASMID, CROWN-GALL, BIOVAR 1, INDUCTION, OPINE, REGENERATION

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MLA
Desmet, Siel, et al. “Differential Efficiency of Wild Type Rhizogenic Strains for Rol Gene Transformation of Plants.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 103, no. 16, 2019, pp. 6657–72.
APA
Desmet, S., De Keyser, E., Van Vaerenbergh, J., Baeyen, S., Van Huylenbroeck, J., Geelen, D., & Dhooghe, E. (2019). Differential efficiency of wild type rhizogenic strains for rol gene transformation of plants. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 103(16), 6657–6672.
Chicago author-date
Desmet, Siel, Ellen De Keyser, Johan Van Vaerenbergh, Steve Baeyen, Johan Van Huylenbroeck, Danny Geelen, and Emmy Dhooghe. 2019. “Differential Efficiency of Wild Type Rhizogenic Strains for Rol Gene Transformation of Plants.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103 (16): 6657–72.
Chicago author-date (all authors)
Desmet, Siel, Ellen De Keyser, Johan Van Vaerenbergh, Steve Baeyen, Johan Van Huylenbroeck, Danny Geelen, and Emmy Dhooghe. 2019. “Differential Efficiency of Wild Type Rhizogenic Strains for Rol Gene Transformation of Plants.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103 (16): 6657–6672.
Vancouver
1.
Desmet S, De Keyser E, Van Vaerenbergh J, Baeyen S, Van Huylenbroeck J, Geelen D, et al. Differential efficiency of wild type rhizogenic strains for rol gene transformation of plants. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2019;103(16):6657–72.
IEEE
[1]
S. Desmet et al., “Differential efficiency of wild type rhizogenic strains for rol gene transformation of plants,” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 103, no. 16, pp. 6657–6672, 2019.
@article{8629352,
  abstract     = {Rhizogenic agrobacteria induce extensive root proliferation, in several economically valuable, dicotyledonous plant species, a phenomenon referred to as hairy roots. Besides their pathogenic nature, agrobacteria have proven to be a valuable asset in biotechnology and molecular plant breeding. To assess the potential of frequently used rhizogenic strains, growth in yeast extract glucose broth and antibiotic resistance was analyzed. Growth curves were established for Arqua1, NCPPB2659, LMG150, LMG152, and ATCC15834; and regression analysis of the exponential growth phase resulted in a reliable and standardized method for preparation of a bacterial suspension for inoculation. Cell density did not correlate with the timing of hairy root emergence. The highest number of hairy roots was obtained with an inoculum of 1x10(8) CFUml(-1) for Arqua1, NCPPB2659, and LMG152. Cell density of ATCC15834 did not affect the number of hairy roots formed. The identity of the rhizogenic strains for plant transformation was verified in phylogenetic analysis using average nucleotide identity (ANI), which also provided insight in their genetic diversity within the Rhizobium taxon.},
  author       = {Desmet, Siel and De Keyser, Ellen and Van Vaerenbergh, Johan and Baeyen, Steve and Van Huylenbroeck, Johan and Geelen, Danny and Dhooghe, Emmy},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keywords     = {Agrobacterium,Average nucleotide identity,Cell density,Growth curve,Hairy root,Ri plasmid,AGROBACTERIUM-MEDIATED TRANSFORMATION,TRANSGENIC HAIRY ROOTS,T-DNA,VIRULENCE GENES,RI-PLASMID,CROWN-GALL,BIOVAR 1,INDUCTION,OPINE,REGENERATION},
  language     = {eng},
  number       = {16},
  pages        = {6657--6672},
  title        = {Differential efficiency of wild type rhizogenic strains for rol gene transformation of plants},
  url          = {http://dx.doi.org/10.1007/s00253-019-10003-0},
  volume       = {103},
  year         = {2019},
}

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