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Illegitimate integration of non-replicative vectors in the genome of Rhodoccus fascians upon electro-transformation as an insertional mutagenesis system

(1991) MOLECULAR MICROBIOLOGY. 5(9). p.2115-2124
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Abstract
Electrotransformation of Rhodococcus fascians by non-replicating plasmids containing a suitable resistance marker resulted in stable transformants by integration of these constructs at various sites in the genome, thereby generating different mutations. Tagged genes could be isolated in Escherichia coli owing to the presence of a ColE1 replicon and an ampicillin resistance gene in the inserted sequences. Southern analysis and nucleotide sequencing revealed that recombination can occur at defined locations in the plasmid, while no site preference for target sequences could be detected. Low homology between the recombining sequences indicates illegitimate recombination. The specificity of the plasmid sites could be explained by assuming a linear recombination intermediate, generated by cleavage of the transformed plasmid.
Keywords
Bacillus subtilis, Corynebacterium fascians, Cloning vehicles, Escherichia coli, DNA, Plasmid, Streptomyces, Electroporesis

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Citation

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MLA
Desomer, Jan, et al. “Illegitimate Integration of Non-Replicative Vectors in the Genome of Rhodoccus Fascians upon Electro-Transformation as an Insertional Mutagenesis System.” MOLECULAR MICROBIOLOGY, vol. 5, no. 9, 1991, pp. 2115–24.
APA
Desomer, J., Crespi, M., & Van Montagu, M. (1991). Illegitimate integration of non-replicative vectors in the genome of Rhodoccus fascians upon electro-transformation as an insertional mutagenesis system. MOLECULAR MICROBIOLOGY, 5(9), 2115–2124.
Chicago author-date
Desomer, Jan, Martin Crespi, and Marc Van Montagu. 1991. “Illegitimate Integration of Non-Replicative Vectors in the Genome of Rhodoccus Fascians upon Electro-Transformation as an Insertional Mutagenesis System.” MOLECULAR MICROBIOLOGY 5 (9): 2115–24.
Chicago author-date (all authors)
Desomer, Jan, Martin Crespi, and Marc Van Montagu. 1991. “Illegitimate Integration of Non-Replicative Vectors in the Genome of Rhodoccus Fascians upon Electro-Transformation as an Insertional Mutagenesis System.” MOLECULAR MICROBIOLOGY 5 (9): 2115–2124.
Vancouver
1.
Desomer J, Crespi M, Van Montagu M. Illegitimate integration of non-replicative vectors in the genome of Rhodoccus fascians upon electro-transformation as an insertional mutagenesis system. MOLECULAR MICROBIOLOGY. 1991;5(9):2115–24.
IEEE
[1]
J. Desomer, M. Crespi, and M. Van Montagu, “Illegitimate integration of non-replicative vectors in the genome of Rhodoccus fascians upon electro-transformation as an insertional mutagenesis system,” MOLECULAR MICROBIOLOGY, vol. 5, no. 9, pp. 2115–2124, 1991.
@article{8628117,
  abstract     = {{Electrotransformation of Rhodococcus fascians by non-replicating plasmids containing a suitable resistance marker resulted in stable transformants by integration of these constructs at various sites in the genome, thereby generating different mutations. Tagged genes could be isolated in Escherichia coli owing to the presence of a ColE1 replicon and an ampicillin resistance gene in the inserted sequences. 
Southern analysis and nucleotide sequencing revealed that recombination can occur at defined locations in the plasmid, while no site preference for target sequences could be detected. Low homology between the recombining sequences indicates illegitimate recombination. The specificity of the plasmid sites could be explained by assuming a linear recombination intermediate, generated by cleavage of the transformed plasmid.}},
  author       = {{Desomer, Jan and Crespi, Martin and Van Montagu, Marc}},
  issn         = {{0950-382X}},
  journal      = {{MOLECULAR MICROBIOLOGY}},
  keywords     = {{Bacillus subtilis,Corynebacterium fascians,Cloning vehicles,Escherichia coli,DNA,Plasmid,Streptomyces,Electroporesis}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{2115--2124}},
  title        = {{Illegitimate integration of non-replicative vectors in the genome of Rhodoccus fascians upon electro-transformation as an insertional mutagenesis system}},
  url          = {{http://dx.doi.org/10.1111/j.1365-2958.1991.tb02141.x}},
  volume       = {{5}},
  year         = {{1991}},
}

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